Identificação de polinizadores na progênie da matriz de erva-mate cambona-4, usando marcadores RAPD.

O presente trabalho teve como objetivo determinar a paternidade de uma progênie de erva-mate da matriz Cambona-4 e quatro possíveis polinizadores (A,B,C e D). O mesmo visa a produção de mudas por sementes com a manutenção das características agronômicas e principalmente suavidade no sabor, inerentes à matriz Cambona-4. Para tanto, foram utilizados marcadores RAPD identificando-se fragmentos presentes em apenas um dos polinizadores e na progênie e, obrigatoriamente, ausentes nos demais polinizadores e na matriz. Um mínimo de seis fragmentos com estas características foram usados para determinação de cada paternidade. Obteve-se a confirmação de 83,3% de filhos oriundos do cruzamento com o polinizador A, 11,9% com o polinizador B, 4,8% com o polinizador C. Nenhuma planta da progênie confirmou ser filha do cruzamento com o polinizador D. De um total de 107 progênies testadas, 23 não foram possíveis de determinar sua paternidade com os primers utilizados. A determinação da paternidade preferencialmente pelo polinizador A permitirá o direcionamento de cruzamentos a fim de obter-se sementes e mudas com uma variabilidade baixa e características próximas à da matriz Cambona-4, uma vez que as plantas da progênie confirmadas como Cambona-4/polinizador A apresentaram características próximas à planta matriz, com 90% das plantas semelhantes quanto à cor da folha e 84% quanto ao brilho da folha. Testes preliminares de degustação mostram que a característica de suavidade no sabor está mantida nesta progênie, embora ensaios sensoriais estejam em fase de programação.Secão: Conservação, Melhoramento e Multiplicação. Feira do Agronegócio da Erva-mate, 1., 2003, Chapecó. Integrar para promover o agronegócio da erva-mate

Ground truth annotation of traffic video data

This paper presents a software application to generate ground-truth data on video files from traffic surveillance cameras used for Intelligent Transportation Systems (IT systems). The computer vision system to be evaluated counts the number of vehicles that cross a line per time unit intensity-, the average speed and the occupancy. The main goal of the visual interface presented in this paper is to be easy to use without the requirement of any specific hardware. It is based on a standard laptop or desktop computer and a Jog shuttle wheel. The setup is efficient and comfortable because one hand of the annotating person is almost all the time on the space key of the keyboard while the other hand is on the jog shuttle wheel. The mean time required to annotate a video file ranges from 1 to 5 times its duration (per lane) depending on the content. Compared to general purpose annotation tool a time factor gain of about 7 times is achieved.This work was funded by the Spanish Government project MARTA under the CENIT program and CICYT contract TEC2009-09146.Mossi García, JM.; Albiol Colomer, AJ.; Albiol Colomer, A.; Oliver Moll, J. (2014). Ground truth annotation of traffic video data. Multimedia Tools and Applications. 1-14. https://doi.org/10.1007/s11042-013-1396-xS114Albiol A et al (2011) Detection of parked vehicles using spatiotemporal maps. IEEE Trans Intell Transport Syst 12(4):1277–1291Blunsden SJ, Fisher R (2010) The BEHAVE video dataset: ground truthed video for multi-person behavior classification. Annal British Mach Vis Assoc 4:1–12Bradski G, Kaehler A (2008) Learning OpenCV: Computer vision with the OpenCV library. O'Reilly Media, IncorporatedBrooke J. SUS: a “quick and dirty” usability scale. Usability evaluation in industry. Taylor and FrancisBrostow GJ et al (2009) Semantic object classes in video: a high-definition ground truth database. Pattern Recognit Lett 30(2):88–97Buch N et al (2011) A review of computer vision techniques for the analysis of urban traffic. IEEE Trans Intell Transp Syst 12(3):920–939D’Orazio T et al. (2009) A semi-automatic system for ground truth generation of soccer video sequences. Advanced Video and Signal Based Surveillance, 2009. AVSS’09. Sixth IEEE International Conference on (Sep. 2009), 559–564Dollar P et al (2012) Pedestrian detection: an evaluation of the state of the art. IEEE Trans Pattern Anal Mach Intell 34(4):743–761Faro A et al (2011) Adaptive background modeling integrated with luminosity sensors and occlusion processing for reliable vehicle detection. IEEE Trans Intell Transport Syst 12(4):1398–1412Giro-i-Nieto X et al (2010) GAT: a graphical annotation tool for semantic regions. Multimed Tool Appl 46(2–3):155–174i-LIDS. Image Library for Intelligent Detection Systems: www.ilids.co.uk . Home Office Scientific Development Branch, United Kingdom. Last Accessed February 2013Kasturi R et al (2009) Framework for performance evaluation of face, text, and vehicle detection and tracking in video: data, metrics, and protocol. IEEE Trans Pattern Anal Mach Intell 31(2):319–336Laganière R (2011) OpenCV 2 computer vision application programming cookbook. Packt Pub LimitedLorist MM et al (2000) Mental fatigue and task control: planning and preparation. Psychophysiology 37(5):614–625Russell B et al (2008) LabelMe: a database and web-based tool for image annotation. Int J Comput Vis 77(1):157–173Serrano M, Gracía J, Patricio M, Molina J (2010). Interactive video annotation tool. Distributed Computing and Artificial Intelligence, 325–332Traffic City Cameras. Ajuntament de València, Spain. http://camaras.valencia.es . Last Accessed February 2013TREC video retrieval evaluation. http://www-nlpir.nist.gov/projects/trecvid/Vezzani R, Cucchiara R (2010) Video Surveillance Online Repository (ViSOR): an integrated framework. Multimed Tool Appl 50(2):359–380ViPER: the video performance evaluation resource: http://viper-toolkit.sourceforge.net/Volkmer T et al. (2005) A web-based system for collaborative annotation of large image and video collections: an evaluation and user study. Proceedings of the 13th annual ACM international conference on Multimedia (New York, NY, USA, 2005), 892–901Zhang HB, Li SA, Chen SY, Su SZ, Duh DJ, Li SZ (2012) Adaptive photograph retrieval method. Multimedia Tools and Applications, Published online September 2012.Zou Y et al (2011) Traffic incident classification at intersections based on image sequences by HMM/SVM classifiers. Multimed Tool Appl 52(1):133–14

