西ナイルウイルス不活化ワクチンの開発と評価

長崎大学学位論文 [学位記番号]博(医歯薬)甲第397号 [学位授与年月日]平成23年2月23

Research funding: What do I need before I start looking for a funding source? Part 1

Esta es la primera parte de un artículo sobre la búsqueda de financiamiento para un proyecto de investigación. Esta entrega resume los principales ítems para tener en consideración a la hora de postularse a una convocatoria. Requerimientos del proceso: 1. Tiempo protegido. 2. Propuesta de investigación sólida. 3. Equipo calificado y con experiencia. 4. Definición y organización de actividades. 5. Cronograma de actividades. 6. Estimación de costosThis is the first part of an article about finding funding for a research project. This delivery summarizes the main ítems to take into consideration when applying for a call. Process requirements: 1. Protected time. 2. Strong research proposal. 3. Qualified and experienced team. 4. Definition and organization of activities. 5. Schedule of activities. 6. Cost estimateFil: Posadas Martinez, Maria Lourdes. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Grande Ratti, Maria Florencia. Hospital Italiano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Burgos, Mariana. Hospital Italiano; ArgentinaFil: Herrera, Víctor. Hospital Italiano; Argentin

Ameliorative Effect of the Oral Administration of Chuquiraga spinosa in a Murine Model of Breast Cancer Induced with 7,12-Dimethylbenz[a]anthracene (DMBA)

Objective: To determine the ameliorative effect of the ethanolic extract of Chuquiraga spinosa (ChS) on 7,12-Dimethylbenz[a]anthracene (DMBA)-induced breast cancer in rats. Methods: 36 female Holztman rats were divided into 6 groups. I) The negative control group received physiological saline (PS). II) ChS-200 group received 200 mg/kg of ChS. III) DMBA group was induced with DMBA (20 mg/Kg) dissolved in PS and administrated orally for 15 weeks. IV) DMBA + ChS-50 group, V) DMBA + ChS-250 group, and VI) DMBA + ChS-500 group, which received the extract orally for 15 weeks after DMBA induction. All data were expressed as mean and standard deviation. One-way analysis of variance (ANOVA) followed by Dunnet test was carried out to compare the mean value of different groups Histopathological analysis was evaluated by using Image J software. Results: Hematology showed that the triglyceride level was significantly lowered (P< 0.01) and high-density lipoprotein (HDL) level was significantly increased (P <0.01) in groups III, IV and V. Also, ChS extract significantly lowered the C reactive protein (CRP) level (P <0.01) and malondialdehyde level (P<0.05). There was a significant decrease in the frequency of DMBA-induced micronucleated polychromatic erythrocyte (P<0.01). Conclusions: Chuquiraga spinosa showed an ameliorative effect on DMBA-induced breast cancer in rats as well as antioxidant, antitumor and antigenotoxic properties.Revisión por pare

Competencias del Docente en Enfermería en la Elaboración de la Planeación Didáctica

Objetivo: Describir el nivel de competencias profesionales para elaborar una planeación didáctica en los docentes de enfermería de una universidad pública del estado de Puebla y Tabasco. Métodos: El diseño del estudio fue cuantitativo, transversal, descriptivo comparativo de dos Instituciones de Educación Superior. El muestreo fue por conveniencia, con una muestra final de 20 docentes de enfermería de pregrado; 10 del estado de Puebla y 10 de Tabasco. Se utilizó una cédula ex profesa de datos personales y se aplicó un instrumento para evaluar las competencias del docente de enfermería en la planeación didáctica, con un total de 12 reactivos.  Este instrumento tiene dos dimensiones; conocimientos y habilidades prácticas del docente. Resultados principales: Se muestra que el 40% de los docentes de enfermería tiene un nivel de competencia bajo y el 30% se encuentra entre un nivel moderado y alto con respecto al conocimiento de la planeación didáctica. Asimismo, referente a las habilidades prácticas, el 65% se ubica en nivel de competencia bajo y tan sólo el 15% presenta un nivel de competencia alto. Conclusión general: La planeación didáctica en enfermería es esencial para asegurar que los estudiantes reciban una educación de calidad y estén preparados para enfrentar los desafíos del campo de la enfermería

