ENDOPARASITES OF GREATER SANDHILL CRANES IN SOUTH-CENTRAL WISCONSIN

Windingstad and Trainer (1977) used both fecal sampling and postmortem examinations to document the occurrence of parasites in greater sandhill cranes (Grus canadensis tabida) from Wisconsin in the fall. We conducted repeated fecal sampling of a well-known population to expand on results of their study. Our objective was to determine whether seasonal differences exist in the prevalence of endoparasites of Wisconsin sandhill cranes. We collected 7 to 10 fecal samples approximately every other week from a consistent roost site on the Wisconsin River (43°34\u2752.99\u27\u27N, 89°36\u2738.42\u27\u27W) near Briggsville, Wisconsin, from 29 May through 25 September 2008. The sample size was based on the assumption that endoparasite prevalence in this population was high: a single positive result would allow us to be 99% certain that the parasite was prevalent in 50% or greater of the crane population (Martin et al. 1987). Each anonymously collected fecal sample consisted of a single, fresh mass. Samples were collected into plastic bags and kept refrigerated until analysis (2-24 hours later). Three methods were used to detect parasites: a standard direct smear of feces in saline, fecal flotation in sodium nitrate solution (Ovatector, BGS Medical Products, Inc, Venice, FL.) (Greiner 1997), and examination of the uppermost layer of sediment 10 minutes following mixing of the sample with sodium nitrate

ENDOPARASITES OF GREATER SANDHILL CRANES IN SOUTH-CENTRAL WISCONSIN

Windingstad and Trainer (1977) used both fecal sampling and postmortem examinations to document the occurrence of parasites in greater sandhill cranes (Grus canadensis tabida) from Wisconsin in the fall. We conducted repeated fecal sampling of a well-known population to expand on results of their study. Our objective was to determine whether seasonal differences exist in the prevalence of endoparasites of Wisconsin sandhill cranes. We collected 7 to 10 fecal samples approximately every other week from a consistent roost site on the Wisconsin River (43°34\u2752.99\u27\u27N, 89°36\u2738.42\u27\u27W) near Briggsville, Wisconsin, from 29 May through 25 September 2008. The sample size was based on the assumption that endoparasite prevalence in this population was high: a single positive result would allow us to be 99% certain that the parasite was prevalent in 50% or greater of the crane population (Martin et al. 1987). Each anonymously collected fecal sample consisted of a single, fresh mass. Samples were collected into plastic bags and kept refrigerated until analysis (2-24 hours later). Three methods were used to detect parasites: a standard direct smear of feces in saline, fecal flotation in sodium nitrate solution (Ovatector, BGS Medical Products, Inc, Venice, FL.) (Greiner 1997), and examination of the uppermost layer of sediment 10 minutes following mixing of the sample with sodium nitrate

THE EFFECTS OF ANTICOAGULANT CHOICE AND SAMPLE PROCESSING TIME ON HEMATOLOGIC VALUES OF JUVENILE WHOOPING CRANES

Blood collected from juvenile whooping cranes (Grus americana) in 2007 and 2008 was divided and placed in either the anticoagulant lithium heparin (LiHep) or tri-potassium ethylenediaminetetraacetic acid (K3EDTA) for diagnostic hematology. Thin smears were prepared from the anticoagulated blood in the field with no delay and in the laboratory after a 4-6-hour delay, and then used to determine differential and total leukocyte counts. Manual heterophil and eosinophil counts were greater in LiHep-treated samples compared to K3EDTA samples (P \u3c 0.05), but there was no difference in the total leukocyte concentration or differential leukocyte counts between anticoagulants based on blood smears prepared with no delay (n = 15). Marked differences were noted in relative heterophil (P \u3c 0.05) and lymphocyte (P \u3c 0.05) counts and total leukocyte (P \u3c 0.05) concentrations of K3EDTA-treated samples processed after the delay (n = 7), suggesting a negative effect on lymphocyte integrity from the anticoagulant. Microscopically, lymphocytes were more intact and easily differentiated from thrombocytes in LiHep-treated samples than K3EDTA, but modest thrombocyte clumping in the LiHep samples was a concern. Either anticoagulant appears adequate for diagnostic hematology in juvenile whooping cranes based on this limited analysis, but blood smears should be prepared immediately under controlled conditions for best results