Upper Agri Valley (Basilicata) between Geomorphology and Ancient Settlements

In this paper we present the geoarchaeological and landscape project on the Upper Agri Valley (Basilicata). Between 2012 and 2015 we coordinated multidisciplinary research with the purpose of reconstructing the history and evolution of the territory, analysing the relationships between man and landscape from the Prehistory to the Middle Age. The studied area, an inland mountain valley situated in the ancient Lucania, is very important, because it is a fundamental route between the two coasts of Magna Graecia and because during the Roman period this valley was the territory in which Grumentum was born, one of the most important towns of Roman Southern Italy. Thanks to the dialogue between different competences, this project aims at understanding the settlements dynamics and the mutual influences between man and environment: indeed, we conducted geophysics and geoarchaeological investigations, archaeological surveys, cartographic and aerial photos studies, written sources analysis: in this way, different data are used to understand the landscape from a global point of view

Modulation of hepatic lipid metabolism by olive oil and its phenols in nonalcoholic fatty liver disease

Nonalcoholic fatty liver disease (NAFLD) represents the most common chronic liver disease in western countries, being considered the hepatic manifestation of metabolic syndrome. Cumulative lines of evidence suggest that olive oil, used as primary source of fat by Mediterranean populations, may play a key role in the observed health benefits on NAFLD. In this review, we summarize the state of the art of the knowledge on the protective role of both major and minor components of olive oil on lipid metabolism during NAFLD. In particular, the biochemical mechanisms responsible for the increase or decrease in hepatic lipid content are critically analyzed, taking into account that several studies have often provided different and/or conflicting results in animal models fed on olive oil-enriched diet. In addition, new findings that highlight the hypolipidemic and the antisteatotic actions of olive oil phenols are presented. As mitochondrial dysfunction plays a key role in the pathogenesis of NAFLD, the targeting of these organelles with olive oil phenols as a powerful therapeutic approach is also discussed

Oleic Acid and Hydroxytyrosol Inhibit Cholesterol and Fatty Acid Synthesis in C6 Glioma Cells

Recently, the discovery of natural compounds capable of modulating nervous system function has revealed new perspectives for a healthier brain. Here, we investigated the effects of oleic acid (OA) and hydroxytyrosol (HTyr), two important extra virgin olive oil compounds, on lipid synthesis in C6 glioma cells. OA and HTyr inhibited both de novo fatty acid and cholesterol syntheses without affecting cell viability. The inhibitory effect of the individual compounds was more pronounced if OA and HTyr were administered in combination. A reduction of polar lipid biosynthesis was also detected, while triglyceride synthesis was marginally affected. To clarify the lipid-lowering mechanism of these compounds, their effects on the activity of key enzymes of fatty acid biosynthesis (acetyl-CoA carboxylase-ACC and fatty acid synthase-FAS) and cholesterologenesis (3-hydroxy-3-methylglutaryl-CoA reductase-HMGCR) were investigated in situ by using digitonin-permeabilized C6 cells. ACC and HMGCR activities were especially reduced after 4 h of 25 μM OA and HTyr treatment. No change in FAS activity was observed. Inhibition of ACC and HMGCR activities is corroborated by the decrease of their mRNA abundance and protein level. Our results indicate a direct and rapid downregulatory effect of the two olive oil compounds on lipid synthesis in C6 cells

ERα-LBD, an isoform of estrogen receptor alpha, promotes breast cancer proliferation and endocrine resistance

