Tide and Tidal Current Prediction by High Speed Digital Computer

The Tide and Tidal Current Tables of the U. S. Coast and Geodetic Survey for 1966 have been computed and edited by a digital computer, the IBM 7094. Prediction by this method is found to be more economical and expedient than by the tide prediction machine in use since 1910. The shift to digital predictions has been gradual. The first program was prepared in 1956 to predict hourly tide heights only, for use in storm surge research. The greatest advantage to digital prediction at that time was the elimination of the hour or more required to set up a new problem on the tide predicting machine, when highly accurate predictions were needed for many short periods. Later, as more efficient computers became available, this program was expanded to include the computation of highs and lows, editing the data in a form suitable for publication and the complete prediction and editing of the tidal current tables. The existing program, to a large degree, reproduced the same calculations formerly made on the analogue tide predicting machine, and with comparable accuracy. The greater versatility of this system invites experimentation, not feasible with the analogue computer. Thus, it is expected that in the long run the switch to digital calculations will lead to an increase in the accuracy of the predictions for stations having complex tide problems. The program grew through the years, and is not the most efficient that could be prepared today. Nevertheless, it appears doubtful that the improved efficiency would justify a complete revision. This report gives a general description of the program, the input data specifications and samples of the results

Predictor Equations for Beach Processes and Responses

A stepwise (linear) multiple regression procedure is applied to 11 environmental variables (or predictors) in the beach-ocean-atmosphersey stema t Virginia Beach, Virginia, for the following five predictands: mean longshore current velocity, mean bottom slope in the shoaling-wave zone, average mean grain size in the shoaling-wave zone, and beach deposition and beach erosion on the lower foreshore. Predictors consist of variables related to beach geometry, local water properties, local wind conditions, tidal fluctuations, and wave characteristics The resultant equations are tested against a set of independent data and, with one exception, agree reasonably. It is believed that if the data set were increased to include at least one year\u27s continuous measurements the procedure outlined would yield valid equations for all but stormy-weather conditions.It is presupposed that some provision will have to be made for preconditioning the data, as \u27storm\u27 and \u27nonstorm\u27 data will probably have to be analyzed separately

Kemampuan Pemahaman Konseptual dan Algoritmik Siswa dalam Menyelesaikan Soal-Soal Reaksi Redoks

Tujuan penelitian ini untuk mendeskripsikan kemampuan pemahaman konseptual dan algoritmik siswa dalam menyelesaikan soal-soal reaksi redoks. Penelitian ini dilakukan di dua sekolah yang berada di Kabupaten Gorontalo yaitu di SMAN 1 dan SMAN 2 Limboto. Pengambilan sampel dengan menggunakan teknik simple random sampling,sebanyak 80 siswa dengan objek penelitian siswa yang terdistribusi di SMAN 1 Limboto kelas X MIA4 dan X kelas MIA5 sedangkan di SMAN 2 Limboto kelas X MIA1 dan kelas X MIA2. Kemampuan pemahaman konseptual dan algoritmik siswa diukur dengan menggunakan instrumen tes berbentuk essay. Hasil penelitian menunjukan bahwa kemampuan pemahaman konseptual dan algoritmik siswa dalam menyelesaikan soal-soal reaksi redoks termasuk kategori sangat rendah yaitu sebesar 25,25 % (pemahaman konseptual) dan 12,75 % (pemahaman algoritmik)

A unique small cell lung carcinoma disease progression model shows progressive accumulation of cancer stem cell properties and CD44 as a potential diagnostic marker

