80 research outputs found

    Phylogenomic Resolution of the Cetacean Tree of Life Using Target Sequence Capture.

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    The evolution of cetaceans, from their early transition to an aquatic lifestyle to their subsequent diversification, has been the subject of numerous studies. However, while the higher-level relationships among cetacean families have been largely settled, several aspects of the systematics within these groups remain unresolved. Problematic clades include the oceanic dolphins (37 spp.), which have experienced a recent rapid radiation, and the beaked whales (22 spp.), which have not been investigated in detail using nuclear loci. The combined application of high-throughput sequencing with techniques that target specific genomic sequences provide a powerful means of rapidly generating large volumes of orthologous sequence data for use in phylogenomic studies. To elucidate the phylogenetic relationships within the Cetacea, we combined sequence capture with Illumina sequencing to generate data for ∌3200 protein-coding genes for 68 cetacean species and their close relatives including the pygmy hippopotamus. By combining data from >38,000 exons with existing sequences from 11 cetaceans and seven outgroup taxa, we produced the first comprehensive comparative genomic dataset for cetaceans, spanning 6,527,596 aligned base pairs and 89 taxa. Phylogenetic trees reconstructed with maximum likelihood and Bayesian inference of concatenated loci, as well as with coalescence analyses of individual gene trees, produced mostly concordant and well-supported trees. Our results completely resolve the relationships among beaked whales as well as the contentious relationships among oceanic dolphins, especially the problematic subfamily Delphininae. We carried out Bayesian estimation of species divergence times using MCMCTree, and compared our complete dataset to a subset of clocklike genes. Analyses using the complete dataset consistently showed less variance in divergence times than the reduced dataset. In addition, integration of new fossils (e.g., Mystacodon selenensis) indicate that the diversification of Crown Cetacea began before the Late Eocene and the divergence of Crown Delphinidae as early as the Middle Miocene

    Scale-free static and dynamical correlations in melts of monodisperse and Flory-distributed homopolymers: A review of recent bond-fluctuation model studies

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    It has been assumed until very recently that all long-range correlations are screened in three-dimensional melts of linear homopolymers on distances beyond the correlation length Ο\xi characterizing the decay of the density fluctuations. Summarizing simulation results obtained by means of a variant of the bond-fluctuation model with finite monomer excluded volume interactions and topology violating local and global Monte Carlo moves, we show that due to an interplay of the chain connectivity and the incompressibility constraint, both static and dynamical correlations arise on distances r≫Οr \gg \xi. These correlations are scale-free and, surprisingly, do not depend explicitly on the compressibility of the solution. Both monodisperse and (essentially) Flory-distributed equilibrium polymers are considered.Comment: 60 pages, 49 figure

    A white humpback whale (Megaptera novaeangliae) in the Atlantic Ocean, Svalbard, Norway, August 2012

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    A white humpback whale (Megaptera novaeangliae) was observed on several occasions off Svalbard, Norway, during August 2012. The animal was completely white, except for a few small dark patches on the ventral side of its fluke. The baleen plates were light-coloured, but the animal's eyes had normal (dark) colouration. This latter characteristic indicates that the animal was not an albino; it was a leucistic individual. The animal was a full-sized adult and was engaged in “bubble-feeding”, together with 15–20 other humpback whales, each time it was seen. Subsequent to these sightings, polling of the marine mammal science community has resulted in the discovery of two other observations of white humpback whales in the Barents Sea area, one in 2004 and another in 2006; in both cases the observed individuals were adult animals. It is likely that all of these sightings are of the same individual, but there is no genetic or photographic evidence to confirm this suggestion. The rarity of observations of such white individuals suggests that they are born at very low frequencies or that the ontogenetic survival rates of the colour morph are low

    DNA methylation levels in candidate genes associated with chronological age in mammals are not conserved in a long-lived seabird

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    © 2017 De Paoli-Iseppi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Most seabirds do not have any outward identifiers of their chronological age, so estimation of seabird population age structure generally requires expensive, long-term banding studies. We investigated the potential to use a molecular age biomarker to estimate age in short-tailed shearwaters (Ardenna tenuirostris). We quantified DNA methylation in several A. tenuirostris genes that have shown age-related methylation changes in mammals. In birds ranging from chicks to 21 years of age, bisulphite treated blood and feather DNA was sequenced and methylation levels analysed in 67 CpG sites in 13 target gene regions. From blood samples, five of the top relationships with age were identified in KCNC3 loci (CpG66: R2 = 0.325, p = 0.019). In feather samples ELOVL2 (CpG42: R2 = 0.285, p = 0.00048) and EDARADD (CpG46: R2 = 0.168, p = 0.0067) were also weakly correlated with age. However, the majority of markers had no clear association with age (of 131 comparisons only 12 had a p-value < 0.05) and statistical analysis using a penalised lasso approach did not produce an accurate ageing model. Our data indicate that some age-related signatures identified in orthologous mammalian genes are not conserved in the long-lived short tailed shearwater. Alternative molecular approaches will be required to identify a reliable biomarker of chronological age in these seabirds

    Comparing genotyping algorithms for Illumina's Infinium whole-genome SNP BeadChips

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    The Brassica napus 60K Illumina Infiniumℱ SNP array has had huge international uptake in the rapeseed community due to the revolutionary speed of acquisition and ease of analysis of this high-throughput genotyping data, particularly when coupled with the newly available reference genome sequence. However, further utilization of this valuable resource can be optimized by better understanding the promises and pitfalls of SNP arrays. We outline how best to analyze Brassica SNP marker array data for diverse applications, including linkage and association mapping, genetic diversity and genomic introgression studies. We present data on which SNPs are locus-specific in winter, semi-winter and spring B. napus germplasm pools, rather than amplifying both an A-genome and a C-genome locus or multiple loci. Common issues that arise when analyzing array data will be discussed, particularly those unique to SNP markers and how to deal with these for practical applications in Brassica breeding applications

    Assessing diagnostic applicability of heat release characteristics determined based on ship engine indicator diagrams

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    In order to determine indicator diagrams-based heat release characteristics, a single- zone model of net heat release was used for perfect gas. It was proved that when a constant value for isentropic exponent is assumed, the error in determining the characteristics can be limited to 1% at the nominal load. The effect of errors in determining the position of piston TDC, as well as that of gas passages and indicator valves on the calculated characteristics was evaluated. It was shown that for low-speed engines the effect of gas passages in negligible, while for medium-speed engines the characteristics reveal some deformations (waves), which are repeatable for an individual cylinder in the examined engine construction. The results of the performed investigations and analyses suggest possibility and advisability of the use of heat release characteristics in diagnosing ship engines, in particular low-speed machines
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