Internal gravity waves in stratified flows with and without vortical modes

The comprehension of stratified flows is important for geophysical and astrophysical applications. The Weak Wave Turbulence theory aims to provide a statistical description of internal gravity waves propagating in the bulk of such flows. However, internal gravity waves are usually perturbed by other structures present in stratified flow, namely the shear modes and the vortical modes. In order to check whether a weak internal gravity wave turbulence regime can occur, we perform direct numerical simulations of stratified turbulence without shear modes, and with or without vortical modes at various Froude and buoyancy Reynolds numbers. We observe that removing vortical modes naturally helps to have a better overall balance between poloidal kinetic energy, involved in internal gravity waves, and potential energy. However, conversion between kinetic energy and potential energy does not necessarily show fluctuations around zero in our simulations, as we would expect for a system of weak waves. A spatiotemporal analysis reveals that removing vortical modes helps to concentrate the energy around the wave frequency, but it is not enough to observe a weak wave turbulence regime. Yet, we observe that internal gravity waves whose frequency are large compared to the eddy turnover time are present, and we also find evidences for slow internal gravity waves interacting by Triadic Resonance Instabilities in our strongly stratified flows simulations. Finally, we propose conditions that should be fulfilled in order to observe a weak internal gravity waves turbulence regime in real flows.Comment: 27 pages, 17 figure

Wake of inertial waves of a horizontal cylinder in horizontal translation

We analyze theoretically and experimentally the wake behind a horizontal cylinder of diameter $d$ horizontally translated at constant velocity $U$ in a fluid rotating about the vertical axis at a rate $\Omega$. Using particle image velocimetry measurements in the rotating frame, we show that the wake is stabilized by rotation for Reynolds number ${\rm Re}=Ud/\nu$ much larger than in a non-rotating fluid. Over the explored range of parameters, the limit of stability is ${\rm Re} \simeq (275 \pm 25) / {\rm Ro}$, with ${\rm Ro}=U/2\Omega d$ the Rossby number, indicating that the stabilizing process is governed by the Ekman pumping in the boundary layer. At low Rossby number, the wake takes the form of a stationary pattern of inertial waves, similar to the wake of surface gravity waves behind a ship. We compare this steady wake pattern to a model, originally developed by [Johnson, J. Fluid Mech. 120, 359 (1982)], assuming a free-slip boundary condition and a weak streamwise perturbation. Our measurements show a quantitative agreement with this model for ${\rm Ro}\lesssim 0.3$. At larger Rossby number, the phase pattern of the wake is close to the prediction for an infinitely small line object. However, the wake amplitude and phase origin are not correctly described by the weak-streamwise-perturbation model, calling for an alternative model for the boundary condition at moderate rotation rate.Comment: Accepted for publication in Physical Review Fluid

Ultra-structural cell distribution of the melanoma marker iodobenzamide: improved potentiality of SIMS imaging in life sciences

BACKGROUND: Analytical imaging by secondary ion mass spectrometry (SIMS) provides images representative of the distribution of a specific ion within a sample surface. For the last fifteen years, concerted collaborative research to design a new ion microprobe with high technical standards in both mass and lateral resolution as well as in sensitivity has led to the CAMECA NanoSims 50, recently introduced onto the market. This instrument has decisive capabilities, which allow biological applications of SIMS microscopy at a level previously inaccessible. Its potential is illustrated here by the demonstration of the specific affinity of a melanoma marker for melanin. This finding is of great importance for the diagnosis and/or treatment of malignant melanoma, a tumour whose worldwide incidence is continuously growing. METHODS: The characteristics of the instrument are briefly described and an example of application is given. This example deals with the intracellular localization of an iodo-benzamide used as a diagnostic tool for the scintigraphic detection of melanic cells (e.g. metastasis of malignant melanoma). B16 melanoma cells were injected intravenously to C(57)BL(6)/J(1)/co mice. Multiple B16 melanoma colonies developed in the lungs of treated animals within three weeks. Iodobenzamide was injected intravenously in tumour bearing mice six hours before sacrifice. Small pieces of lung were prepared for SIMS analysis. RESULTS: Mouse lung B16 melanoma colonies were observed with high lateral resolution. Cyanide ions gave "histological" images of the cell, representative of the distribution of C and N containing molecules (e.g. proteins, nucleic acids, melanin, etc.) while phosphorus ions are mainly produced by nucleic acids. Iodine was detected only in melanosomes, confirming the specific affinity of the drug for melanin. No drug was found in normal lung tissue. CONCLUSION: This study demonstrates the potential of SIMS microscopy, which allows the study of ultra structural distribution of a drug within a cell. On the basis of our observations, drug internalization via membrane sigma receptors can be excluded

Clinical practice guidelines for BRCA1 and BRCA2 genetic testing

BRCA1 and BRCA2 gene pathogenic variants account for most hereditary breast cancer and are increasingly used to determine eligibility for PARP inhibitor (PARPi) therapy of BRCA-related cancer. Because issues of BRCA testing in clinical practice now overlap with both preventive and therapeutic management, updated and comprehensive practice guidelines for BRCA genotyping are needed. The integrative recommendations for BRCA testing presented here aim to (1) identify individuals who may benefit from genetic counselling and risk-reducing strategies; (2) update germline and tumour-testing indications for PARPi-approved therapies; (3) provide testing recommendations for personalised management of early and metastatic breast cancer; and (4) address the issues of rapid process and tumour analysis. An international group of experts, including geneticists, medical and surgical oncologists, pathologists, ethicists and patient representatives, was commissioned by the French Society of Predictive and Personalised Medicine (SFMPP). The group followed a methodology based on specific formal guidelines development, including (1) evaluating the likelihood of BRCAm from a combined systematic review of the literature, risk assessment models and expert quotations, and (2) therapeutic values of BRCAm status for PARPi therapy in BRCA-related cancer and for management of early and advanced breast cancer. These international guidelines may help clinicians comprehensively update and standardise BRCA testing practices

PRINCIPES ET INDICATIONS DE LA REGENERATION TISSULAIRE GUIDEE (COMPARAISON D'ACTIVITE CLINIQUE APRES 12 MOIS D'ACTIVITE ENTRE LA FACULTE DE MEDECINE DENTAIRE DE QUEBEC ET LE SERVICE D'ODONTOLOGIE DE CLERMONT-FERRAND)

CLERMONT FD-BCIU-Santé (631132104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF