COMPUTER AIDED DESIGN IN WHEY PROCESSING

\Vhey processing alternatives were investigated with the help of Computer Aided Design. Mass and energy balances were calculated, process units were sized, and an economic analysis vas conducted. Using IRR and ACF methods for calculating profitability methods optimum economic strategies were suggested for whey processing

Bitwise Reproducible task execution on unstructured mesh applications

Many mesh applications use floating point arithmetic which do not necessarily hold the associative laws of algebra. This could cause the application to become unreproducible. In this paper we present some work on generating a method for unstructured mesh applications to provide bitwise reproducibility between separate runs, even if they are started with different number of MPI processes. We implement our work in the OP2 domain-specific library, which provides an API that abstracts the solution of unstructured mesh computations. We carry out a performance analysis of our method applied on two applications: a simple airfoil application, and a more complex Aero application which uses a finite element method and a conjugate-gradient algorithm. We show a 2.37×to 1.49× slowdown on this applications as a price for full bitwise reproducibility

Low-mutation-rate, reduced-genome Escherichia coli: an improved host for faithful maintenance of engineered genetic constructs

<p>Abstract</p> <p>Background</p> <p>Molecular mechanisms generating genetic variation provide the basis for evolution and long-term survival of a population in a changing environment. In stable, laboratory conditions, the variation-generating mechanisms are dispensable, as there is limited need for the cell to adapt to adverse conditions. In fact, newly emerging, evolved features might be undesirable when working on highly refined, precise molecular and synthetic biological tasks.</p> <p>Results</p> <p>By constructing low-mutation-rate variants, we reduced the evolutionary capacity of MDS42, a reduced-genome <it>E. coli </it>strain engineered to lack most genes irrelevant for laboratory/industrial applications. Elimination of diversity-generating, error-prone DNA polymerase enzymes involved in induced mutagenesis achieved a significant stabilization of the genome. The resulting strain, while retaining normal growth, showed a significant decrease in overall mutation rates, most notably under various stress conditions. Moreover, the error-prone polymerase-free host allowed relatively stable maintenance of a toxic methyltransferase-expressing clone. In contrast, the parental strain produced mutant clones, unable to produce functional methyltransferase, which quickly overgrew the culture to a high ratio (50% of clones in a 24-h induction period lacked functional methyltransferase activity). The surprisingly large stability-difference observed between the strains was due to the combined effects of high stress-induced mutagenesis in the parental strain, growth inhibition by expression of the toxic protein, and selection/outgrowth of mutants no longer producing an active, toxic enzyme.</p> <p>Conclusions</p> <p>By eliminating stress-inducible error-prone DNA-polymerases, the genome of the mobile genetic element-free <it>E. coli </it>strain MDS42 was further stabilized. The resulting strain represents an improved host in various synthetic and molecular biological applications, allowing more stable production of growth-inhibiting biomolecules.</p

Chaos and stability in a two-parameter family of convex billiard tables

We study, by numerical simulations and semi-rigorous arguments, a two-parameter family of convex, two-dimensional billiard tables, generalizing the one-parameter class of oval billiards of Benettin--Strelcyn [Phys. Rev. A 17, 773 (1978)]. We observe interesting dynamical phenomena when the billiard tables are continuously deformed from the integrable circular billiard to different versions of completely-chaotic stadia. In particular, we conjecture that a new class of ergodic billiard tables is obtained in certain regions of the two-dimensional parameter space, when the billiards are close to skewed stadia. We provide heuristic arguments supporting this conjecture, and give numerical confirmation using the powerful method of Lyapunov-weighted dynamics.Comment: 19 pages, 13 figures. Submitted for publication. Supplementary video available at http://sistemas.fciencias.unam.mx/~dsanders

Microwave-assisted synthesis of α-aminophosphonates with sterically demanding α-aryl substituents

A series of N-benzhydryl protected α-aminophosphonates with α-phenyl, α-(1-naphtyl), α-(9-anthryl) or α-(1-pyrenyl) substituents was synthesized by the Kabachnik–Fields condensation of diphenylmethylamine (benzhydrylamine), the corresponding aryl aldehyde and a dialkyl phosphite under MW irradiation. X-ray studies performed at low temperatures for a few of these α-aminophosphonates confirmed the presence of unusually short intramolecular Cα–Hδ+ ··· δ+H–Cperi contacts

Approximating multi-dimensional Hamiltonian flows by billiards

Consider a family of smooth potentials $V_{\epsilon}$, which, in the limit $\epsilon\to0$, become a singular hard-wall potential of a multi-dimensional billiard. We define auxiliary billiard domains that asymptote, as $\epsilon\to0$ to the original billiard, and provide asymptotic expansion of the smooth Hamiltonian solution in terms of these billiard approximations. The asymptotic expansion includes error estimates in the $C^{r}$ norm and an iteration scheme for improving this approximation. Applying this theory to smooth potentials which limit to the multi-dimensional close to ellipsoidal billiards, we predict when the separatrix splitting persists for various types of potentials

STAT2/IRF9 directs a prolonged ISGF3-like transcriptional response and antiviral activity in the absence of STAT1

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