Circular RNA Expression Profiling Identifies Prostate Cancer- Specific circRNAs in Prostate Cancer

Background/Aims: Prostate cancer (PCa) is one of the main cancers that damage males’ health severely with high morbidity and mortality, but there is still no ideal molecular marker for the diagnosis and prognosis of prostate cancer. Methods: To determine whether the differentially expressed circRNAs in prostate cancer can serve as novel biomarkers for prostate cancer diagnosis, we screened differentially expressed circRNAs using SBC-ceRNA array in 4 pairs of prostate tumor and paracancerous tissues. A circRNA-miRNA-mRNA regulatory network for the differential circRNAs and their host genes was constructed by Cytoscape3.5.1 software. Quantitative real-time polymerase chain reaction analysis (qRT-PCR) was performed to confirm the microarray data. Results: We found 1021 differentially expressed circRNAs in PCa tumor using SBC-ceRNA array and confirmed the expression of circ_0057558, circ_0062019 and SLC19A1 in PCa cell lines and tumor tissues through qRT-PCR analysis. We demonstrated that combination of PSA level and two differentially expressed circRNAs showed significantly increased AUC, sensitivity and specificity (0.938, 84.5% and 90.9%, respectively) than PSA alone (AUC of serum PSA was 0.854). Moreover, circ_0057558 was correlated positively with total cholesterol. The functional network of circRNA-miRNA-mRNA analysis showed that circ_0057558 and circ_0034467 regulated miR-6884, and circ_0062019 and circ_0060325 regulated miR-5008. Conclusion: Our results demonstrated that differentially expressed circRNAs (circ_0062019 and circ_0057558) and host gene SLC19A1 of circ_0062019 could be used as potential novel biomarkers for prostate cancer

How Abusive Supervision Affects Employees’ Unethical Behaviors: A Moderated Mediation Examination of Turnover Intentions and Caring Climate

Drawing on psychological contract theory, this research contributes to the unethical behavior literature by exploring employees’ turnover intentions as a mediator of the relationship between abusive supervision and employees’ unethical behavior and the moderating role of the caring climate in the relationship between turnover intentions and unethical behavior. The results from a sample of 679 reveal that turnover intentions mediate relationship between abusive supervision and subordinates’ unethical behavior, and caring climate moderates the positive relationship between turnover intentions and subordinates’ unethical behavior. We also find that the indirect effect is moderated by the caring climate. The theoretical and practical implications are discussed

Numerical simulation of displacement characteristics of CO2 injected in pore-scale porous media

Pore structure of porous media, including pore size and topology, is rather complex. In immiscible two-phase displacement process, the capillary force affected by pore size dominates the two-phase flow in the porous media, affecting displacement results. Direct observation of the flow patterns in the porous media is difficult, and therefore knowledge about the two-phase displacement flow is insufficient. In this paper, a two-dimensional (2D) pore structure was extracted from a sandstone sample, and the flow process that CO2 displaces resident brine in the extracted pore structure was simulated using the Navier–Stokes equation combined with the conservative level set method. The simulation results reveal that the pore throat is a crucial factor for determining CO2 displacement process in the porous media. The two-phase meniscuses in each pore throat were in a self-adjusting process. In the displacement process, CO2 preferentially broke through the maximum pore throat. Before breaking through the maximum pore throat, the pressure of CO2 continually increased, and the curvature and position of two-phase interfaces in the other pore throats adjusted accordingly. Once the maximum pore throat was broken through by the CO2, the capillary force in the other pore throats released accordingly; subsequently, the interfaces withdrew under the effect of capillary fore, preparing for breaking through the next pore throat. Therefore, the two-phase displacement in CO2 injection is accompanied by the breaking through and adjusting of the two-phase interfaces

How does receiving gossip from coworkers influence employees’ task performance and interpersonal deviance? The moderating roles of regulatory focus and the mediating role of vicarious learning

Based on social learning theory and regulatory focus theory, we propose that receiving positive and negative gossip from coworkers increases individuals’ vicarious learning, which subsequently improves their adaptive behaviours in the workplace, namely, increasing task performance and decreasing interpersonal deviance. Furthermore, we propose that gossip receivers’ regulatory foci determine the extent to which receivers engage in vicarious learning and change their behaviours. Results from two studies testing a moderated mediation model (Study 1: N = 214; Study 2: N = 189) showed that negative and positive gossip were indirectly related to increased receivers’ task performance and decreased interpersonal deviance through vicarious learning. The indirect effect of negative gossip was stronger for receivers with a high prevention focus, and the indirect effect of positive gossip was stronger for receivers with a high promotion focus. This research documents the beneficial role of receiving gossip from coworkers and advances our understanding of its consequences in the workplace

