39 research outputs found
Evaluation process, relegation and weeding in the Maimonides library, University of C贸rdoba
La gesti贸n de la colecci贸n necesita actualmente de herramientas y acciones que
contribuyan a su evaluaci贸n constante. El objetivo es el de conseguir una colecci贸n
relevante adapt谩ndose a las necesidades de los usuarios. Entre aqu茅llas destaca el
expurgo.
Se analizan aqu铆 los procesos de evaluaci贸n, relegaci贸n y expurgo de la Biblioteca
Maim贸nides de la Universidad de C贸rdoba, los factores que determinaron
su implantaci贸n, su periodicidad, la intervenci贸n secuencial de los servicios implicados,
calendario y objetivos. Todas las acciones se enmarcan en un proceso de
autorregulaci贸n de la colecci贸n y evaluaci贸n constante de la misma.
Se exponen las caracter铆sticas de la colecci贸n de la biblioteca, su origen, evoluci贸n,
el fondo bibliogr谩fico sometido al proceso y las excepciones contempladas.
Se analizan asimismo las acciones previas a la sistematizaci贸n del proceso, realizadas
mediante la donaci贸n de duplicados, implementadas en la biblioteca desde su
creaci贸n.
Los resultados obtenidos son considerados un instrumento b谩sico en la gesti贸n
de la colecci贸n: actualizaci贸n de la colecci贸n de Libre Acceso, liberaci贸n de espacios,
reestructuraci贸n de los mismos, as铆 como de secciones, y mejora de reubicaci贸n
de los fondos bibliogr谩ficos.Currently, the collection management needs a set of tools and actions that contribute
to its ongoing evaluation. Its aim is to achieve a significant collection adapted
to the users needs. Among the highlighted actions we emphasize the weeding.
We analyze the evaluation process, relegation and weeding in the Maimonides
Library, University of C贸rdoba, the factors that determine their location, their periodicity,
sequential intervention, services involved, timing and objectives. All actions
are part of a self-regulatory process of the collection and ongoing evaluation of it.
We expose the characteristics of Maimonides library鈥檚 collections, their origin,
their evolution, the library holdings under the weeding and the considered exceptions.
Furthermore, we develop an analysis of the previous actions, to the implementation
of the process, which consisted in the duplicated book鈥檚 donations. These
actions were put into practice since the library creation.
The results are considered a basic tool in management collection, such as the
update of the collection of free access, freedom of space, restructuring them, as well
as sections, recolocation and improvement of library collections
Fixation of bioactive compounds to the cuticle of Artemia
Artemia is extensively used in aquaculture to feed early stages of cultured marine species. A problem associated
with this practice is that Artemia fails to supply some essential nutrients. As a possible solution, we have devised
a procedure to make Artemia a vehicle for exogenous nutrients and other bioactive compounds. It consists of the
construction of chimeric proteins composed of a chitin-binding domain, which binds to the cuticle of Artemia,
and a carrier domain that conveys a functional property. As confirmatory examples, we describe the successful
fixation to Artemia's metanauplii of two hybrid proteins: a 尾-galactosidase from the thermophilic bacterium
Thermotoga maritima and the jellyfish green fluorescent protein (GFP), both linked to the CBM2 chitin-binding
domain from the hyperthermophilic archaeon Pyrococcus furiosus. Positive results of experiments carried out ex
vivo and in vivo show the validity of this approach. The methodology used could become a general procedure for
the attachment of different kinds of bioactive compounds, such as enzymes, hormones, antibiotics, etc., to the
cuticle of Artemia as well as other arthropods.
Statement of relevance: Our results overcome shortcomings of Artemia as a feedstock.En prensa2,04
Design and baseline characteristics of the finerenone in reducing cardiovascular mortality and morbidity in diabetic kidney disease trial
Background: Among people with diabetes, those with kidney disease have exceptionally high rates of cardiovascular (CV) morbidity and mortality and progression of their underlying kidney disease. Finerenone is a novel, nonsteroidal, selective mineralocorticoid receptor antagonist that has shown to reduce albuminuria in type 2 diabetes (T2D) patients with chronic kidney disease (CKD) while revealing only a low risk of hyperkalemia. However, the effect of finerenone on CV and renal outcomes has not yet been investigated in long-term trials.
