Esophageal acid stimulation alters insular cortex functional connectivity in gastroesophageal reflux disease

BACKGROUND: The insula plays a significant role in the interoceptive processing of visceral stimuli. We have previously shown that gastroesophageal reflux disease (GERD) patients have increased insular cortex activity during esophageal stimulation, suggesting a sensitized esophago-cortical neuraxis. However, information regarding the functional connectivity (FC) of the insula during visceral stimulation is lacking. The primary aim of this study was to investigate the FC of insular subregions during esophageal acid stimulation. METHODS: Functional imaging data were obtained from 12 GERD patients and 14 healthy subjects during four steady state conditions: (i) presence of transnasal esophageal catheter (pre-infusion); (ii) neutral solution; (iii) acid infusion; (iv) presence of transnasal esophageal catheter following infusions (post-infusion). The insula was parcellated into six regions of interest. FC maps between each insular ROI and interoceptive regions were created. Differences in FC between GERD patients and healthy subjects were determined across the 4 study conditions. KEY RESULTS: All GERD patients experienced heartburn during and after esophageal acidification. Significant differences between GERD patients and healthy subjects were seen in: (i) insula-thalamic FC (neutral solution infusion, acid infusion, post-infusion); (ii) insula-amygdala FC (acid infusion, post-infusion); (iii) insula-hippocampus and insula-cingulate FC (post-infusion). CONCLUSIONS & INFERENCES: Esophageal stimulation in GERD patients revealed significant insular cortex FC differences with regions involved in viscerosensation and interoception. The results of our study provide further evidence that the insula, located at the transition of afferent physiologic information to human feelings, is essential for both visceral homeostasis and the experience of heartburn in GERD patients

Image Content Analysis Using Neural Networks and Genetic Algorithms

The analysis of digital images for content discovery is a process of identifying and classifying patterns and sub-images that can lead to recognizing contents of the processed image. The image content analysis system presented in this paper aims to provide the machine with the capability to simulate in some sense, a similar capability in human beings. The developed system consists of three levels. In the low level, image clustering is performed to extract features of the input data and to reduce dimensionality of the feature space. Classification of the scene images are carried out using a single layer neural network, trained through Kohonen's self-organizing algorithm, with conscience function, to produce a set of equi-probable weights vector. The intermediate level consists of two parts. In the first part an image is partitioned into homogeneous regions with respect to the connectivity property between pixels, which is an important concept used in establishing boundaries of objects and component regions in an image. For each component, connected components can be determined by a process of component labeling. In the second part, feature extraction process is performed to capture significant properties of objects present in the image. In the high level; extracted features and relations of each region in the image are matched against the stored object models using the genetic algorithm approach. The implemented system is used in the analysis and recognition of colored images that represent natural scenes. Keywords: genetic algorithms, neural networks, image segmentation, clustering, image content analysis

A spatially-structured PCG method for content diversity in a Physics-based simulation game

This paper presents a spatially-structured evolutionary algorithm (EA) to procedurally generate game maps of di ferent levels of di ficulty to be solved, in Gravityvolve!, a physics-based simulation videogame that we have implemented and which is inspired by the n- body problem, a classical problem in the fi eld of physics and mathematics. The proposal consists of a steady-state EA whose population is partitioned into three groups according to the di ficulty of the generated content (hard, medium or easy) which can be easily adapted to handle the automatic creation of content of diverse nature in other games. In addition, we present three fitness functions, based on multiple criteria (i.e:, intersections, gravitational acceleration and simulations), that were used experimentally to conduct the search process for creating a database of maps with di ferent di ficulty in Gravityvolve!.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Single shot cathode transverse momentum imaging in high brightness photoinjectors

In state of the art photoinjector electron sources, thermal emittance from photoemission dominates the final injector emittance. Therefore, low thermal emittance cathode developments and diagnostics are very important. Conventional thermal emittance measurements for the high gradient gun are time-consuming and thus thermal emittance is not measured as frequently as quantum efficiency during the lifetime of photocathodes, although both are important properties for the photoinjector optimizations. In this paper, a single shot measurement of photoemission transverse momentum, i.e., thermal emittance per rms laser spot size, is proposed for photocathode rf guns. By tuning the gun solenoid focusing, the electrons' transverse momenta at the cathode are imaged to a downstream screen, which enables a single shot measurement of both the rms value and the detailed spectra of the photoelectrons' transverse momenta. Both simulations and proof of principle experiments are reported

Upcycling Used Garments to Recreate Sustainable Fashion Designs Treated by Soil Release Finishing

Upcycled garments can have that independent appeal. No matter whom the designer is or what styling options designers choose, these upcycled fashion garments and accessories are by nature one-of-a kind. Finishing of textile fabric is carried out to increase attractiveness and/or serviceability of the fabric. Different finishing treatments are available to get various effects, which add value to the basic textile material, which can be better solutions for sustainable fashion. The sustainable fashion was achieved to a certain extent by using available materials to its ultimate USAge, using waste material, recycling of the products, planning second life for the fashion product, slowing down the fashion etc. The main idea to do so is to develop more sustainable and responsible designs. This paper is not just about recycled clothes but about fashion, recycling, upcycling, adding value and uniqueness by the resulting one-of-a-kind design available for women to purchase in any retail store. Create nine designs using waste garments treated by soil release finishing agent as a source of sustainable fashionable designs

Magnetic resonance imaging of the left wrist: assessment of the bone age in a sample of healthy Iraqi adolescent males