Odour-mediated orientation of beetles is influenced by age, sex and morph

The behaviour of insects is dictated by a combination of factors and may vary considerably between individuals, but small insects are often considered en masse and thus these differences can be overlooked. For example, the cowpea bruchid Callosobruchus maculatus F. exists naturally in two adult forms: the active (flight) form for dispersal, and the inactive (flightless), more fecund but shorter-lived form. Given that these morphs show dissimilar biology, it is possible that they differ in odour-mediated orientation and yet studies of this species frequently neglect to distinguish morph type, or are carried out only on the inactive morph. Along with sex and age of individual, adult morph could be an important variable determining the biology of this and similar species, informing studies on evolution, ecology and pest management. We used an olfactometer with motion-tracking to investigate whether the olfactory behaviour and orientation of C. maculatus towards infested and uninfested cowpeas and a plant-derived repellent compound, methyl salicylate, differed between morphs or sexes. We found significant differences between the behaviour of male and female beetles and beetles of different ages, as well as interactive effects of sex, morph and age, in response to both host and repellent odours. This study demonstrates that behavioural experiments on insects should control for sex and age, while also considering differences between adult morphs where present in insect species. This finding has broad implications for fundamental entomological research, particularly when exploring the relationships between physiology, behaviour and evolutionary biology, and the application of crop protection strategies

CNNs for automatic glaucoma assessment using fundus images: an extensive validation

Background Most current algorithms for automatic glaucoma assessment using fundus images rely on handcrafted features based on segmentation, which are affected by the performance of the chosen segmentation method and the extracted features. Among other characteristics, convolutional neural networks (CNNs) are known because of their ability to learn highly discriminative features from raw pixel intensities. Methods In this paper, we employed five different ImageNet-trained models (VGG16, VGG19, InceptionV3, ResNet50 and Xception) for automatic glaucoma assessment using fundus images. Results from an extensive validation using cross-validation and cross-testing strategies were compared with previous works in the literature. Results Using five public databases (1707 images), an average AUC of 0.9605 with a 95% confidence interval of 95.92–97.07%, an average specificity of 0.8580 and an average sensitivity of 0.9346 were obtained after using the Xception architecture, significantly improving the performance of other state-of-the-art works. Moreover, a new clinical database, ACRIMA, has been made publicly available, containing 705 labelled images. It is composed of 396 glaucomatous images and 309 normal images, which means, the largest public database for glaucoma diagnosis. The high specificity and sensitivity obtained from the proposed approach are supported by an extensive validation using not only the cross-validation strategy but also the cross-testing validation on, to the best of the authors’ knowledge, all publicly available glaucoma-labelled databases. Conclusions These results suggest that using ImageNet-trained models is a robust alternative for automatic glaucoma screening system. All images, CNN weights and software used to fine-tune and test the five CNNs are publicly available, which could be used as a testbed for further comparisons

A Major Role for the Plasmodium falciparum ApiAP2 Protein PfSIP2 in Chromosome End Biology

The heterochromatic environment and physical clustering of chromosome ends at the nuclear periphery provide a functional and structural framework for antigenic variation and evolution of subtelomeric virulence gene families in the malaria parasite Plasmodium falciparum. While recent studies assigned important roles for reversible histone modifications, silent information regulator 2 and heterochromatin protein 1 (PfHP1) in epigenetic control of variegated expression, factors involved in the recruitment and organization of subtelomeric heterochromatin remain unknown. Here, we describe the purification and characterization of PfSIP2, a member of the ApiAP2 family of putative transcription factors, as the unknown nuclear factor interacting specifically with cis-acting SPE2 motif arrays in subtelomeric domains. Interestingly, SPE2 is not bound by the full-length protein but rather by a 60kDa N-terminal domain, PfSIP2-N, which is released during schizogony. Our experimental re-definition of the SPE2/PfSIP2-N interaction highlights the strict requirement of both adjacent AP2 domains and a conserved bipartite SPE2 consensus motif for high-affinity binding. Genome-wide in silico mapping identified 777 putative binding sites, 94% of which cluster in heterochromatic domains upstream of subtelomeric var genes and in telomere-associated repeat elements. Immunofluorescence and chromatin immunoprecipitation (ChIP) assays revealed co-localization of PfSIP2-N with PfHP1 at chromosome ends. Genome-wide ChIP demonstrated the exclusive binding of PfSIP2-N to subtelomeric SPE2 landmarks in vivo but not to single chromosome-internal sites. Consistent with this specialized distribution pattern, PfSIP2-N over-expression has no effect on global gene transcription. Hence, contrary to the previously proposed role for this factor in gene activation, our results provide strong evidence for the first time for the involvement of an ApiAP2 factor in heterochromatin formation and genome integrity. These findings are highly relevant for our understanding of chromosome end biology and variegated expression in P. falciparum and other eukaryotes, and for the future analysis of the role of ApiAP2-DNA interactions in parasite biology