Patients and Methods: The Finerenone in Reducing CV Mortality and Morbidity in Diabetic Kidney Disease (FIGARO-DKD) trial aims to assess the efficacy and safety of finerenone compared to placebo at reducing clinically important CV and renal outcomes in T2D patients with CKD. FIGARO-DKD is a randomized, double-blind, placebo-controlled, parallel-group, event-driven trial running in 47 countries with an expected duration of approximately 6 years. FIGARO-DKD randomized 7,437 patients with an estimated glomerular filtration rate >= 25 mL/min/1.73 m(2) and albuminuria (urinary albumin-to-creatinine ratio >= 30 to <= 5,000 mg/g). The study has at least 90% power to detect a 20% reduction in the risk of the primary outcome (overall two-sided significance level alpha = 0.05), the composite of time to first occurrence of CV death, nonfatal myocardial infarction, nonfatal stroke, or hospitalization for heart failure.
Conclusions: FIGARO-DKD will determine whether an optimally treated cohort of T2D patients with CKD at high risk of CV and renal events will experience cardiorenal benefits with the addition of finerenone to their treatment regimen.
Trial Registration: EudraCT number: 2015-000950-39; ClinicalTrials.gov identifier: NCT02545049
Purification and characterization of a Bacillus polymyxa beta-glucosidase expressed in Escherichia coli.
The beta-glucosidase encoded by the bglA gene from Bacillus polymyxa was overproduced in Escherichia coli by using a plasmid in which bglA is under control of the lacI promoter. Induction with isopropyl-beta-D-thiogalactopyranoside allowed an increase in the specific activity of the enzyme of about 100 times the basal level of gene expression. The enzyme was purified by a two-step procedure involving salting out with ammonium sulfate and ion-exchange chromatography with DEAE-cellulose. Fractions of beta-glucosidase activity recovered by this procedure, after electrophoresis in an acrylamide gel and staining with silver nitrate, yielded a single band of protein. This band was shown by a zymogram to correspond to beta-glucosidase activity. The purified protein showed an apparent molecular mass of 50 kDa and an isoelectric point of 4.6, values in agreement with those expected from the nucleotide sequence of the gene. Km values of the enzyme, with either cellobiose or p-nitrophenyl-beta-D-glucoside as the substrate, were determined. It was shown that the enzyme is competitively inhibited by glucose. The effects of different metallic ions and other agents were studied. Hg2+ was strongly inhibitory, while none of the other cations tested had any significant effect. Ethanol did not show the stimulating effect observed with other beta-glucosidases. The mechanism of enzyme action was investigated. High-pressure liquid chromatography analysis with cellobiose as the substrate confirmed previous data revealing the formation of two products, glucose and another, unidentified, compound. Results presented here indicate that this compound is cellotriose formed by transglycosylation
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The legacy of past human land use in current patterns of mammal distribution
Multiple environmental factors are known to shape species distributions at the global scale, including climate and topography, but understanding current extents of occurrence and biodiversity patterns requires considering anthropogenic factors as well. Numerous studies have explored the relationship between contemporary human activities and different biodiversity metrics, but the influence of past activities, such as land-use, remains poorly understood despite being one of the oldest human impacts. Here we evaluate the role of past land-use modifications in the current distribution and conservation status of mammals worldwide using spatial data characterizing human land use from c.B.C.6000 to c.A.D.2000. First, we applied a clustering method that revealed three generalized past human land-use trajectories that represent low-, recently- and steadily-used areas widely represented across the globe. Second, we fitted boosted regression trees to predict total and threatened mammalian richness, globally and within trajectory-clusters, testing the role of environmental factors and multiple human land-use metrics reflecting: total used area at different time spans, rates of land-use change, and the occurrence of remarkable land-use shifts. Environmental factors were identified as the main correlates of current mammalian richness, but several proposed metrics of past land-use were also relevant predictors. Overall, these results highlight the likely existence of a land-use legacy in some regions of the world that has influenced the distribution of extant mammals, particularly of those currently classified as threatened. Even if we cannot change that legacy, our results show that we need to account for past human impacts to understand present biodiversity patterns and, arguably, to guide future actions