OBJECTIVES: Cancer stem cells (CSCs) have been implicated in disease progression of aggressive cancers including small cell lung carcinoma (SCLC). Here, we have examined the possible contribution of CSCs to SCLC progression and aggressiveness. MATERIALS AND METHODS: GLC-14, GLC-16 and GLC-19 SCLC cell lines derived from one patient, representing increasing progressive stages of disease were used. CSC marker expressions was determined by RT-qPCR and western blotting analyses, and heterogeneity was studied by CSC marker expression by immunofluorescence microscopy and flow cytometry. Colony formation assays were used to assess stem cell properties and therapy sensitivity. RESULTS: Increasing expression of stem cell markers MYC, SOX2 and particularly CD44 were found in association with advancing disease. Single and overlapping expression of these markers indicated the presence of different CSC populations. The accumulation of more homogeneous double- and triple-positive CSC populations evolved with disease progression. Functional characterization of CSC properties affirmed higher proficiency of colony forming ability and increased resistance to γ-irradiation in GLC-16 and GLC-19 compared to GLC-14. GLC-19 colony formation was significantly inhibited by a human anti-CD44 antibody. CONCLUSION: The progressive increase of MYC, SOX2 and particularly CD44 expression that was accompanied with enhanced colony forming capacity and resistance in the in vitro GLC disease progression model, supports the potential clinical relevance of CSC populations in malignancy and disease relapse of SCLC

Use of anti-snake venom and clinical outcomes in snake envenomation: a prospective observational study

Background: The only effective measure to prevent or reverse most of the manifestations of venomous snake-bite is timely administration of antisnake venom (ASV) with or without adjunctive treatment as necessary in each case. But recently several concerns have been raised with regard to use of polyvalent ASV. Hence the present study was conducted to assess use of ASV, early adverse reactions to ASV, adjunctive treatment and clinical outcomes in snake-bite patients, which would help to identify areas of problem and provide basis for planning strategies to increase rational use of drugs.Methods: It was a prospective observational study approved by Institutional Ethics Committee. All indoor patients with systemic manifestations of snake envenomation were included in the study. All participants gave written informed consent. Data was obtained prospectively using a structured case record form. Descriptive statistics was used to express the results.Results: Among 52 patients, snake-bite predominantly affected males (59.62%) than females (40.38%). The most common site of snake-bite was lower limb (65.38%). The main indication for ASV administration was vasculotoxic snake-bite (59.62%). Mean dose of ASV use was 18.21±15.51 vials. Mortality was seen in one patient. Majority of patients (28/52, 53.85%) received ASV in the range of 1-10 vials for the management of snake-bite.Conclusions: The use and dose of ASV was appropriate in vasculotoxic snake-bite while few neurotoxic snake-bite patients needed higher than recommended dose.

Experimental evaluation of analgesic activity of PPAR γ agonists: pioglitazone and rosiglitazone

Background: To evaluate analgesic activity of pioglitazone and rosiglitazone by tail flick method in rats and acetic acid induced writhing method in mice.Methods: Albino wistar rats of either sex weighing 180-200 g and Swiss mice weighing 25-30 g were used. Study was conducted after approval from the Institutional Animal Ethics Committee. The tail flick method in rats described by D’Amour and Smith (1941) and acetic acid induced writhing in mice were used. The dose of pioglitazone and rosiglitazone were 20 mg/kg and 10 mg/kg respectively.Results: In tail flick method of analgesia, both, pioglitazone and rosiglitazone have analgesic activity which was statistically comparable to aspirin. In acetic acid induced writhing model of analgesia, the action of pioglitazone and rosiglitazone was significantly greater than the control group but it was less when compared to aspirin.Conclusions: Analgesic activity of pioglitazone and rosiglitazone was comparable to aspirin in tail flick model of analgesia in rats while it was significantly less when compared to tramadol. Analgesic activity of pioglitazone and rosiglitazone was significantly less than aspirin in acetic acid induced writhing method

Inflammasomes are important mediators of prostatic inflammation associated with BPH

