Let us conserve and exchange seeds: celebrating traditional crop diversity of the Nepali lowlands

A seed fair is an activity to create awareness about and appreciate local crop diversity, exchange seed and related knowledge, and celebrate farmers’ efforts to conserve agrobiodiversity. It takes considerable time and effort to organize a seed fair. This brief describes the seed fair organized at the Agyauli Community Seedbank, Nawalparasi in the southern region of Nepal. About 30 members of 10 community seedbanks from the terai (the southern lowland) region of Nepal came together for this. Apart from exchanging seeds of traditional crop varieties, they also shared stories about the socio-cultural, religious, spiritual, nutritional and medicinal values of their varieties. The recent formal registration of the Community Seed Banks Association of Nepal (CSBAN) was also celebrated

Adaptability of Naked Barley Landraces in Mountain Agro-ecosystem of Nepal

Naked barley (Hordeum vulgare var. nudum L.) is an important food crop in mountain regions of Nepal, however, its production area and genetic diversity is known to be shrinking fast. One of the reasons could be the poor productivity. To improve the productivity of crops, it is essential that new site-specific, high-yielding and widely adopted varietal options for farmers should be developed for sustainability. To identify the varietal responses to environment, multi-location testing is one of the main tools. Following this, we evaluated agronomic performance of eight naked barley landraces collected from five different locations of Nepal with altitude ranging from 1370 to 2500 meter elevations from the sea level during the winter season of 2014/15 in order to assess the landraces adaptability across different locations. Combined analysis of variances revealed that NGRC02306, NGRC04902 and NGRC04894 were the high yielding landraces. The landrace namely NGRC04894 was found the most stable genotype with better adaptability to all tested environments whereas NGRC02306 and NGRC04902 were high yielding landraces adapted to high yielding environment namely Khumaltar. However, the lower yielding landrace NGRC02327 was the earliest and could be preferred by farmers as its maturity allows it to fit better in the rice based cropping system. Our study showed that these landraces need to be verified further in farmers' fields and the release of one or more of them would help to diversify the genetic base of naked barley varieties in the seed supply system

Bacteriological profile and antibiotic sensitivity pattern in patients with Urinary tract infection

Background: Urinary tract infection is one of the common bacterial infections seeking treatment in clinical practice. A variety of organisms are associated with UTI and the most common organisms are Escherichia coli and other coliforms. Bacteriological investigations of UTI are not complete without antibiotic sensitivity test of the isolate. The aim of this study is to determine the bacteriological profile and antibiotic sensitivity patterns and their disease association.Materials and methods: This study was conducted in Shankarapur Hospital over a period of one year. All cases of suspected UTI sent for urine culture and sensitivity test were evaluated in this study. Disease associated with UTI, bacteriological profile and antibiotic sensitivity patterns were evaluated.Results: A total of 974 cases were sent for urine culture and sensitivity test. The total culture positive cases were 165 (17.4%).  The most common age group for culture positive test was 21- 30 years (33.3%) followed by 31- 40 years (25.5%). Female patients formed the majority of culture positive cases (84.8%) and E Coli (86.1%) was the most common organism isolated. Piperacillin- tazobactum and ceftriaxone were the most common antibiotic sensitive to the organisms. Simple uncomplicated UTI and PID were the most common indication for subjecting the patients to urine culture.Conclusion: UTI is most commonly seen in female of reproductive age group and the most common causative organism is E coli. Culture result and antibiogram helps the clinician for specific treatment of UTI.</p

Nodule crushing: a novel technique to decentralize rhizobia inoculant technology and empower small-scale farmers to enhance legume production and income

Millions of small scale legume farmers lack access to rhizobia bacterial inoculants that improve crop protein and yield, and minimize fossil-fuel based nitrogen fertilizers, through biological nitrogen fixation (BNF). BNF converts atmospheric nitrogen gas into ammonia, required to synthesize chlorophyll and amino acids. BNF is catalyzed by rhizobia that inhabit nodule organs in legume roots (e.g., soybean, cowpea, chickpeas, lentil, fava, peas, beans). Rhizobia inoculant access in remote communities is limited by centralized facilities to grow bacteria, inadequate transportation networks and refrigeration. Recently, we proposed that rhizobia inoculants can be diffused by farmers themselves, simply, by crushing nodules onto seeds, and demonstrated its efficacy under field conditions. A concern was whether nodules remain viable between growing seasons. Here we provide preliminary evidence that bean nodules, dried and stored at room temperature after 6 months, retain nodulation potential. We discuss: (1) the feasibility, constraints and risks of nodule crushing; (2) scaling up strategies; (3) entrepreneurship that benefits women farmers (e.g., nodule-selling microenterprises); and (4) empowering farmers to directly select, evolve and indigenize rhizobia for the first time. Finally, we prioritize research questions and encourage the formation of a global participatory research network, with the goal of decentralizing and democratizing rhizobia inoculants

Effect of Layer Thickness on Structural, Morphological and Superconducting Properties of Nb\u3csub\u3e3\u3c/sub\u3eSn Films Fabricated by Multilayer Sequential Sputtering

