Prediction of the clinical outcome in invasive candidiasis patients based on serum anti-candida antibody profiles

Comunicaciones a congreso

Serologic profiling of antibody response for prediction fo the clinical outcome in invasive candidiasis

Comunicaciones a congreso

Quality classification of Spanish olive oils by untargeted gas chromatography coupled to hybrid quadrupole–time of flight mass spectrometry with atmospheric pressure chemical ionization and metabolomics-based statistical approach

The novel atmospheric pressure chemical ionization (APCI) source has been used in combination with gas chromatography (GC) coupled to hybrid quadrupole time-of-flight (QTOF) mass spectrometry (MS) for determination of volatile components of olive oil, enhancing its potential for classification of olive oil samples according to their quality using a metabolomics-based approach. The full-spectrum acquisition has allowed the detection of volatile organic compounds (VOCs) in olive oil samples, including extra virgin, virgin and lampante qualities. A dynamic headspace extraction with cartridge solvent elution was applied. The metabolomics strategy consisted of three different steps: a full mass spectral alignment of GC-MS data using MzMine 2.0, a multivariate analysis using Ez-Info and the creation of the statistical model with combinations of responses for molecular fragments. The model was finally validated using blind samples, obtaining an accuracy in oil classification of 70 % taking the official established method, “PANEL TEST” as reference.The authors acknowledge the financial support of Generalitat Valenciana, as research group of excellence (PROMETEO II/2014/023) and Collaborative Research on Environment and Food-Safety (ISIC/2012/016). This work and C. Sales grant has been supported by Universitat Jaume I research promotion plans (P1-1B2013-70

Mapping of mechanical properties at microstructural length scale in WC-Co cemented carbides: Assessment of hardness and elastic modulus by means of high speed massive nanoindentation and statistical analysis

This paper studies the correlation between the microstructure and the mechanical properties at the nanometric length scale of individual WC grains as well as the metallic cobalt binder in cemented carbide systems. The local crystallographic orientation has been determined by electron backscattered diffraction and the microstructural analysis has been performed using field emission scanning electron microscopy. Small-scale hardness and elastic modulus have been assessed by means of high speed massive nanoindentation and subsequent statistical analysis. The attained mechanical property mappings present a clear correlation between local hardness and stiffness with chemical nature for each constitutive phase as well as with the crystallographic orientation for the WC particles. Besides expected findings associated with individual phases, such as clear anisotropy of the ceramic phase (basal plane being harder and stiffer than the prismatic one) and relatively high flow stress for constrained binder, the protocol implemented provides novel information on local mechanical response at interfaces between ceramic particles with different orientations as well as regions within the metallic cobalt binder close to the WC-Co interface.Peer ReviewedPreprin

Prion Protein Gene Variability in Spanish Goats. Inference through Susceptibility to Classical Scrapie Strains and Pathogenic Distribution of Peripheral PrPsc

Classical scrapie is a neurological disorder of the central nervous system (CNS) characterized by the accumulation of an abnormal, partially protease resistant prion protein (PrPsc) in the CNS and in some peripheral tissues in domestic small ruminants. Whereas the pathological changes and genetic susceptibility of ovine scrapie are well known, caprine scrapie has been less well studied. We report here a pathological study of 13 scrapie-affected goats diagnosed in Spain during the last 9 years. We used immunohistochemical and biochemical techniques to discriminate between classical and atypical scrapie and bovine spongiform encephalopathy (BSE). All the animals displayed PrPsc distribution patterns and western blot characteristics compatible with classical scrapie. In addition, we determined the complete open reading frame sequence of the PRNP in these scrapie-affected animals. The polymorphisms observed were compared with those of the herd mates (n¿=¿665) and with the frequencies of healthy herds (n¿=¿581) of native Spanish goats (Retinta, Pirenaica and Moncaina) and other worldwide breeds reared in Spain (Saanen, Alpine and crossbreed). In total, sixteen polymorphic sites were identified, including the known amino acid substitutions at codons G37V, G127S, M137I, I142M, H143R, R151H, R154H, R211Q, Q222K, G232W, and P240S, and new polymorphisms at codons G74D, M112T, R139S, L141F and Q215R. In addition, the known 42, 138 and 179 silent mutations were detected, and one new one is reported at codon 122. The genetic differences observed in the population studied have been attributed to breed and most of the novel polymorphic codons show frequencies lower than 5%. This work provides the first basis of polymorphic distribution of PRNP in native and worldwide goat breeds reared in Spain

