Exploiting fluorescent polymers to probe the self-assembly of virus-like particles

The inside surfaces of the protein shells of many viruses are positively charged, thereby enhancing the self-assembly of capsid proteins around their (oppositely charged) RNA genome. These proteins have been shown to organize similarly around a variety of nonbiological, negatively charged, polymers, for example, poly(styrene sulfonate) (PSS), forming virus-like particles (VLPs). We have demonstrated recently that the VLPs formed from cowpea chlorotic mottle virus (CCMV) capsid protein increase in size (from T=2 to T=3 structures) upon increase in PSS molecular weight (from 400 kDa to 3.4 MDa), and that the total charge on the PSS exceeds that of the capsid protein by as much as a factor of 9. Here, we extend studies of this kind to PSS molecules that are sufficiently small that two or more can be packaged into VLPs. The use of 38 kDa PSS polymers that have been fluorescently labeled with Rhodamine B allows us to determine the number of PSS molecules per capsid. Electron micrographs of the VLPs show a bimodal distribution of particle diameters, with one peak centered around 19 nm, typical of a T=1 triangulation number, and the other around 21 nm, consistent with a pseudo T=2 structure; increasing the molar ratio of protein to PSS in the reaction mix shifts the VLP distribution from T=1 to T=2 structures. By combining fluorescence and gel electrophoresis measurements, it is determined that, on average, there are two polymers in each T=1 capsid and three in each T=2, with the PSS charge less than that of the capsid protein by as much as a factor of 2. VLPs of this kind provide a versatile model system for determining the principles underlying self-assembly of controlled numbers of cargo molecules in nanocontainers of increasing size

Statistical modeling of RNA structure profiling experiments enables parsimonious reconstruction of structure landscapes

Different experimental and computational approaches can be used to study RNA structures. Here, the authors present a computational method for data-directed reconstruction of complex RNA structure landscapes, which predicts a parsimonious set of co-existing structures and estimates their abundances from structure profiling data

Electoral cycles in MPs’ salaries: evidence from the German states

Members of parliament (MPs) often decide on their own salaries. Voters dislike self-serving politicians, and politicians are keen to gratify their voters. In line with the political business cycle theories, politicians thus may well delay deciding on increases in salaries until after elections. We investigate electoral cycles in the salary increases of German state MPs. Using data for 15 states over the period 1980-2014, the results do not show that decisions on increases in MPs’ salaries were influenced by elections. In fact, MPs’ salaries increased by 0.30 to 0.37 percent when employees’ salaries increased by one percent. Politicians can increase their salaries at any point of time in the legislative period: understanding that all politicians benefit from an increase in salaries, voters may only be disenchanted with politics in general, without punishing individual incumbent parties