Effects of BPA on mRNA levels of tryptophan hydroxylase type 1 (Tph1) (panel A) and tryptophan hydroxylase type 2 (Tph2) (panel B) in prefrontal cortex of BPA-treated male and female rats and their respective controls.

<p>* at least p<0.05 vs. their controls.</p

Effects of BPA on protein levels of steroid 5alpha-reductase type 1 (5α-R1) in prefrontal cortex of BPA-treated female rats and controls.

<p>* at least p<0.05 vs. their controls.</p

List of PCR array genes changed by BPA treatment in prefrontal cortex of rats.

<p>List of PCR array genes changed by BPA treatment in prefrontal cortex of rats.</p

Effects of BPA on cytochrome P450 aromatase (P450arom) mRNA levels (panel A) and protein levels (panel B) in prefrontal cortex of BPA-treated male and female rats and their respective controls.

<p>* at least p<0.05 vs. their controls.</p

Effects of BPA on protein levels of tryptophan hydroxylase type 2 (Tph2) in prefrontal cortex of BPA-treated male and female rats and their respective controls.

<p>* at least p<0.05 vs. their controls.</p

Effects of BPA on relative expression of genes involved in neurotoxic responses in prefrontal cortex of male and female rats.

<p>At least p<0.05 vs. their controls.</p

Primer sequences (5′-3′) and PCR conditions for RT-PCR analysis.

<p>Primer sequences (5′-3′) and PCR conditions for RT-PCR analysis.</p

Effects of BPA on mRNA levels of steroid 5α-reductase type 1 (5α-R1) (panel A), 5α-reductase type 2 (5α-R2) (panel B), and 5α-reductase type 3 (5α-R3) (panel C) in prefrontal cortex of BPA-treated male and female rats and their respective controls.

<p>* at least p<0.05 vs. their controls.</p

Western blots for the detection of 5α-Reductase type 1 (5α-R1) (panel A), 5α-Reductase type 2 (5α-R2) (panel B) and aromatase (panel C) in prostate of control and BPA-treated rats at tolerable daily intake (TDI) dose of 50 µg/Kg/d for 4 days.

<p>Equal loading of protein was determined using an anti β-actin antibody. A representative image of three independent experiments is shown for each blot. Normalization for loading differences was achieved by dividing the densitometry values for individual bands by the densitometry values for β-actin in the same lane. Data represent the mean of three independent experiments. ^#P<0.05 vs. Control animals.</p

Plasma testosterone (T) concentration (panel A), estradiol (E2) concentration (panel B), and estradiol/testosterone (E2/T) ratio (panel C) in control and BPA-treated rats at doses of 25, 50, 300, or 600 µg/Kg/d for 4 days.

<p>*P<0.01 vs. Control animals.</p