The double-edged sword of negative supervisor gossip: When and why negative supervisor gossip promotes versus inhibits feedback seeking behavior among gossip targets

How does being the target of negative supervisor gossip influence the functioning of targeted employees? We draw on feedback intervention theory to examine the beneficial and detrimental effects of negative supervisor gossip on targets’ feedback seeking behavior (FSB). Results from an online scenario study (N = 731) and a multi-wave field study (N = 249) showed that being the target of negative supervisor gossip led to high task reflexivity, which promoted FSB, but also led to high negative affect, which inhibited FSB. Furthermore, targets’ implicit theory of ability moderated the indirect relationships between negative supervisor gossip and FSB. Specifically, negative supervisor gossip stimulated task reflexivity and FSB especially when targets had a strong incremental theory. In contrast, negative supervisor gossip increased negative affect and stifled FSB especially when targets had a strong entity theory. Our findings indicate that negative supervisor gossip is a double-edged sword for targets’ engagement in FSB, thus providing a balanced view of its effects. We provide guidance for supervisors to better deliver and for employees to better receive different forms of feedback

Treatment of chlorpyrifos manufacturing wastewater by peroxide promoted-catalytic wet air oxidation, struvite precipitation, and biological aerated biofilter

Chlorpyrifos manufacturing wastewater (CMW) is characterized by complex composition, high chemical oxygen demand (COD) concentration, and toxicity. An integrated process comprising of peroxide (H2O2) promoted-catalytic wet air oxidation (PP-CWAO), struvite precipitation, and biological aerated filters (BAF) was constructed to treat CMW at a starting COD of 34000–35000 mg/L, total phosphorus (TP) of 5550–5620 mg/L, and total organophosphorus (TOP) of 4700–4840 mg/L. Firstly, PP-CWAO was used to decompose high concentrations of organic components and convert concentrated and recalcitrant TOP to inorganic phosphate. Copper citrate and ferrous citrate were used as the catalysts of PP-CWAO. Under the optimized conditions, 100% TOP was converted to inorganic phosphate with 95.6% COD removal. Then, the PP-CWAO effluent was subjected to struvite precipitation process for recovering phosphorus. At a molar ratio of Mg2+:NH4+:PO43− = 1.1:1.0:1.0, phosphate removal and recovery reached 97.2%. The effluent of struvite precipitation was further treated by the BAF system. Total removals of 99.0%, 95.2%, 97.3%, 100%, and 98.3% were obtained for COD, total suspended solids, TP, TOP, and chroma, respectively. This hybrid process has proved to be an efficient approach for organophosphate pesticide wastewater treatment and phosphorus reclamation

Gasification of coking wastewater in supercritical water adding alkali catalyst

International audienc

Gasification of coking wastewater in supercritical water adding alkali catalyst

International audienc

A novel MBBR–MFC integrated system for high-strength pulp/paper wastewater treatment and bioelectricity generation

International audienceIn this study, a system combining an anaerobic moving-bed biofilm reactor and a microbial fuel cell (MFC) was designed for simultaneous bioelectricity generation and pulp/paper wastewater (PPW) treatment. After 22 days, when hydraulic retention time (HRT) was set at 72 h, ceramsite-added MFC (C-MFC) showed better bioelectricity performance with power density of 94.5 mW/m2 and internal resistance of 35.7 Ω, as compared to the control without ceramsite (W-MFC) (56.1 mW/m2, 54.3 Ω). Chemical oxygen demand (COD) removal efficiencies of C-MFC and W-MFC were 65.6% and 51.3%, respectively. The C-MFC demonstrated its superior electrochemical performance compared to the W-MFC

Impacts of Elevated CO₂ Levels on the Soil Bacterial Community in a Natural CO₂-Enhanced Oil Recovery Area

Knowledge of the interactions among different microorganisms is important to understand how ecological function transformation is affected by elevated CO2 levels in CO2-enhanced oil recovery (CO2-EOR) sites. Molecular ecological networks were established to reveal the interactions among different microbes of the soil bacterial community with the high-throughput sequencing data of 16S rRNA genes. The results showed that these networks are a powerful tool to identify and explain the interactions and keystone species in the communities under elevated CO2 pressure. The structures of networks under different CO2 leakage concentrations were different as a result of the networks’ topology properties, such as node numbers, topological roles of individual nodes, and network hubs. These indicators imply that the interactions among different groups were obviously changed. Moreover, changes in the network structure were significantly correlated with soil pH value, which might suggest that the large CO2 leakage affected the soil ecosystem functions by changing the network interactions. Additionally, the key microbial populations such as Bacteroidetes and Proteobacteria were distinguished based on network topology to reveal community structure and ecosystem functioning. The work developed in this study could help microbiologists to address some research questions that could not be approached previously, and, hence, might represent a new area of research for microbial ecology