Background: There is mounting evidence to support the role of inflammation in benign prostate hyperplasia (BPH), and a recent study reported expression of inflammasome derived cytokine IL-18 in prostate biopsy of BPH patients. Here we examined the expression of inflammasome-derived cytokines and activation of nucleotide-binding oligomerization domain-like receptor with pyrin domain protein 1 (NLRP) 1 inflammasome in a rat model of prostatic inflammation relevant to BPH. Methods: Prostatic inflammation was experimentally induced in three-month-old male Sprague-Dawley rats by intraprostatic injection (50 μL) of either 5 % formalin or saline (sham) into the ventral lobes of prostate. 7 days later, prostate and bladder tissue was harvested for analysis of inflammasome by Western blot, immunohistochemistry and downstream cytokine production by Milliplex. Results: Expression of interleukins, CXC and CC chemokines were elevated 2-15 fold in formalin injected prostate relative to sham. Significant expression of NLRP1 inflammasome components and caspase-1 in prostate were associated with significant elevation of pro and cleaved forms of IL-1β (25.50 ± 1.16 vs 3.05 ± 0.65 pg/mg of protein) and IL-18 (1646.15 ± 182.61 vs 304.67 ± 103.95 pg/mg of protein). Relative to prostate tissue, the cytokine expression in bladder tissue was much lower and did not involve inflammasome activation. Conclusions: Significant upregulation of NLRP1, caspase-1 and downstream cytokines (IL-18 and IL-1β) suggests that a NLRP1 inflammasome is assembled and activated in prostate tissue of this rat model. Recapitulation of findings from human BPH specimens suggests that the inflammasome may perpetuate the inflammatory state associated with BPH. Further clarification of these pathways may offer innovative therapeutic targets for BPH-related inflammation

Role of Viral Hemorrhagic Septicemia Virus Matrix (M) Protein in Suppressing Host Transcription

ABSTRACT Viral hemorrhagic septicemia virus (VHSV) is a pathogenic fish rhabdovirus found in discrete locales throughout the Northern Hemisphere. VHSV infection of fish cells leads to upregulation of the host's virus detection response, but the virus quickly suppresses interferon (IFN) production and antiviral gene expression. By systematically screening each of the six VHSV structural and nonstructural genes, we identified matrix protein (M) as the virus' most potent antihost protein. Only M of VHSV genotype IV sublineage b (VHSV-IVb) suppressed mitochondrial antiviral signaling protein (MAVS) and type I IFN-induced gene expression in a dose-dependent manner. M also suppressed the constitutively active simian virus 40 (SV40) promoter and globally decreased cellular RNA levels. Chromatin immunoprecipitation (ChIP) studies illustrated that M inhibited RNA polymerase II (RNAP II) recruitment to gene promoters and decreased RNAP II C-terminal domain (CTD) Ser2 phosphorylation during VHSV infection. However, transcription directed by RNAP I to III was suppressed by M. To identify regions of functional importance, M proteins from a variety of VHSV strains were tested in cell-based transcriptional inhibition assays. M of a particular VHSV-Ia strain, F1, was significantly less potent than IVb M at inhibiting SV40/luciferase (Luc) expression yet differed by just 4 amino acids. Mutation of D62 to alanine alone, or in combination with an E181-to-alanine mutation (D62A E181A), dramatically reduced the ability of IVb M to suppress host transcription. Introducing either M D62A or D62A E181A mutations into VHSV-IVb via reverse genetics resulted in viruses that replicated efficiently but exhibited less cytotoxicity and reduced antitranscriptional activities, implicating M as a primary regulator of cytopathicity and host transcriptional suppression. IMPORTANCE Viruses must suppress host antiviral responses to replicate and spread between hosts. In these studies, we identified the matrix protein of the deadly fish novirhabdovirus VHSV as a critical mediator of host suppression during infection. Our studies indicated that M alone could block cellular gene expression at very low expression levels. We identified several subtle mutations in M that were less potent at suppressing host transcription. When these mutations were engineered back into recombinant viruses, the resulting viruses replicated well but elicited less toxicity in infected cells and activated host innate immune responses more robustly. These data demonstrated that VHSV M plays an important role in mediating both virus-induced cell toxicity and viral replication. Our data suggest that its roles in these two processes can be separated to design effective attenuated viruses for vaccine candidates