Binase Do Not Change Level of Intracellular RNA in Breast Cancer Cells

© 2017, Springer Science+Business Media, LLC. We analyzed changes of intracellular RNA level in breast cancer cells using BT-20, BT-474, and MCF-7 cell lines. It is the continuation of our former studying the effect of bacterial ribonucleases on physiological and biochemical parameters of breast cancer cells. For the first time, we tested ability of Bacillus pumilus RNase (binase) to effect the level of intracellular RNA in these cells. Differences in total RNA level under of the binase action on the cells of triple negative, triple positive, and double positive breast cancer during 48 h of treatment were not detected. The sensitivity of cells to binase was not associated with a reduction of the intracellular RNA level

Features of the development of sports volunteerism in Russia

The promotion of the development and dissemination of volunteerism is one of the priorities of the social and youth policy of the Russian Federation. The purpose of the study is to determine the features of the development of sports volunteerism in Russia, to identify current problems and measures to eliminate them for the sustainable development of sports volunteerism in the subjects of the Russian Federation. Research hypothesis: timely measures to “sports volunteering”, “sports volunteer”, types of sports volunteering activities are specified; the features and stages of development of foreign and Russian sports volunteerism are identified; scientifically based proposals for creating conditions to support the development of modern sports volunteerism in Russia are elaborated. In the course of the study, information, regulatory, statistical and scientific and methodological sources in the field of development of sports volunteerism in Russia are analyzed; the features and problems of this area of volunteer activity and ways to solve them for the effective development of sports volunteerism in the Russian Federation are identified

Social and Cultural Integration and Adaptation of Migrant Children into Norwegian Society in 1990s and 2000s

The authors consider the processes of social and cultural adaptation of migrant children and their integration into the host society in the context of Norway’s immigration policy in the article. Particular attention is paid to the specifics of the country’s immigration policy, strategies for working with immigrant children in the host society, as well as the social consequences of these strategies for Norwegian society. It is emphasized that immigrants and the native population of the country exert mutual influence on each other. Establishment of effective social interactions between them, assimilation of elements of new culture by immigrants has a favorable effect on the internal political stability of the state. It is noted that this is especially important for Norway, which adheres to the policy of multiculturalism. It is concluded that the integration of immigrants into the host community does not happen automatically. The authors also highlight the strengths and weaknesses of the Norwegian immigration policy in relation to children, which to a certain extent calls into question its effectiveness in the long term

Aza-substitution, benzo-annulation effects and catalytic activity of β-octaphenyl-substituted tetrapyrrolic macroheterocyclic cobalt complexes. I. heterogeneous catalysis

© 2016, Springer Science+Business Media Dordrecht.The influence of the size of conjugated π-system on catalytic activity of cobalt complex with β-octaphenylporphyrin and its tetraaza-, tetrabenzo and tetrabenzotetraaza derivatives was studied in present work. It is found that catalytic activity for oxidation of sulfur-containing compounds increases under extension of conjugated macrocycle system according to the following series CoP < CoBP ≤ CoPz < CoPPz ≪ CoPc

THE RESULTS OF STUDYING OF THE MATERIAL FROM THE PATIENTS WITH SUSPECTED SEVERE ACUTE RESPIRATORY SYNDROME IN THE FAR EAST OF RUSSIA

During April-July, 2003 39 specimens of clinical material from six patients and three dead men with suspected «atypical pneumonia» who were detected on the territory of Khabarovsk, Primorski and Sakhalin regions were investigated. The inoculation of clinical material to cell culture Vero E6 did not show the cytopathogenic effect for coronavirus SARS. The amplificate with sought for molecular weight was obtained in RT-PCR assay with primers SAR1 and BNI in sputum and blood specimens of one of the patients, but it was not cDNA of coronavirus SARS. As a result of additional studies there were established the following diagnosis: HFRS, bronchopneumonia with Clebsiella etiology (postmortem) and leptospirosis (postmortem). The terminal diagnosis for the rest patients were: bronchopneumonia of inferior lobes (3), maxillary sinusitis, ARVI and toxocariasis

Countercurrent chromatography in analytical chemistry (IUPAC technical report)

© 2009 IUPACCountercurrent chromatography (CCC) is a generic term covering all forms of liquid-liquid chromatography that use a support-free liquid stationary phase held in place by a simple centrifugal or complex centrifugal force field. Biphasic liquid systems are used with one liquid phase being the stationary phase and the other being the mobile phase. Although initiated almost 30 years ago, CCC lacked reliable columns. This is changing now, and the newly designed centrifuges appearing on the market make excellent CCC columns. This review focuses on the advantages of a liquid stationary phase and addresses the chromatographic theory of CCC. The main difference with classical liquid chromatography (LC) is the variable volume of the stationary phase. There are mainly two different ways to obtain a liquid stationary phase using centrifugal forces, the hydrostatic way and the hydrodynamic way. These two kinds of CCC columns are described and compared. The reported applications of CCC in analytical chemistry and comparison with other separation and enrichment methods show that the technique can be successfully used in the analysis of plants and other natural products, for the separation of biochemicals and pharmaceuticals, for the separation of alkaloids from medical herbs, in food analysis, etc. On the basis of the studies of the last two decades, recommendations are also given for the application of CCC in trace inorganic analysis and in radioanalytical chemistry

