Characterization of four cell lines persistently infected with measles virus

Persistently infected cell lines were established by infecting Vero cells with four different strains of measles virus: Edmonston “wild type”, Schwarz vaccine strain passaged at high multiplicity of infection, Hallé SSPE strain, and a temperature sensitive mutant of Edmonston strain, designated ts 841. The four cell lines have continued to produce virus at a constant low level over a period of more than two years, although cytopathology and hemagglutinating ability have varied with cell passage. Only virus from cells originally infected with ts 841 appears to be temperature sensitive. In each of the cell lines a sizable population of low density, interfering virus particles was generated, indicating that this is an important mechanism for these four cell lines in maintenance of the measles virus persistent infection.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41690/1/705_2005_Article_BF01314863.pd

Enhancement and suppression by actinomycin D of a Vero cell nontransmissible measles infection

The effect of low doses of actinomycin D on Vero cell nontransmissible measles infection produced after cocultivation with a HeLa subline with persistent defective infection by Edmonston measles virus was examined by pretreatment of Vero cells with the drug and treatment at 1, 4, and 7 days after Vero cell infection. Pretreatment enhanced focal formation of viral immunofluorescent Vero cell syncytia but did not induce synthesis of detectable amounts od cocultures suppressed syncytial formation, and treatment of 4- and 7-day-old cocultures had little, if any, effect on syncytial formation. Pretreatment also eliminated a transient resistance to homologous superinfection. A possible relation of these findings to the presence of a cell-associated viral inhibitor in the persistently infected HeLa cells is discussed.</jats:p