Ameliorative Effect of the Oral Administration of Chuquiraga spinosa in a Murine Model of Breast Cancer Induced with 7,12-Dimethylbenz[a]anthracene (DMBA)

Objective: To determine the ameliorative effect of the ethanolic extract of Chuquiraga spinosa (ChS) on 7,12-Dimethylbenz[a]anthracene (DMBA)-induced breast cancer in rats. Methods: 36 female Holztman rats were divided into 6 groups. I) The negative control group received physiological saline (PS). II) ChS-200 group received 200 mg/kg of ChS. III) DMBA group was induced with DMBA (20 mg/Kg) dissolved in PS and administrated orally for 15 weeks. IV) DMBA + ChS-50 group, V) DMBA + ChS-250 group, and VI) DMBA + ChS-500 group, which received the extract orally for 15 weeks after DMBA induction. All data were expressed as mean and standard deviation. One-way analysis of variance (ANOVA) followed by Dunnet test was carried out to compare the mean value of different groups Histopathological analysis was evaluated by using Image J software. Results: Hematology showed that the triglyceride level was significantly lowered (P< 0.01) and high-density lipoprotein (HDL) level was significantly increased (P <0.01) in groups III, IV and V. Also, ChS extract significantly lowered the C reactive protein (CRP) level (P <0.01) and malondialdehyde level (P<0.05). There was a significant decrease in the frequency of DMBA-induced micronucleated polychromatic erythrocyte (P<0.01). Conclusions: Chuquiraga spinosa showed an ameliorative effect on DMBA-induced breast cancer in rats as well as antioxidant, antitumor and antigenotoxic properties

Morphological identification and characterization of the formation of floral primordium in Vanilla planifolia (Orchidaceae)

ABSTRACT Objective: To study the formation of floral primordia and individual flower development in the inflorescence of vanilla (Vanilla planifolia Jacks Ex. Andrews). Design/methodology/approach: The study site was located at Rancho Xanathtlan, in Barriles, municipality of Gutiérrez Zamora, Veracruz. Inflorescence primordia and young inflorescences (racemes) at different stages of development were sampled from three different stem positions: basal (11-15 internodes), middle (6-10 internodes) and distal (1-5 internodes). Four samplings were made from February to May, and five replicates of each stem position were used per sampling date. Observations and characterization were carried out with a stereo microscope. Results: Seven developmental stages were described for the racemes of V. planifolia: (I) differentiated meristem of the floral inflorescence, (II) appearance of the third bract, (III) initiation of the formation of the racemes, (IV) elongation of the floral primordia, (V) development and growth of the individual floral primordia in the acropetal direction, (VI) anthesis of the inflorescence in the acropetal direction and (VII) complete flowering of the raceme. Five developmental stages of formation of individual flower primordia within the inflorescence were also identified, which occurred from the appearance of the third bract in the acropetal direction. Limitations on study/implications: Although this study denotes the first observations on the floral phenology of V. planifolia, there are still many questions about the biochemical processes and the interaction of environmental aspects in this floral development. Findings/conclusions: First aspects of floral formation within the inflorescence as well as floral phenology in Vanilla planifolia were shown. Keywords: Floral growth, floral phenology, inflorescences, orchids.Objective: To morphologically identify and characterize the formation of floral primordium and the individual flower development in Vanilla planifolia Jacks Ex. Andrews. Design/Methodology/Approach: Inflorescence primordia and young inflorescences in different development stages were sampled from the stem internodes of the following positions: basal (11-15), middle (6-10), and distal (1-5). Four samples were taken from each stem position from February to May, with five repetitions per sampling date. Observations and characterization were made with a stereo microscope. The study site was located at Rancho Xanathtlan, in Barriles, municipality of Gutiérrez Zamora, Veracruz. Results: The development of the V. planifolia raceme is described in seven phases: (I) differentiated meristem of the floral inflorescence; (II) appearance of the third bract; (III) initiation of the racemes formation; (IV) elongation of the floral primordium; (V) development and growth of the individual floral primordium in the acropetal direction, (VI) anthesis of the inflorescence in the acropetal direction; and (VII) complete flowering of the raceme. The development of the buds in the raceme is described in five stages from the appearance of the third bract in the acropetal direction. Study Limitations/Implications: The biochemical processes and the interaction of environmental aspects on the floral development of V. planifolia pose questions that remain unanswered. Findings/Conclusions: The first aspects of floral formation within the inflorescence of Vanilla planifolia were determined, along with its floral phenology

