3,029 research outputs found
tuppence-based SERS for the detection of illicit materials
Deposition of silver onto British 2p coins has been demonstrated as an
efficient and cost effective approach to producing substrates capable of
promoting surface enhanced Raman scattering (SERS). Silver application to the
copper coins is undemanding taking just 20 s, and results in the formation of
multiple hierarchial dendritic structures. To demonstrate that the silver
deposition sites were capable of SERS the highly fluorescent Rhodamine 6G
(R6G) probe was used. Analyses indicated that Raman enhancement only occurs at
the silver deposition sites and not from the roughened copper surface. The
robustness of the substrate in the identification and discrimination of
illegal and legal drugs of abuse was then explored. Application of the drugs
to the substrates was carried out using spotting and soaking methodologies.
Whilst little or no SERS spectra of the drugs were generated upon spotting,
soaking of the substrate in a methanolic solution of the drugs yielded a vast
amount of spectral information. Excellent reproducibility of the SERS method
and classification of three of the drugs, 4-methylmethcathinone (mephedrone),
5,6-methylenedioxy-2-aminoindane (MDAI) and
3,4-methylenedioxy-N-methylamphetamine (MDMA) were demonstrated using
principal components analysis and partial least squares
Dynamics of Triangulations
We study a few problems related to Markov processes of flipping
triangulations of the sphere. We show that these processes are ergodic and
mixing, but find a natural example which does not satisfy detailed balance. In
this example, the expected distribution of the degrees of the nodes seems to
follow the power law
The Definition and Measurement of the Topological Entropy per Unit Volume in Parabolic PDE's
We define the topological entropy per unit volume in parabolic PDE's such as
the complex Ginzburg-Landau equation, and show that it exists, and is bounded
by the upper Hausdorff dimension times the maximal expansion rate. We then give
a constructive implementation of a bound on the inertial range of such
equations. Using this bound, we are able to propose a finite sampling algorithm
which allows (in principle) to measure this entropy from experimental data.Comment: 26 pages, 1 small figur
Heteroclinic Chaos, Chaotic Itinerancy and Neutral Attractors in Symmetrical Replicator Equations with Mutations
A replicator equation with mutation processes is numerically studied.
Without any mutations, two characteristics of the replicator dynamics are
known: an exponential divergence of the dominance period, and hierarchical
orderings of the attractors. A mutation introduces some new aspects: the
emergence of structurally stable attractors, and chaotic itinerant behavior. In
addition, it is reported that a neutral attractor can exist in the mutataion
rate -> +0 region.Comment: 4 pages, 9 figure
Antimicrobial Treatmdent of "Complicated" Intra-Abdominal Infections and The New IDSA Guidelines - A Commentary and an Alternative European Approach According to Clinical Definitions
Recently, an update of the IDSA guidelines for the treatment of complicated intraabdominal infections has been published. No guideline can cater for all variations in ecology, antimicrobial resistance patterns, patient characteristics and presentation, health care and reimbursement systems in many different countries. In the short time the IDSA guidelines have been available, a number of practical clinical issues have been raised by physicians regarding interpretation of the guidelines. The main debatable issues of the new IDSA guidelines are described as follows
Activin and TGFβ use diverging mitogenic signaling in advanced colon cancer.
BackgroundUnderstanding cell signaling pathways that contribute to metastatic colon cancer is critical to risk stratification in the era of personalized therapeutics. Here, we dissect the unique involvement of mitogenic pathways in a TGFβ or activin-induced metastatic phenotype of colon cancer.MethodMitogenic signaling/growth factor receptor status and p21 localization were correlated in primary colon cancers and intestinal tumors from either AOM/DSS treated ACVR2A (activin receptor 2) -/- or wild type mice. Colon cancer cell lines (+/- SMAD4) were interrogated for ligand-induced PI3K and MEK/ERK pathway activation and downstream protein/phospho-isoform expression/association after knockdown and pharmacologic inhibition of pathway members. EMT was assessed using epithelial/mesenchymal markers and migration assays.ResultsIn primary colon cancers, loss of nuclear p21 correlated with upstream activation of activin/PI3K while nuclear p21 expression was associated with TGFβ/MEK/ERK pathway activation. Activin, but not TGFβ, led to PI3K activation via interaction of ACVR1B and p85 independent of SMAD4, resulting in p21 downregulation. In contrast, TGFβ increased p21 via MEK/ERK pathway through a SMAD4-dependent mechanism. While activin induced EMT via PI3K, TGFβ induced EMT via MEK/ERK activation. In vivo, loss of ACVR2A resulted in loss of pAkt, consistent with activin-dependent PI3K signaling.ConclusionAlthough activin and TGFβ share growth suppressive SMAD signaling in colon cancer, they diverge in their SMAD4-independent pro-migratory signaling utilizing distinct mitogenic signaling pathways that affect EMT. p21 localization in colon cancer may determine a dominant activin versus TGFβ ligand signaling phenotype warranting further validation as a therapeutic biomarker prior to targeting TGFβ family receptors
Remarks on Bootstrap Percolation in Metric Networks
We examine bootstrap percolation in d-dimensional, directed metric graphs in
the context of recent measurements of firing dynamics in 2D neuronal cultures.
There are two regimes, depending on the graph size N. Large metric graphs are
ignited by the occurrence of critical nuclei, which initially occupy an
infinitesimal fraction, f_* -> 0, of the graph and then explode throughout a
finite fraction. Smaller metric graphs are effectively random in the sense that
their ignition requires the initial ignition of a finite, unlocalized fraction
of the graph, f_* >0. The crossover between the two regimes is at a size N_*
which scales exponentially with the connectivity range \lambda like_* \sim
\exp\lambda^d. The neuronal cultures are finite metric graphs of size N \simeq
10^5-10^6, which, for the parameters of the experiment, is effectively random
since N<< N_*. This explains the seeming contradiction in the observed finite
f_* in these cultures. Finally, we discuss the dynamics of the firing front
Large deviations of lattice Hamiltonian dynamics coupled to stochastic thermostats
We discuss the Donsker-Varadhan theory of large deviations in the framework
of Hamiltonian systems thermostated by a Gaussian stochastic coupling. We
derive a general formula for the Donsker-Varadhan large deviation functional
for dynamics which satisfy natural properties under time reversal. Next, we
discuss the characterization of the stationary state as the solution of a
variational principle and its relation to the minimum entropy production
principle. Finally, we compute the large deviation functional of the current in
the case of a harmonic chain thermostated by a Gaussian stochastic coupling.Comment: Revised version, published in Journal of Statistical Physic
- …