Photosynthesis dependent acidification of perialgal vacuoles in theParamedum bursaria/Chlorella symbiosis. Visualization by monensin

After treatment with the carboxylic ionophore monensin theChlorella containing perialgal vacuoles of the greenParamecium bursaria swell. TheParamecium cells remain motile at this concentration for at least one day. The swelling is only observed in illuminated cells and can be inhibited by DCMU. We assume that during photosynthesis the perialgal vacuoles are acidified and that monensin exchanges H+ ions against monovalent cations (here K+). In consequence the osmotic value of the vacuoles increases. The proton gradient is believed to drive the transport of maltose from the symbiont into the host. Another but light independent effect of the monensin treatment is the swelling of peripheral alveoles of the ciliates, likewise indicating that the alveolar membrane contains an active proton pump

Atlas climático da Região Sul do Brasil: Estados do Paraná, Santa Catarina e Rio Grande do Sul.

Este atlas é dividido em quatro principais seções, sendo a primeira a descrição conceitual e metodológica das variáveis meteorológicas. A segunda seção compreende uma análise, por meio de gráficos, das principais características climáticas dos mais diversos ambientes regionais. Em seguida, estão disponíveis, na forma de tabelas, os dados mensais, estacionais e anuais que podem ser usados para outros fins, conforme a necessidade do leitor. Por último, ele apresenta mais de duas centenas de mapas que revelam, de forma ilustrada e de fácil visualização, um conjunto de variáveis de temperatura, precipitação pluvial, classificações climáticas, entre outras informações.bitstream/item/143521/1/Atlas-climatico-da-regiao-Sul-do-Brasil.pdf2ª ediçã

Caracterízação de uvas finas de mesa produzidas em ambiente protegido.

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Identification of general patterns of sex-biased expression in Daphnia, a genus with environmental sex determination

Daphnia reproduce by cyclic-parthenogenesis, where phases of asexual reproduction are intermitted by sexual production of diapause stages. This life cycle, together with environmental sex determination, allow the comparison of gene expression between genetically identical males and females. We investigated gene expression differences between males and females in four genotypes of Daphnia magna and compared the results with published data on sex-biased gene expression in two other Daphnia species, each representing one of the major phylogenetic clades within the genus. We found that 42% of all annotated genes showed sex-biased expression in D. magna. This proportion is similar both to estimates from other Daphnia species as well as from species with genetic sex determination, suggesting that sex-biased expression is not reduced under environmental sex determination. Among 7453 single copy, one-to-one orthologs in the three Daphnia species, 707 consistently showed sex-biased expression and 675 were biased in the same direction in all three species. Hence these genes represent a core-set of genes with consistent sex-differential expression in the genus. A functional analysis identified that several of them are involved in known sex determination pathways. Moreover, 75% were overexpressed in females rather than males, a pattern that appears to be a general feature of sex-biased gene expression in Daphnia

Variações da temperatura do ar e do solo sob a influência de filmes plásticos de diferentes cores na produção do morangueiro.

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Comportamento produtivo de novas cultivares de morangueiro na região de Pelotas, RS.

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