Superconducting Nb3Sn films can be synthesized by controlling the atomic concentration of Sn. Multilayer sequential sputtering of Nb and Sn thin films followed by high temperature annealing is considered as a method to fabricate Nb3Sn films, where the Sn composition of the deposited films can be controlled by the thickness of alternating Nb and Sn layers. We report on the structural, morphological and superconducting properties of Nb3Sn films fabricated by multilayer sequential sputtering of Nb and Sn films on sapphire substrates followed by annealing at 950 °C for 3 h. We have investigated the effect of Nb and Sn layer thickness and Nb:Sn ratio on the properties of the Nb3Sn films. The crystal structure, surface morphology, surface topography, and film composition were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), atomic force microscopy (AFM), and energy dispersive X-ray spectroscopy (EDS). The results showed Sn loss from the surface due to evaporation during annealing. Superconducting Nb3Sn films of critical temperature up to 17.93 K were fabricated

Magnetron Sputtering of Nb\u3csub\u3e3\u3c/sub\u3eSn for SRF Cavities

Nb3Sn is a potential candidate for surface material of SRF cavities since it can enable the cavity to operate at higher temperatures with high quality factor and at an increased accelerating gradient. Nb-Sn films were deposited using magnetron sputtering of individual Nb and Sn targets onto Nb and sapphire substrates. The as-deposited films were annealed at 1200 °C for 3 hours. The films were characterized for their structure by X-ray Diffraction (XRD), morphology by Field Emission Scanning Electron Microscopy (FESEM), and composition by Energy Dispersive X-ray Spectroscopy (EDS) and Time of Flight Secondary Ion Mass Spectrometry (ToF-SIMS). The surface resistivity was measured down to cryogenic temperature to determine the superconducting transition temperature and its width. The composition of the multilayered films was controlled by varying the thickness of the Nb and Sn layers. The films showed crystalline Nb3Sn phases with Tc up to 17.6 K

Deposition of Nb₃Sn Films by Multilayer Sequential Sputtering for SRF Cavity Application

Nb3Sn is considered as an alternative of Nb for SRF accelerator cavity application due to its potential to obtain higher quality factors and higher accelerating gradients at a higher operating temperature. Magnetron sputtering is one of the effective techniques that can be used to fabricate Nb3Sn on SRF cavity surface. We report on the surface properties of Nb3Sn films fabricated by sputtering multiple layers of Nb and Sn on sapphire and niobium substrates followed by annealing at 950°C for 3 h. The crystal structure, film microstructure, composition and surface roughness were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and atomic force microscopy (AFM). The RF performance of the Nb3Sn coated Nb substrates were measured by a surface impedance characterization system. We also report on the design of a multilayer sputter deposition system to coat a single-cell SRF cavity

A novel approach for implementing community seed banks in the mountain area of Nepal

Community seed bank (CSB) is one of the approaches successfully applied in Nepal and globally for conservation and use of local crop diversity for food and agriculture. However, there are only a few success examples of CSBs in high mountain areas of Nepal, particularly focusing on traditional underutilized crops. This chapter aims to present the modality and experiences of implementing community seed banks in mountain districts, namely, Humla, Jumla, Lamjung and Dolakha, by the UNEP GEF Local Crop Project (LCP) jointly implemented by Bioversity International, NAGRC, DoA and LI-BIRD. The project was implemented in 2014 in Nepal for providing diversity rich solutions and mainstreaming the conservation and use of local agricultural biodiversity in the mountain agricultural production landscapes. The LCP adopted Diversity Field School (DFS) approach to sensitize and mobilize communities about the importance of local crop genetic resources, paving way in realizing the need of CSB and its institutionalization. DFS approach has been found effective to identify and mobilize custodians of agrobiodiversity, promote good practices such as participatory variety selection, grass-roots breeding, food fairs and promote farmer- to-farmer learning and sharing as a part of local capacity building processes. DFS approach has empowered women farmers to take leadership roles in managing community seed banks. The project engaged local governments (Palikas) from the beginning to make them realize the vital need of CSBs, strengthen local seed systems as a mechanism to increase access to quality seeds of traditional and underutilized crop species. It has resulted a positive outcome to develop ownership on institutionalizing CSB in local agriculture development plans and providing significant amount of financial resources and material support to establish and sustain CSBs in LCP sites. In remote high mountain areas, where seed business opportunity is limited; engagement of and ownership of local government is crucial to sustain CSBs

Symbiotic nitrogen fixation, growth and yield response of common bean and cowpea for Rhizobia inoculation and micronutrients (B and Mo) application in mid-hill regions of Nepal

This is a CIFSIRF funded project.Challenges associated with legume production in Nepal include: lack of high yielding varieties and crop specific management (e.g., disease/pest management) practices, loss of soil fertility including micronutrients, poor access to agricultural inputs and services, shortage of irrigation water, lack of mechanization, and labour shortages which led to poor yields and low economic returns to farmers (Chapagain and Gurung, 2010; Chapagain and Raizada, 2017). Farmers mostly used untreated seeds from previous season harvest to cultivate these crops and very little attention has been given to study the effects of bacterial inoculation on the crop performance and soil health through systemic on-farm trials. Symbiotic nitrogen fixation of 3 legumes can be improved by introducing efficient rhizobia strains for N fixation (Thilakarathna and Raizada, 2017), and also through application of soil deficient micronutrients which are important for different stages of SNF (Weisany et al., 2013). This research explores the opportunities to use different rhizobia strains (native as well as exotic) along with micronutrients (Boron and Molybdenum) on two legume crops (e.g., cowpea and common bean) to enhance SNF, crop yields (grain and plant biomass), plant and grain N content, and offers the most productive combination(s) for each crop