Background: Use of magnetic resonance imaging (MRI) to calculate skeletal age is a novel idea. MRI provides excellent soft-tissue contrast and multiplanar cross-sectional imaging capability. It could be used as an alternative method of skeletal age determination. Objectives: To study the value of MRI in estimating the age of healthy Iraqi adolescent males and to compare the obtained results with other countries records. Population and methods: This cross sectional study was applied on 179 healthy adolescent males between the ages of 13 to18 years in MRI unit at radiology institute in medical city, Baghdad – Iraq. This study was carried out from November 2011 to December 2012. Magnetic resonance imaging of the left wrist was performed by using a 1.5Tesla machine with surface coil. The sequence used was coronal T1weighted images (WI). The degree of fusion of the left distal radial physis was determined by a newly developed grading system. Results: There is high correlation between chronological age and degree of fusion of distal radius within the participant population. Most adolescent boys in the age group between 13 and 14 years presented as grade I and II, while the complete fusion was found at the age of 17and18 years, the mean age of participants was 17.5 years. Degree of fusion of the distal radius in the sample of the study was almost approaching the obtained values in the Algeria and Malaysia as comparative countries. Conclusion: MRI offers an alternative; non-invasive method of examination of the epiphysial fusion, which eliminates any risk associated with standard radiographic rating. The grading system can accurately identify the variable degrees of epiphysial fusion in an objective teachable manner

Investigation on the Effects of Carbon Nanotubes and Kinetin on Growth and Regeneration of Salvia nemorosa under in vitro Conditions

Introduction The use of medicinal plants has surged in recent times, with a substantial portion of modern medicines derived from botanical sources. This surge in demand underscores the potential of cultivating and producing medicinal plants to not only bolster public health but also significantly contribute to a society's economic well-being.Salvia nemorosa is an herbaceous, perennial, and medicinal plant. This valuable plant belongs to the Labiatae family. The aerial parts of the plant, especially the leaves, contain essential oils. Furthermore, S. nemorosa contains chemical compositions such as saponin, organic acids, diterpenes, triterpenes, polyphenols, and a bitter substance called picrosalvin. In the plant tissue culture, the preparation of culture medium and formulation compatible with the tissue of the tested plant is vital for efficient regeneration and plant growth. Generally, no specific culture medium can be recommended for the growth of explants, and is necessary to make changes in the culture medium in order to better respond to different types of explants. Carbon nanotubes are nanomaterials that can be added to the culture medium and increase the growth rate of different parts of the plant such as roots, stems, and branches. Cytokines also commonly participate in cell divisions and proliferate shoots by removing terminal dominance. Considering the mentioned importance, this study was conducted to investigate the effect of carbon nanotubes, kinetin, and their interactions on some morphophysiological and phytochemical properties of S. nemorosa under in vitro conditions.   Materials and Methods This experiment was carried out in the tissue culture and biotechnology laboratory of the Department of Horticultural Sciences, Faculty of Agriculture, University of Mohaghegh Ardabili. This study was conducted based on factorial design (completely randomized design) with five replications. The treatments included five levels of carbon nanotubes (0, 10, 20, 40, and 80 mg/L CNT) and three levels of kinetin (0, 2, and 4 mg/L CK). The seeds of S. nemorosa were sterilized with 70% ethanol for 40 seconds and then 2.5% hypochlorite sodium for 15 minutes (then the seed were washed with deionized water three times 3, 5, and 15 minutes). The sterilized seeds were planted in MS medium containing 30 g/L sucrose and 8 g/L agar, and then transferred to a growth chamber. After 40 days, the obtained seedlings were cut into single nodes by removing leaves and transferred to the main treatment medium. After that, some traits such as fresh weight, number of branches, number of leaves, number of roots, leaf area, root length, seedlings height, viability rate, germination rate, chlorophylls a, b, carotenoid content, phenol content, and flavonoid content were examined. The obtained data related to the experiment were analyzed with SAS software, the comparison of data means was done with Duncan's test, and the graphs were plotted using Excel software. Results and Discussion The results indicated that the interaction effect of carbon nanotubes and kinetin on the indices of fresh weight, number of branches, number of leaves, number of roots, leaf area, root length, seedling height, survival percentage, germination percentage, pigment content photosynthesis was not significant. On the contrary, the interaction effect of two treatments on the content of total flavonoid and total phenol showed a significant difference. The highest phenol content was obtained in the treatment combination of 80 mg/L of carbon nanotubes and 4 mg/L of kinetin. On the other hand, increasing the concentration of carbon nanotubes up to 80 mg/L, the average shoot production, number of leaves, leaf area, plant height, root number, root length, chlorophyll a, b, carotenoid, phenol and flavonoid content. increased significantly. Also, by increasing the concentration of kinetin (4 mg/liter), the number of branches, the content of chlorophyll a and b increased significantly. Conclusion The characteristics of the S. nemorosa plant, except for fresh weight, significantly increased under the influence of carbon nanotube treatments. However, under the influence of kinetin treatment, only the number of branches, chlorophyll a, b, phenol content, and flavonoid content showed a significant increase. Based on the results of this study, carbon nanotubes can be used for proliferation and increasing the secondary metabolites of S. nemorosa. Despite the results of this study, it is still possible to use higher concentrations of carbon nanotubes in future research in order to increase the phytochemical properties and productivity of other medicinal plants

Cognitive behaviour therapy versus counselling intervention for anxiety in young people with high-functioning autism spectrum disorders: a pilot randomised controlled trial

The use of cognitive-behavioural therapy (CBT) as a treatment for children and adolescents with autism spectrum disorder (ASD) has been explored in a number of trials. Whilst CBT appears superior to no treatment or treatment as usual, few studies have assessed CBT against a control group receiving an alternative therapy. Our randomised controlled trial compared use of CBT against person-centred counselling for anxiety in 36 young people with ASD, ages 12–18. Outcome measures included parent- teacher- and self-reports of anxiety and social disability. Whilst each therapy produced improvements inparticipants, neither therapy was superior to the other to a significant degree on any measure. This is consistent with findings for adults