T Cell Phenotype and T Cell Receptor Repertoire in Patients with Major Depressive Disorder

While a link between inflammation and the development of neuropsychiatric disorders, including major depressive disorder (MDD) is supported by a growing body of evidence, little is known about the contribution of aberrant adaptive immunity in this context. Here, we conducted in-depth characterization of T cell phenotype and T cell receptor (TCR) repertoire in MDD. For this cross- sectional case–control study, we recruited antidepressant-free patients with MDD without any somatic or psychiatric comorbidities (n = 20), who were individually matched for sex, age, body mass index, and smoking status to a non-depressed control subject (n = 20). T cell phenotype and repertoire were interrogated using a combination of flow cytometry, gene expression analysis, and next generation sequencing. T cells from MDD patients showed significantly lower surface expression of the chemokine receptors CXCR3 and CCR6, which are known to be central to T cell differentiation and trafficking. In addition, we observed a shift within the CD4+ T cell compartment characterized by a higher frequency of CD4+CD25highCD127low/− cells and higher FOXP3 mRNA expression in purified CD4+ T cells obtained from patients with MDD. Finally, flow cytometry-based TCR Vβ repertoire analysis indicated a less diverse CD4+ T cell repertoire in MDD, which was corroborated by next generation sequencing of the TCR β chain CDR3 region. Overall, these results suggest that T cell phenotype and TCR utilization are skewed on several levels in patients with MDD. Our study identifies putative cellular and molecular signatures of dysregulated adaptive immunity and reinforces the notion that T cells are a pathophysiologically relevant cell population in this disorder

The blood-brain barrier is dysregulated in COVID-19 and serves as a CNS entry route for SARS-CoV-2.

Neurological complications are common in COVID-19. Although SARS-CoV-2 has been detected in patients' brain tissues, its entry routes and resulting consequences are not well understood. Here, we show a pronounced upregulation of interferon signaling pathways of the neurovascular unit in fatal COVID-19. By investigating the susceptibility of human induced pluripotent stem cell (hiPSC)-derived brain capillary endothelial-like cells (BCECs) to SARS-CoV-2 infection, we found that BCECs were infected and recapitulated transcriptional changes detected in vivo. While BCECs were not compromised in their paracellular tightness, we found SARS-CoV-2 in the basolateral compartment in transwell assays after apical infection, suggesting active replication and transcellular transport of virus across the blood-brain barrier (BBB) in vitro. Moreover, entry of SARS-CoV-2 into BCECs could be reduced by anti-spike-, anti-angiotensin-converting enzyme 2 (ACE2)-, and anti-neuropilin-1 (NRP1)-specific antibodies or the transmembrane protease serine subtype 2 (TMPRSS2) inhibitor nafamostat. Together, our data provide strong support for SARS-CoV-2 brain entry across the BBB resulting in increased interferon signaling

Kurzzeitmessung: Gtom - Energetische Analyse von Gebäudehüllen

Erprobung und Entwicklung von Messverfahren, die dazu dienen sollen, in kurzer Zeit die bauwerksindividuellen geometrischen, physikalischen und strukturellen Eigenschaften eines Gebäudes zu erfassen. Auf diese Weise soll zu überschaubaren Kosten eine wesentlich besser auf das individuelle Bauwerk angepasste Sanierungsplanung durchgeführt werden können. Perspektivisch können dieselben Messverfahren auch zur Qualitätskontrolle nach Sanierungen und bei Neubauten eingesetzt werden

Remote sensing techniques for building models and energy performance studies of buildings

Setting up building and district models to perform energetic simulations requires detailed information about the existing buildings. For large parts of the building stock accurate plans are missing, incomplete or outdated. Therefore, gathering the required information is a time consuming and expensive task. Remote sensing techniques provide us with fast and increasingly cheap tools to obtain important input data for building and district models. Obtaining geometric information of buildings for whole districts with a resolution of few cm is already state of the art. However, new developments in sensor technology and data processing tools allow us to go significantly beyond this. We can extract facade elements, determine building materials, and estimate heat flows through building envelopes for complete districts. Current developments point towards an even more detailed, quantitative determination of the outer and inner structure of building envelopes and their physical characteristics. These methods use new RGB, infrared, and hyperspectral camera systems, ground- and satellite-based microwave sensing, and combined analyses with various sensors. The rapid developments and decreasing prices for sensors and their carriers (e.g. UAS) make our approaches attractive for practical use. In this paper we provide a review of recent techniques and their application on the building stock at the single-building and district scale. We also give an overview of new developments and an outlook to the future

Data_Sheet_1.PDF

<p>While a link between inflammation and the development of neuropsychiatric disorders, including major depressive disorder (MDD) is supported by a growing body of evidence, little is known about the contribution of aberrant adaptive immunity in this context. Here, we conducted in-depth characterization of T cell phenotype and T cell receptor (TCR) repertoire in MDD. For this cross-sectional case–control study, we recruited antidepressant-free patients with MDD without any somatic or psychiatric comorbidities (n = 20), who were individually matched for sex, age, body mass index, and smoking status to a non-depressed control subject (n = 20). T cell phenotype and repertoire were interrogated using a combination of flow cytometry, gene expression analysis, and next generation sequencing. T cells from MDD patients showed significantly lower surface expression of the chemokine receptors CXCR3 and CCR6, which are known to be central to T cell differentiation and trafficking. In addition, we observed a shift within the CD4⁺ T cell compartment characterized by a higher frequency of CD4⁺CD25^highCD127^low/− cells and higher FOXP3 mRNA expression in purified CD4⁺ T cells obtained from patients with MDD. Finally, flow cytometry-based TCR Vβ repertoire analysis indicated a less diverse CD4⁺ T cell repertoire in MDD, which was corroborated by next generation sequencing of the TCR β chain CDR3 region. Overall, these results suggest that T cell phenotype and TCR utilization are skewed on several levels in patients with MDD. Our study identifies putative cellular and molecular signatures of dysregulated adaptive immunity and reinforces the notion that T cells are a pathophysiologically relevant cell population in this disorder.</p