298 research outputs found
Small oligonucleotides detection in three-dimensional polymer network of dna-peg hydrogels
The control of the three-dimensional (3D) polymer network structure is important for permselective materials when specific biomolecule detection is needed. Here we investigate conditions to obtain a tailored hydrogel network that combines both molecular filtering and molecular capture capabilities for biosensing applications. Along this line, short oligonucleotide detection in a displacement assay is set within PEGDA hydrogels synthetized by UV radical photopolymerization. To provide insights on the molecular filter capability, diffusion studies of several probes (sulforho-damine G and dextrans) with different hydrodynamic radii were carried out using NMR technique. Moreover, fluorometric analyses of hybridization of DNA oligonucleotides inside PEGDA hydrogels shed light on the mechanisms of recognition in 3D, highlighting that mesh size and crowding effect greatly impact the hybridization mechanism on a polymer network. Finally, we found the best probe density and diffusion transport conditions to allow the specific oligonucleotide capture and detection inside PEGDA hydrogels for oligonucleotide detection and the filtering out of higher molecular weight molecules
Anomalous enhancements of low-energy fusion rates in plasmas: the role of ion momentum distributions and inhomogeneous screening
Non-resonant fusion cross-sections significantly higher than corresponding
theoretical predictions are observed in low-energy experiments with deuterated
matrix target. Models based on thermal effects, electron screening, or
quantum-effect dispersion relations have been proposed to explain these
anomalous results: none of them appears to satisfactory reproduce the
experiments. Velocity distributions are fundamental for the reaction rates and
deviations from the Maxwellian limit could play a central role in explaining
the enhancement. We examine two effects: an increase of the tail of the target
Deuteron momentum distribution due to the Galitskii-Yakimets quantum
uncertainty effect, which broadens the energy-momentum relation; and spatial
fluctuations of the Debye-H\"{u}ckel radius leading to an effective increase of
electron screening. Either effect leads to larger reaction rates especially
large at energies below a few keV, reducing the discrepancy between
observations and theoretical expectations.Comment: 6 pages, 3 figure
Screening enhancement factors for laboratory CNO and rp astrophysical reactions
Cross sections of laboratory CNO and rp astrophysical reactions are enhanced
due to the presence of the multi-electron cloud that surrounds the target
nuclei. As a result the relevant astrophysical factors are overestimated unless
corrected appropriately. This study gives both an estimate of the error
committed if screening effects are not taken into account and a rough profile
of the laboratory energy thresholds at which the screening effect appears. The
results indicate that, for most practical purposes, screening corrections to
past relevant experiments can be disregarded. Regarding future experiments,
however, screening corrections to the CNO reactions will certainly be of
importance as they are closely related to the solar neutrino fluxes and the rp
process. Moreover, according to the present results, screening effects will
have to be taken into account particularly by the current and future LUNA
experiments, where screened astrophysical factors will be enhanced to a
significant degree.Comment: 6 RevTex pages + 2 ps figures. (Revised version). Accepted for
publication in Journal of Physics
Enhancement of fusion rates due to quantum effects in the particles momentum distribution in nonideal media
This study concerns a situation when measurements of the nonresonant
cross-section of nuclear reactions appear highly dependent on the environment
in which the particles interact. An appealing example discussed in the paper is
the interaction of a deuteron beam with a target of deuterated metal Ta. In
these experiments, the reaction cross section for d(d,p)t was shown to be
orders of magnitude greater than what the conventional model predicts for the
low-energy particles. In this paper we take into account the influence of
quantum effects due to the Heisenberg uncertainty principle for particles in a
non-ideal medium elastically interacting with the medium particles. In order to
calculate the nuclear reaction rate in the non-ideal environment we apply both
the Monte Carlo technique and approximate analytical calculation of the Feynman
diagram using nonrelativistic kinetic Green's functions in the medium which
correspond to the generalized energy and momentum distribution functions of
interacting particles. We show a possibility to reduce the 12-fold integral
corresponding to this diagram to a fivefold integral. This can significantly
speed up the computation and control accuracy. Our calculations show that
quantum effects significantly influence reaction rates such as p +7Be, 3He
+4He, p +7Li, and 12C +12C. The new reaction rates may be much higher than the
classical ones for the interior of the Sun and supernova stars. The possibility
to observe the theoretical predictions under laboratory conditions is
discussed
CD19-Targeted Immunotherapies for Diffuse Large B-Cell Lymphoma
Surgical resection, chemotherapy and radiotherapy were, for many years, the only available cancer treatments. Recently, the use of immune checkpoint inhibitors and adoptive cell therapies has emerged as promising alternative. These cancer immunotherapies are aimed to support or harness the patient\u2019s immune system to recognize and destroy cancer cells. Preclinical and clinical studies, based on the use of T cells and more recently NK cells genetically modified with chimeric antigen receptors retargeting the adoptive cell therapy towards tumor cells, have already shown remarkable results. In this review, we outline the latest highlights and progress in immunotherapies for the treatment of Diffuse Large B-cell Lymphoma (DLBCL) patients, focusing on CD19-targeted immunotherapies. We also discuss current clinical trials and opportunities of using immunotherapies to treat DLBCL patients
Constraints on energetic particles in the Fleischmann-Pons experiment
In recent Fleischmann–Pons experiments carried out by different groups, a thermal signal is seen indicative of excess energy production of a magnitude much greater than can be accounted for by chemistry. Correlated with the excess heat appears to be 4He, with the associated energy near 24 MeV per helium atom. In nuclear reactions, the energy produced is expressed through the kinetic energy of the products; hence, it would be natural to assume that some of the reaction energy ends up as kinetic energy of the 4He nucleus. Depending on the energy that the helium nucleus is born with, it will result in radiation (such as neutrons or x-rays) that can be seen outside of the cell. We have computed estimates of the expected neutron and x-ray emission as a function of helium energy and compared the results with upper limits taken from experiments. Experimental results with upper limits of neutron emission between 0.008 and 0.8 n/J are found to correspond to upper limits in alpha energy between 6.2 and 20.2 keV
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