Non-Specific Indication of Microorganisms in Environmental Samples

The review presents an analysis of modern methods and instruments for performing nonspecific detection of pathogenic biological agents in environmental objects. Discussed are technological characteristics of application of these methods for the detection of biological substances of protein nature in samples. The spectrum of means for non-specific PBA detection includes home-produced and foreign field devices based on protein contamination indication using various colorimetry variants. Technologies for remote and direct monitoring of environment for the presence of aerosols of biological nature are represented by hybrid lidar systems (biolidars) and biodetectors. For PBA nucleic acids tracing, the complexes based on DNA molecule binding with fluorophore with further fluorescence detection are described. Given are the examples of chemiluminescent analysis application in the developed automatic impurity detectors, as well as systems using bioluminescence. Based on the literature data analysis, put forward is a possible algorithm for indication of pathogenic biological agents when carrying out monitoring of the environment in zones of possible emergency situation occurrence and mass events holding

Key Stages in the Development of SAET Laboratory Facilities

Considered are the stages of the SAET laboratory facilities development. Initially main area of activities was assumed to be specific indication of bacterial threats and laboratory control over the ambient environment objects for the presence of particularly dangerous infectious disease agents. Significant increment in the workload occurred during the period of localization and elimination of epidemic cholera manifestations in the 1970s, when primary tasks of bacteriological unit consisted in carrying out mass bacteriological investigations of samples from humans, environment objects, and food items. Assignment of new functions to the laboratory facilities, such as performance of sanitary-microbiological investigations, monitoring over ambient environment objects for the presence of vibrio-flora and natural-focal infectious disease agents, clinical material assays - is associated with SAETs participation in liquidation of medical-sanitary consequences of natural disasters and human cost relief as aftermaths of military conflicts, as well as participation in management of mass events with international representation. Most important issues in the development of SAET laboratory facilities are implementation of advanced diagnostic technologies, automatization of various stages in the process of analysis performing, standardization of diagnostic investigations, and ensuring compliance of the facilities with national and international requirements

К вопросу о современном подходе к диагностике и лечению травм селезенки у детей

 Today, the problem of spleen injury in children still appears relevant. The choice of diagnostics and treatment tactics at the present stage is far from being unified. The priority direction is the organ-preserving approach, which  is possible and justified in children, which significantly reduces surgical  aggression and prevents the likelihood of negative consequences of splenectomy. The choice of the optimal diagnostic method and  determination of safe treatment tactics was the purpose of our study. Thirty-nine injured children were treated in 2007–2019. The predominant cause of spleen injury was fall from height (64.1%). Echography was the main diagnostic method, which was performed around the clock. Splenectomy  was performed in 7 patients (17.9%), in 32 children (82.1%) the organ was preserved. One case (2.6%) was fatal. The analysis of our own results showed that the widespread use of echography, particularly by surgeons, is necessary in the emergency service and greatly simplifies the work. The developed local protocol for diagnosis and treatment is simple and accessible, since it is based on the interpretation of the main criteria, such as systemic hemodynamics and hemoperitoneum. Continued bleeding reasons diagnostic laparoscopy. With unstable hemodynamics, large hemoperitoneum, laparotomy is indicated.Проблема травмы селезенки у детей не теряет своей актуальности и в настоящее время. Выбор диагностики и лечебной тактики на современном этапе далек от унификации. Приоритетным направлением является органосохраняющий подход, который возможен и обоснован у детей, что значительно снижает хирургическую агрессию и предупреждает вероятность отрицательных последствий спленэктомии. Выбор оптимального метода  диагностики и определение безопасной тактики лечения послужили целью нашего исследования. Пролечены 39 пострадавших детей за период с 2007 по 2019 г. Преобладающей причиной повреждения селезенки была кататравма (64,1%). Эхография являлась основным методом диагностики, которую выполняли в круглосуточном режиме.  Спленэктомия проведена 7 пациентам (17,9%), у 32 детей (82,1%) орган сохранен. В одном случае (2,6%) отмечен летальный исход. Анализ собственных результатов показал, что широкое использование эхографии, в том числе и хирургами, необходимо в ургентной службе и значительно упрощает работу. Разработанный локальный протокол диагностики и лечения прост и доступен, так как основывается на интерпретации главных критериев, таких  как системная гемодинамика и гемоперитонеум.  Продолжающееся кровотечение служит основанием для  проведения диагностической лапароскопии. При  нестабильной гемодинамике, большом гемоперитонеуме  показана лапаротомия.