Patients’ experiences of lupus related foot problems : a qualitative investigation

Background: Systemic lupus erythematosus (SLE) can present with a variety of symptoms. Previous research has shown there is a high prevalence of lower limb and foot problems in patients with SLE associated with the musculoskeletal, vascular and neurological changes. Furthermore, there is a high prevalence of infections affecting the feet and a range of common skin and nail problems. However, it is not known how these foot problems impact upon people’s lives. Therefore, we aimed to explore this using a qualitative approach. Method: Following ethical approval, 12 participants were recruited who had a diagnosis of SLE, current and/or past experience of foot problems and were over 18 years in age. Following consent, interviews were carried out with an interpretivist phenomenological approach to both data collection and analysis. Results: Seven themes provide insight into: foot problems and symptoms; the impact of these foot problems and symptoms on activities; disclosure and diagnosis of foot problems; treatment of foot problems and symptoms; perceived barriers to professional foot care; unanswered questions about feet and foot care; and identification of the need for professional foot care and foot care advice. Conclusion: These participants tend to “self-treat” rather than disclose that they may need professional foot care. A lack of focus upon foot health within a medical consultation is attributed to the participant’s belief that it is not within the doctor’s role, even though it is noted to contribute to reduced daily activity. There is a need for feet to be included as a part of patient monitoring and for foot health management to be made accessible for people with SLE

Técnicas de ahorro de sangre en cirugía ortopédica

La cirugía ortopédica está asociada con frecuencia a la necesidad de transfusión de sangre homóloga, con los consiguientes riesgos que ello conlleva. En la actualidad se resalta la importancia de la disminución tanto de la transfusión como de la sobretransfusión en este tipo de cirugía. Dicho objetivo se puede alcanzar con el empleo de diversas técnicas de ahorro de sangre, que incluyan la potenciación del uso de sangre antóloga de métodos para disminuir el sangrado perioperatorio. La máxima eficacia para disminuir la necesidad de transfusión de sangre homóloga se deriva de la combinación de varias técnicas de ahorro, como son los programas de autotransfusión, la hipotensión controlada y el empleo de nuevos fármacos como eritropoyetina o aprotinina; todo ello sin olvidar la importancia de establecer unos criterios restrictivos, en consonancia con las directrices más actuales en este sentido, en el momento de indicar la necesidad de una transfusión sanguínea.Technique in orthopaedic surgery requires frequently transfusion of homologous blood which entails several risks. Nowadays, the importance of the decrease of both transfusion and overtransfusion has been proposed for this type of surgery. This aim can be reached by using different blood saving techniques including either the use of antologous blood or methods to minimize perioperative bleeding. The highest efficacy in order to diminish homologous blood transfusion combines several savings techniques as autotransfusion programs, controlled hypotension, and the use of new drugs such as erytropoyetin and aproinin. Restrictive criteria have to be established according to the more recent guidelines for blood transfusion

Comparison of microstructure and mechanical properties at different length scales of WC-Co hardmetals consolidated by binder jetting 3D printing and hip

Additive Manufacturing (AM) is rapidly growing as a revolutionary technique. It provides an interesting ability to produce complex geometries, a key feature for enhancing performance and widening application fields of hardmetal components, limited characteristic for traditional processing techniques. Within this context, it is necessary to study the mechanical integrity of samples produced by AM [AMed] where it is expected to find characteristics linked to the shaping route followed, and study if they will have influence on the final behaviour regarding the mechanical properties expect for a specimen process by a traditional manufacturing route. This work aims to study the final microstructure, mechanical properties and layer assemblage at different length scales for a 12%wtCo– WC grade hardmetals of medium grain size consolidated by binder jetting 3DP and subsequent SinterHIP. In addition, compare this results with the ones obtained from a 12%wtCo–WC specimens process by means of a traditional technique like Hot Isostatic Pressing (HIP) using the same raw material like in the AMed specimens. Hardness Vickers at macro- and microlength scale as well as scratch tests, using different loads and indenter tips, are conducted on both type of samples. The results are analysed and discussed in terms of oriented layer-like material assemblage effects on microstructural variability, mechanical response determined by intrinsic physical behaviour of the material and feedstock used