2 research outputs found
Detection and analysis of genetic variations in species of <i style="mso-bidi-font-style: normal">Plumbago </i>L. using RAPD markers
140-143In the present study, random amplified
polymorphic DNA (RAPD) markers were used to measure genetic diversity of 3
species of Plumbago, viz., <i style="mso-bidi-font-style:
normal">P. zeylanica L., P. rosea
L. and P. <i style="mso-bidi-font-style:
normal">auriculata Lam., grown in India. Thirty nine decamer
oligonucleotide primers were used to amplify the genomic DNA isolated from leaf
samples. Of the primers used, only 19 primers gave clear reproducible bands in
various ranges. A total of 299 amplified bands were scored, of which 107 were
found to be polymorphic bands (PPB), i.e.,
35.79%. The data obtained from the amplified bands were used in NTSYS PC
Version 2.1 software and a cladogram was constructed showing the genetic
distance between species. The results reveal that <i style="mso-bidi-font-style:
normal">P. rosea and P. zeylanica
were present in the same cluster, whereas
P. auriculata showed divergence. Nei’s and Li’s similarity coefficients and
GSI were also calculated