Photodynamic therapy of oral leukoplakia and oral lichen planus using methylene blue: A pilot study

Premalignant lesions like oral lichen planus (OLP), oral leukoplakia (OL) has a fair probability of transforming into malignancy and they are perverse toward conventional therapies. Photodynamic therapy (PDT) has been considered as an alternative/complimentary therapeutic modality for the management of premalignant lesions. In this study, methylene blue-mediated photodynamic therapy (MB-PDT) was used as a possible alternative method for the treatment of OLP and OL. A total of 15 OLP lesions and 13 OL lesions were enrolled in the study. The patients were irradiated using metal halide lamp filtered at 630 ± 10 nm, with a light exposure dose of 120 J/cm2 per sitting. For the OLP lesions, MB-PDT was performed once a week for four weeks and for the OL lesions, MB-PDT was performed twice a week for three weeks. Lesions were evaluated pre- and post- and at follow-up sessions by changes in sign and symptom scores, and size of lesions. We have observed a 53.3% of complete reduction in the treated OLP lesions and their decrease in size, sign and symptom score after treatment and at follow-up session was statistically significant. We have also observed complete response for one OL lesion of the 13 treated lesions. The result indicates that MB-PDT is an effective modality in management of OLP and OL. Among the two types of premalignancies treated with MB-PDT, OLP lesions responded much better than that of OL

Investigation of Phloroglucinol Succinic Acid Dendrimer as Antimicrobial Agent Against Staphylococcus Aureus, Escherichia Coli and Candida Albicans

In this study, antimicrobial investigations for the efficiently synthesized biocompatible Phloroglucinol Succinic acid (PGSA) dendrimer with anionic surfaces were performed using broth dilution method against a Gram-positive bacterium (Staphylococcus aureus), a Gram-negative bacterium (Escherichia coli) and a fungal human pathogen (Candida albicans) to determine the minimal inhibitory concentration (MIC) value. Additionally, fluorescence and UV absorbance spectroscopy techniques were used to monitor the release of intracellular materials from the pathogens owing to anionic dendrimers. The exact binding sites of this dendrimer on these pathogens by molecular modelling studies motivated us to report this nanocarrier as a new antimicrobial agent