АТРОПОИЗОМЕРИЯ 5,10,15,20-ТЕТРАКИС-(3-N-МЕТИЛПИРИДИЛ)-ПОРФИРИНА

The spectral properties of the 5,10,15,20-tetrakis-(3-N-methylpyridyl)-porphyrin were studied in the water solution and the temporal evolution of absorption spectra was revealed. The origin of the spectral changes was found to be due to the solution heterogeneity which is based on the coexistence of atropoisomers. These atropoisomers differ in their pyridyl groups, positioning relative to the mean plane of the tetrapyrrolic macrocycle due to an unrestricted rotation of substituents around the Сm–С1 bond. The observed spectral changes are shown to reflect the redistribution of the atropoisomer concentrations after establishing the equilibrium distribution that is over approximately within 300 hours after the preparation of a solution at a temperature of 290 ± 2 K. The atropoisomerization is suggested to be the inherent property of all 5,10,15,20-tetraarylporphyrins with an asymmetric substitution of aryl groups with respect to the axis passing through the Сm–С1 bond. The prospects for the practical applications of the revealed phenomenon are discussed. Исследованы спектральные характеристики 5,10,15,20-тетра-(3-N-метилпиридил)-порфирина в во- дном растворе и обнаружена временная эволюция спектров поглощения. Установлено, что причиной спектральной эволюции является гетерогенность раствора, обусловленная наличием нескольких атропоизомеров, различающихся положением пиридильных групп относительно средней плоскости тетрапиррольного макроцикла молекулы порфирина благодаря свободному вращению заместителей вокруг связи Сm–С1. Показано, что наблюдаемые спектральные превращения отражают перераспределение концентраций атропоизомеров в ходе установления равновесного распределения, которое завершается примерно через 300 ч после приготовления раствора при температуре 290 ± 2 К. Предложено, что явление атропоизомерии следует считать присущим всем 5,10,15,20-тетраарилпорфиринам с асимметричным относительно проходящей через связь Сm–С1 оси замещением арильных групп. Обсуждены возможности практического использования данного явления.

Results of Monitoring over and Biological Properties of <I>Vibrio cholerae</I> Isolated from Ambient Environment Objects in the Khabarovsk Territory

genotype and a unique MLVA profile. The results of the monitoring indicated that there were optimal for V. cholerae accumulation in surface water reservoirs conditions and it was necessary to enhance measures for cholera prophylaxis in the post-flood period

Analysis of Epidemiological Situation on Hemorrhagic Fever with Renal Syndrome in the Khabarovsk Territory and the Jewish Autonomous Region, and Prognosis of Its Development for the Past-Flood Period in 2013-2014

Complex analysis of the data concerning epidemiological situation on HFRS in the Khabarovsk Territory and the Jewish Autonomous Region for the past decade (2003-2012) previous to high water in 2013 and results of operative epidemiological inspection of the flooding area and the adjoining territory where a high index of laboratory evidences of the virus presence in the main carriers was revealed, outlines high probability of the epidemiological condition complications in view of the infection in the post-flooding period in 2013-2014 in the both entities. Therewith on the basis of the unfavorable prognosis for the epidemiological situation development a Plan of organizational, preventive and anti-epidemic measures directed to minimization of risks related to aggravation of epidemiological situation on hemorrhagic fever with renal syndrome has been developed and is being implemented

Epizootiological-and-Epidemical Situation on Tularemia in the South of Far East Region during the Period of River Floods

Carried out were epizootiological studies of the flooded areas of the Amur Region, the Khabarovsk territory, and the Jewish autonomous region in order to investigate the situation on tularemia in view of the river floods in August-September, 2013. Involved were specialized anti-epidemic teams from Irkutsk Research Anti-Plague Institute for works at the site of emergency. Therewith, analysis of the data of the epizootiological examination of the territories obtained in cooperation with specialists from the Rospotrebnadzor local Centers of Hygiene and Epidemiology, and results of laboratory investigations with application of bacteriological, immu­no­bio­logical and molecular-genetic methods indicated insignificant epizootic and epidemic activity of the natural tularemia foci. Given is the conclusion on epizootiological-epidemiological condition for tularemia in the flooded areas of the Amur River region and a short-term epizootiological forecast for 2013-2014, as well as recommendations concerning stabilization and improvement of epidemiological conditions in the region