Lossy mode resonance enabling ultra-low detection limit for fibre-optic biosensors (INVITED)

The combination of optical fibre-based biosensors with nanotechnologies is providing the opportunity for the development of in situ, portable, lightweight, versatile and high-sensitivity optical sensing platforms. We report on the generation of lossy mode resonances (LMRs) by means of the deposition of nm-thick SnO2 film on optical fibres. This allows measuring precisely and accurately the changes in refractive index of the fibre-surrounding medium with very high sensitivity compared to other optical technology platforms, such as long period grating or surface plasmon resonance. This approach, mixed with the use of specialty fiber structures such as Dshaped fibres, allows improving the light-matter interaction in strong way. Different imaging systems, i.e. SEM and TEM along with X-EDS tool, have been used to study the optical features of the fiber coating. The shift of the LMR has been monitored in real-time thanks to conventional wavelength interrogation system and ad hoc developed microfluidics. A big leap in performance has been attained by detecting femtomolar concentrations in human serum. The biosensor reusability has been also tested by using a solution of sodium dodecyl sulphate.This work was supported by the National Research Council of Italy (CNR) for the Short Term Mobility program 2017, by the Government of Navarra (project no. 72/2015) and by the Spanish Agencia Estatal de Investigacion (AEI) and European Regional Development Fund (FEDER) (TEC2016-78047-R,TEC2016-79367-C2-2-R)

Bioactive pectic polysaccharides from bay tree pruning waste: Sequential subcritical water extraction and application in active food packaging

The potential isolation of bio-active polysaccharides from bay tree pruning waste was studied using sequential subcritical water extraction using different time-temperature combinations. The extracted polysaccharides were highly enriched in pectins while preserving their high molecular mass (10–100 kDa), presenting ideal properties for its application as additive in food packaging. Pectin-enriched chitosan films were prepared, improving the optical properties (=95% UV-light barrier capacity), antioxidant capacity (?95% radical scavenging activity) and water vapor permeability (=14 g·Pa-1·s-1·m-1·10-7) in comparison with neat chitosan-based films. Furthermore, the antimicrobial activity of chitosan was maintained in the hybrid films. Addition of 10% of pectins improved mechanical properties, increasing the Young's modulus 12%, and the stress resistance in 51%. The application of pectin-rich fractions from bay tree pruning waste as an additive in active food packaging applications, with triple action as antioxidant, barrier, and antimicrobial has been demonstrated.Authors would like to thank the Spanish Ministry of Science and Innovation (Ramon y Cajal contract RYC-2015-17109) and Universidad de Cordoba, ´ Spain (Predoctoral Grant 2019) for the financial support during this work

MODASC: ASIC for mobile data acquisition systems using satellite communications

MODASC is an ASIC that performs wide area mobile data acquisition using satellite communication to provide global coverage. This circuit provides two operation modes: an autonomous mode, that periodically establishes connection with the control center, and a slave mode, working as a peripheral connected to a general purpose micro controller. This experiment has been realized under FUSE special action in collaboration with SAINSEL

Label-free fiber optic optrode for the detection of class C beta-lactamases expressed by drug resistant bacteria

This paper reports the experimental assessment of an automated optical assay based on label free optical fiber optrodes for the fast detection of class C beta-lactamases (AmpC BLs), actually considered as one of the most important sources of resistance to beta-lactams antibiotics expressed by resistant bacteria. Reflection-type long period fiber gratings (RT-LPG) have been used as highly sensitive label free optrodes, while a higher affine boronic acid based ligand was here selected to enhance the overall assay performances compared to those obtained in our first demonstration. In order to prove the feasibility analysis towards a fully automated optical assay, an engineered system was developed to simultaneously manipulate and interrogate multiple fiber optic optrodes in the different phases of the assay. The automated system tested in AmpC solutions at increasing concentrations demonstrated a limit of detection (LOD) of 6 nM, three times better when compared with the results obtained in our previous work. Moreover, the real effectiveness of the proposed optical assay has been also confirmed in complex matrices as the case of lysates of Escherichia coli overexpressing AmpC. (C) 2017 Optical Society of Americ

Effect of Grazing Management on Herbage Accumulation of Lucerne-Orchard Grass Sward

Throughout most of México, lucerne (Medicago sativa) is the primary forage legume used in the dairy industry. Unfortunately, lucerne does not grow in late autumn and winter due to adverse weather. Recent studies with lucerne have suggested that the inclusion of a companion grass will invariably increase the seasonal distribution and total annual yield of swards (Laidlaw & Teuber, 2001). However the management of mixed swards containing lucerne is difficult as a grazing frequency or intensity which suits one species may be detrimental to the other. Changes in balance between grass and legume, especially in grazed swards, have been observed. In México mixtures of lucerne-orchard grass have a good persistence and productivity. However the explanation for this is unclear. This study examined the effects of different grazing management practices on lucerne-orchard grass production and seasonal distribution