Estrogen receptor alpha (ER alpha) drives mammary gland development and breast cancer (BC) growth through an evolutionarily conserved linkage of DNA binding and hormone activation functions. Therapeutic targeting of the hormone binding pocket is a widely utilized and successful strategy for breast cancer prevention and treatment. However, resistance to this endocrine therapy is frequently encountered and may occur through bypass or reactivation of ER-regulated transcriptional programs. We now identify the induction of an ER alpha isoform, ER alpha-LBD, that is encoded by an alternative ESR1 transcript and lacks the activation function and DNA binding domains. Despite lacking the transcriptional activity, ER alpha-LBD is found to promote breast cancer growth and resistance to the ER alpha antagonist fulvestrant. ER alpha-LBD is predominantly localized to the cytoplasm and mitochondria of BC cells and leads to enhanced glycolysis, respiration and stem-like features. Intriguingly, ER alpha-LBD expression and function does not appear to be restricted to cancers that express full length ER alpha but also promotes growth of triple-negative breast cancers and ER alpha-LBD transcript (ESR1-LBD) is also present in BC samples from both ER alpha(+) and ER alpha(-) human tumors. These findings point to ER alpha-LBD as a potential mediator of breast cancer progression and therapy resistance

A História da Alimentação: balizas historiográficas

Os M. pretenderam traçar um quadro da História da Alimentação, não como um novo ramo epistemológico da disciplina, mas como um campo em desenvolvimento de práticas e atividades especializadas, incluindo pesquisa, formação, publicações, associações, encontros acadêmicos, etc. Um breve relato das condições em que tal campo se assentou faz-se preceder de um panorama dos estudos de alimentação e temas correia tos, em geral, segundo cinco abardagens Ia biológica, a econômica, a social, a cultural e a filosófica!, assim como da identificação das contribuições mais relevantes da Antropologia, Arqueologia, Sociologia e Geografia. A fim de comentar a multiforme e volumosa bibliografia histórica, foi ela organizada segundo critérios morfológicos. A seguir, alguns tópicos importantes mereceram tratamento à parte: a fome, o alimento e o domínio religioso, as descobertas européias e a difusão mundial de alimentos, gosto e gastronomia. O artigo se encerra com um rápido balanço crítico da historiografia brasileira sobre o tema

Assessment of alterations in the structure and function of the enteric nervous system in a murine model of multiple sclerosis

reservedLa sclerosi multipla (SM) è una malattia neurodegenerativa e demielinizzante di origine autoimmune che colpisce il sistema nervoso centrale (SNC). Tuttavia numerose evidenze scientifiche recenti suggeriscono una connessione bidirezionali tra il SNC e il sistema nervoso enterico (SNE) coinvolte nella patogenesi di questa malattia. Questo studio, quindi, mira a valutare l’impatto della demielinizzazione indotta dalla neurotossina cuprizone sulla struttura e funzione del SNE in un modello murino di SM. Sono state condotte analisi in vivo ed ex vivo della funzionalità contrattile del piccolo intestino e analisi in vitro attraverso microscopia confocale per valutare la presenza di alterazioni strutturali nella rete neurogliale del SNE, causate dalla demielinizzazione tossica. Infine attraverso analisi quantitativa con RT-qPCR è stata valutata l’espressione di un pool di geni in specifiche aree celebrali ed ileo. Questa ricerca fornisce importanti spunti per comprendere meglio il legame tra la SM e i disturbi gastrointestinali, aprendo nuove prospettive per ulteriori studi e l'identificazione di potenziali bersagli terapeutici per migliorare la qualità di vita dei pazienti con SM e i relativi sintomi intestinali.Multiple sclerosis (MS) is an autoimmune neurodegenerative and demyelinating disease that affects the central nervous system (CNS). However, numerous recent scientific findings suggest a bidirectional connection between the CNS and the enteric nervous system (ENS) involved in the pathogenesis of this disease. Therefore, this study aims to assess the impact of cuprizone-induced demyelination on the structure and function of the ENS in a murine model of MS. In vivo and ex vivo analyses of small intestine contractile functionality were conducted, and in vitro analyses were performed through confocal microscopy to evaluate the presence of structural alterations in the neuroglial network of the ENS caused by toxic demyelination. Finally, quantitative analysis using RT-qPCR assessed the expression of a gene pool in specific brain areas and the ileum. This research provides important insights into better understanding the link between MS and gastrointestinal disorders, opening new perspectives for further studies and the identification of potential therapeutic targets to improve the quality of life of MS patients and their associated intestinal symptoms