Estudio comparativo de la capacidad aeróbica y respuesta cardiovascular en estudiantes universitarios de méxico y colombia

Introducción y objetivo:El test de20 Meter Shuttle Run Test(20mSRT), es una prueba de aptitud cardiorrespiratoria que mide potencia aeróbica máxima e indirectamente el consumo máximo de oxígeno; El objetivo fue comparar la capacidad aeróbica y respuesta cardiopulmonar de jóvenes universitarios en Puebla, México frente a universitarios de Cúcuta, Colombia.Materiales y métodos:Estudio observacional, descriptivo y transversal con 2 grupos de 100 participantes de edad promedio de 20.5±3 y 21±2 años para mexicanos y colombianos respectivamente. Se obtuvo antropometría, signos vitales, condiciones climáticas y realización del test de 20mSRT. Además, se analizó por medio de la prueba no paramétrica de Mann-Whitney y luego ANOVA con prueba post hoc mediante la prueba de Scheffe.Resultados:El 56% (62%Mex. y 50,5%Col.) de los participantes tuvieron un IMC de normopeso, 9,5% (7%Mex. y 6%Col.) infrapeso y para sobrepeso y obesidad fue de 26% (28%Mex. y 24%Col.) y 8,5% (9%Mex. y 8%Col.) respectivamente. Respecto a la circunferencia abdominal se encontró 93,73 ± 11.84 y 81,37 ± 12.20 para universitarios mexicanos y colombianos respectivamente. El VO2máx. en los universitarios mexicanos fue 34.7±6.06 con 672.8±385.4 metros recorridos y en los universitarios colombianos fue de 32.9±7.12 con 533.8±371.3 metros. Conclusiones:No se encontraron diferencias significativas en el IMC (p=0,967), circunferencia abdominal (p=0,258), VO2máx. (p=0,089) ni variables hemodinámicascomo frecuencia cardíaca máxima (p=0,344), saturación arterial de oxígeno (p=0,811), TAS (p=0,945) y TAD (p=0,597) post test. En ambos grupos, los resultados de la capacidad aeróbica estuvieron por debajo de la clasificación de “Bueno” u “Excelente” capacidad aeróbica

Seroepidemiological evidence of severe fever with thrombocytopenia syndrome virus infections in wild boars in Nagasaki, Japan

Severe fever with thrombocytopenia syndrome (SFTS) is an emerging disease in East Asia. It is thought that the SFTS virus (SFTSV) circulates between ticks and animals in nature and that the virus is transmitted to humans by tick bites. SFTS is endemic to Nagasaki in western Japan; however, epidemiological information regarding SFTSV in Nagasaki is not known. In this study, we performed SFTSV IgG ELISAs and neutralization antibody assays for a seroepidemiological survey using samples from wild boars captured in six areas of Nagasaki. SFTSV seropositive animals were found in three areas. Our findings provide epidemiological information on the distribution of SFTSV in Nagasaki

The relevance of the formation of a pharmaceutical chemist biologist: an element of social renponsability of universities

Este estudio de tipo exploratorio, transversal y de campo es solo una fase del proyecto de seguimiento de egresados de la licenciatura en Química Farmacéutico Biológica (QFB) de la Facultad de Estudios Superiores “Zaragoza”. Este proyecto permite retroalimentar el plan curricular, además de establecer el lugar que han ocupado los egresados de esta institución en el mercado laboral, además de detectar las principales áreas de oportunidad en campos profesionales no explorados por los egresados. Así como la determinación de los conocimientos, habilidades, actitudes y valores obtenidos durante su formación y que le han sido indispensables en su desarrollo profesional.This study was exploratory, transversal and field is just one phase of the project following graduates of the FES Zaragoza. This project will provide feedback to the curriculum of the race Pharmaceutical Biological Chemistry (QFB), to establish where they have occupied the graduates of this institution in the labor market, in addition to identifying the main areas of opportunity in professional fields unexplored by the graduates. And the determination of the knowledge, skills, attitudes and values obtained during training and to have been indispensable in their professional development