MALDI-TOF Mass-Spectrometry Analysis of Plague Agent Strains

Objective of the study was to demonstrate practicability of data base creation, containing reference mass-spectra of agents of particularly dangerous infections, using MALDI-TOF mass-spectrometry, by the example of plague agent strains. Materials and methods. MALDI-TOF mass-spectrometry was deployed for the obtainment of mass-spectra of ribosomal proteins from the microorganisms under investigation with the help of mass-spectrometers - Microflex LT. Results and conclusions. Carried out was comparative analysis of the obtained mass-spectra of 10 Y. pestis strains and reference spectra of Y. tuberculosis , contained in commercial data base of MALDI Biotyper 3.1 (Bruker Daltonics, Germany). Developed data base was validated in the process of identification of plague microbe strains, isolated in the territory of natural plague foci of the Russian Federation. That data base provided for correct identification of Y. pestis strains up to a species

Management of Diagnostic Investigations at the Premises of Specialized Anti-Epidemic Team Mobile Complex at the Time of Running Mass Events

Laboratory support of epidemiological surveillance plays a significant role in the provision of sanitary-epidemiological welfare of the population at the time of preparations to and carrying out public events. Taking into consideration the increment of load upon the laboratory facilities of the Rospotrebnadzor institutions and general medical-and-prophylactic establishments, there emerges a need to deploy specialized anti-epidemic teams. By the example of management of the laboratory investigations in SAET mobile complex during XXVII World-wide Summer Universiade in Kazan, 2013 and G-20 Summit in Saint-Petersburg, 2013 formulated have been the basic principles of organization and algorithms of diagnostic work at the premises of laboratory facilities of SAET mobile unit deployed in order to provide for sanitary-epidemiological welfare of the population at the time of running public events

Improvement and Standardization of Laboratory Diagnostics Procedures as Concerns Particularly Dangerous, “Emerging”, and “Reemerging” Infectious Diseases

Represented are the data on the key stages of laboratory diagnostics practices standardization as regards particularly dangerous infectious diseases and on the improvement of the normative-regulatory framework on the procedure for organization and carrying out laboratory diagnostics in accordance with three-level system. Worked out are the principles, scheme of indication and identification of pathogenic biological agents of emerging, previously unknown, and atypical microorganisms using modern computerized systems and high-tech equipment

Results of Work of the Rospotrebnadzor SAET on the Provision of Sanitary Epidemiological Welfare of the Population during the Summer Health-Promotion Campaign, 2014 in the Crimean Federal District

Integration of the Crimean Federal District (CFD) into the Russian Federation in March, 2014 predetermined the necessity to organize preventive activities, aimed at the provision of sanitary epidemiological welfare in the region. The paper observes the results of work of the specialized anti-epidemic teams, affiliated to the Stavropol Research Anti-Plague Institute and Russian Research Anti-Plague Institute “Microbe”, on the provision of assistance to CFD Sanitary Epidemiological Service (SES) in operational etiological investigation of infectious diseases outbreaks. Performed is the laboratory analysis of 2407 clinical and environmental samples. 676 tests out of 11518 have showed positive (non-standard) results. Given are the recommendations concerning optimization of work of the CFD SES facilities

Management of the Investigation and Detection of Legionellosis Agent in the Environmental Samples during the Preparation and Holding of the XXII Winter Olympics and XI Paralympics in Sochi

Provided are the data on the management and carrying out of the laboratory control over the hot-water supply systems of the sports venues and accommodation areas for the presence of Legionella pneumophila during the XXII Olympics and XI Paralympics in Sochi, 2014. Inspected have been 105 facilities. The samples from 37 of them showed positive. L. pneumophila DNA concentration varies between 2.19·102 and 3.92·107 genome equivalents (g.e.)/l. By means of bacteriological investigation detected have been legionella colonies in the water supply systems of 16 facilities, at one of the items - the loading is over 1·104 colony-forming units/l. Performed has been comparative analysis of the results obtained using PCR assay and bacteriological test. Based on the genotyping of 7 isolated strains serogroup 1 and 2 strains of 2-14 serogroups it is concluded that L. pneumophila strains circulating in the resort town Sochi are genetically heterogeneous

Management of Work and Procedure for the Laboratory Diagnostics of Infectious Diseases during the Winter XXII Olympics and XI Paralympics, 2014

Analyzed is the management system for laboratory diagnostics of infectious diseases during the XXII Olympics and XI Paralympics, 2014 in Sochi. Reviewed is piece of work, executed in the pre-Olympic period as regards provision of laboratory facilities deployed for clinical material investigation, development of normative and regulatory documentation which considers peculiarities of the situation, identification of diagnostic capacities of the laboratories, and forecasting of the probable volume of laboratory studies by reference to various groups of infections. Put forward is the procedure for arrangement of work at the different stages of laboratory diagnostics, cooperation and response in case of emergency situation in the sphere of sanitary-epidemiological welfare of the population. Discussed is the role of geo-information system and current means of monitoring over epidemiological situation in the participating states and in the region of the Olympics in laboratory support organization

Generalized diffusion processes

Diffusion processes serve as a mathematical model for the physical phenomenon of diffusion. One of the most important problems in the theory of diffusion processes is the development of methods for constructing these processes from a given diffusion matrix and a given drift vector. Focusing on the investigation of this problem, this book is intended for specialists in the theory of random processes and its applications. A generalized diffusion process (that is, a continuous Markov process for which the Kolmogorov local characteristics exist in the generalized sense) can serve as a model for diffusion in a medium moving in a nonregular way. The author constructs generalized diffusion processes under two assumptions: first, that the diffusion matrix is sufficiently regular; and second, that the drift vector is a function integrable to some power, or is a generalized function of the type of the derivative of a measure