Non-linear effects of drought under shade: reconciling physiological and ecological models in plant communities

The combined effects of shade and drought on plant performance and the implications for species interactions are highly debated in plant ecology. Empirical evidence for positive and negative effects of shade on the performance of plants under dry conditions supports two contrasting theoretical models about the role of shade under dry conditions: the trade-off and the facilitation hypotheses. We performed a meta-analysis of field and greenhouse studies evaluating the effects of drought at two or more irradiance levels on nine response variables describing plant physiological condition, growth, and survival. We explored differences in plant response across plant functional types, ecosystem types and methodological approaches. The data were best fit using quadratic models indicating a humped-back shape response to drought along an irradiance gradient for survival, whole plant biomass, maximum photosynthetic capacity, stomatal conductance and maximal photochemical efficiency. Drought effects were ameliorated at intermediate irradiance, becoming more severe at higher or lower light levels. This general pattern was maintained when controlling for potential variations in the strength of the drought treatment among light levels. Our quantitative meta-analysis indicates that dense shade ameliorates drought especially among drought-intolerant and shade-tolerant species. Wet tropical species showed larger negative effects of drought with increasing irradiance than semiarid and cold temperate species. Non-linear responses to irradiance were stronger under field conditions than under controlled greenhouse conditions. Non-linear responses to drought along the irradiance gradient reconciliate opposing views in plant ecology, indicating that facilitation is more likely within certain range of environmental conditions, fading under deep shade, especially for drought-tolerant species

Reciclado de escorias de fondo de central térmica para su uso como áridos en la elaboración de componentes prefabricados de hormigón

The need to eliminate waste generates costs. When considering the preservation of the environment, the minimization of the consumption of natural resources and energy savings criteria, the need and advisability of studying the feasibility of waste re-use seems clear. However, waste re-use depends on whether they are economically competitive. Therefore, the aim of this study is to evaluate the possible use of slag from a steam power station as aggregate in the manufacture of concrete. This study included the determination of the physical, chemical and thermal properties of the material, comparing the results to those required by the Spanish structural concrete code (EHE) in determining their acceptance or rejection as a concrete component. The ultimate aim of the research was to determine the highest slag content that could be added to concrete without modifying its strength or durability, with a view to obtaining savings in the manufacture of precast structures.La necesidad de eliminar residuos genera gastos. Considerando criterios de conservación ambiental, minimización del consumo de recursos naturales y ahorro de energía parece claro la necesidad y conveniencia de estudiar la viabilidad del uso de residuos. Sin embargo la utilización de residuos depende de que sean competitivos económicamente. Por tanto el propósito de esta investigación es evaluar el posible uso de las escorias de fondo de una central térmica como áridos para la fabricación de hormigón. En este estudio se incluye la determinación de características físicas, químicas y térmicas y se han comparado los resultados a los requeridos por la EHE para determinar su aceptación o rechazo como componente de un hormigón. El objetivo final de la investigación responde a la utilización de hormigón con el máximo contenido en escorias sin modificar las condiciones de resistencia y durabilidad, consiguiendo un ahorro económico en la fabricación de estructuras prefabricadas

Intra-islet insulin synthesis defects are associated with endoplasmic reticulum stress and loss of beta cell identity in human diabetes

Aims/hypothesis: Endoplasmic reticulum (ER) stress and beta cell dedifferentiation both play leading roles in impaired insulin secretion in overt type 2 diabetes. Whether and how these factors are related in the natural history of the disease remains, however, unclear. Methods: In this study, we analysed pancreas biopsies from a cohort of metabolically characterised living donors to identify defects in in situ insulin synthesis and intra-islet expression of ER stress and beta cell phenotype markers. Results: We provide evidence that in situ altered insulin processing is closely connected to in vivo worsening of beta cell function. Further, activation of ER stress genes reflects the alteration of insulin processing in situ. Using a combination of 17 different markers, we characterised individual pancreatic islets from normal glucose tolerant, impaired glucose tolerant and type 2 diabetic participants and reconstructed disease progression. Conclusions/interpretation: Our study suggests that increased beta cell workload is accompanied by a progressive increase in ER stress with defects in insulin synthesis and loss of beta cell identity. Graphical abstract: [Figure not available: see fulltext.