T-Cell Subsets Predict Mortality in Malnourished Zambian Adults Initiating Antiretroviral Therapy.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedTo estimate the prognostic value of T-cell subsets in Zambian patients initiating antiretroviral therapy (ART), and to assess the impact of a nutritional intervention on T-cell subsets.This work was supported by European and Developing Countries Clinical Trials Partnership grant # IP.2009.33011.004; trial foods were prepared and supplied by Nutriset, Malauney, Franc

Modified Single Prolonged Stress Reduces Cocaine Self-Administration During Acquisition Regardless of Rearing Environment

Until recently, there were few rodent models available to study the interaction of post-traumatic stress disorder (PTSD) and drug taking. Like PTSD, single prolonged stress (SPS) produces hypothalamic-pituitary-adrenal (HPA) axis dysfunction and alters psychostimulant self-administration. Other stressors, such as isolation stress, also alter psychostimulant self-administration. However, it is currently unknown if isolation housing combined with SPS can alter the acquisition or maintenance of cocaine self-administration. The current study applied modified SPS (modSPS; two hours restraint immediately followed by cold swim stress) to rats raised in an isolation condition (Iso), enrichment condition (Enr), or standard condition (Std) to measure changes in cocaine self-administration and HPA markers. Regardless of rearing condition, rats exposed to modSPS had greater corticosterone (CORT) release and reduced cocaine self-administration during initial acquisition compared to non-stressed controls. In addition, during initial acquisition, rats that received both Iso rearing and modSPS showed a more rapid increase in cocaine self-administration across sessions compared to Enr and Std rats exposed to modSPS. Following initial acquisition, a dose response analysis showed that Iso rats were overall most sensitive to changes in cocaine unit dose; however, modSPS had no effect on the cocaine dose response curve. Further, there was no effect of either modSPS or differential rearing on expression of glucocorticoid receptor (GR) in hypothalamus, medial prefrontal cortex, amygdala, or nucleus accumbens. By using modSPS in combination with Iso housing, this study identified unique contributions of each stressor to acquisition of cocaine self-administration

Mollusk carbonate thermal behaviour and its implications in understanding prehistoric fire events in shell middens

Archaeological shell middens are particularly important for reconstructing prehistoric human subsistence strategies. However, very little is known about shellfish processing, especially when related to the use of fire for dietary and disposal purposes. To shed light on prehistoric food processing techniques, an experimental study was undertaken on modern gastropod shells (Phorcus lineatus). The shells were exposed to high temperatures (200-700 °C) to investigate subsequent mineralogy and macro- and microstructural changes. Afterwards, the three-pronged approach was applied to archaeological shells from Haua Fteah cave, Libya (Phorcus turbinatus) and from shell midden sites in the United Arab Emirates (Anadara uropigimelana and Terebralia palustris) to determine exposure temperatures. Results indicated that shells from the Haua Fteah were exposed to high temperatures (600 - 700 °C) during the Mesolithic period (c. 12.7 - 9 ka), whereas specimens from the Neolithic period (c. 8.5 - 5.4 ka) were mainly exposed to lower temperatures (300 - 500 °C). The thermally-induced changes in A. uropigimelana and T. palustris shells from the South East Arabian archaeological sites were similar to those seen in Phorcus spp. suggesting a broad applicability of the experimental results at an interspecific level. Although heat significantly altered the appearance and mineralogy of the shells, 14CAMS ages obtained on burnt shells fit within the expected age ranges for their associated archaeological contexts, indicating that robust radiocarbon ages may still be obtained from burnt shells. Our study indicates that the combination of microstructural and mineralogical observations can provide important information to infer shellfish processing strategies in prehistoric cultures and their change through time.Funding for this study was kindly provided by the EU within the framework (FP7) of the Marie Curie International Training Network ARAMACC (604802) to SM and by the Alexander von Humboldt Postdoctoral Fellowship (1151310) and McKenzie Postdoctoral Fellowship to AP. The Haua Fteah excavations were undertaken with the permission of the Libyan Department of Antiquities and with funding to GB from the Society for Libyan Studies and from the European Research Council (Advanced Investigator Grant 230421), whose support is also gratefully acknowledge