Essential oils as post-harvest crop protectants against the fruit fly drosophila suzukii: Bioactivity and organoleptic profile

The essential oils extracted from mandarin (Citrus reticulata Blanco) fruits, and from tea tree (Maleleuca alternifolia (Maiden and Betche) Cheel) leaves have been chemically analyzed and tested for their bioactivity against D. suzukii. Besides, to estimate consumers’ acceptability of the essential oil (EO) treatments, we evaluated their impact on the organoleptic characteristics of the EO-treated fruits. The main chemical constituents of the two EOs were 1,8-cineole and 4-terpineol for M. alternifolia (22.4% and 17.6% of the total components, respectively), and limonene (83.6% of the total components) for C. reticulata. The behavioral tests indicate that the two EOs are able to deter D. suzukii oviposition and that D. suzukii shows positive chemotaxis to low concentrations of the EOs and negative chemotaxis when the EO concentration increases. While no negative effects on the organoleptic profiles were detected for fruits treated with C. reticulata EO, the olfactory profile of fruits treated with M. alternifolia EO was so negative that they were defined as “not suitable for consumption” by panellists. Overall, our findings indicate that the use of EOs for the post-harvest protection of small fruits is feasible, provided that the essential oils are selected not only for their bioactivity against the insect pest but also for their affinity with the consumers’ sensorial system

SINEUPs: A novel toolbox for RNA therapeutics

RNA molecules have emerged as a new class of promising therapeutics to expand the range of druggable targets in the genome. In addition to 'canonical' protein-coding mRNAs, the emerging richness of sense and antisense long non-coding RNAs (lncRNAs) provides a new reservoir of molecular tools for RNA-based drugs. LncRNAs are composed of modular structural domains with specific activities involving the recruitment of protein cofactors or directly interacting with nucleic acids. A single therapeutic RNA transcript can then be assembled combining domains with defined secondary structures and functions, and antisense sequences specific for the RNA/DNA target of interest. As the first representative molecules of this new pharmacology, we have identified SINEUPs, a new functional class of natural antisense lncRNAs that increase the translation of partially overlapping mRNAs. Their activity is based on the combination of two domains: An embedded mouse inverted SINEB2 element that enhances mRNA translation (effector domain) and an overlapping antisense region that provides specificity for the target sense transcript (binding domain). By genetic engineering, synthetic SINEUPs can potentially target any mRNA of interest increasing translation and therefore the endogenous level of the encoded protein. In this review, we describe the state-of-the-art knowledge of SINEUPs and discuss recent publications showing their potential application in diseases where a physiological increase of endogenous protein expression can be therapeutic

SINEUP non-coding RNA activity depends on specific N6-methyladenosine nucleotides

SINEUPs are natural and synthetic antisense long non-coding RNAs (lncRNAs) selectively enhancing target mRNAs translation by increasing their association with polysomes. This activity requires two RNA domains: an embedded inverted SINEB2 element acting as effector domain, and an antisense region, the binding domain, conferring target selectivity. SINEUP technology presents several advantages to treat genetic (haploinsufficiencies) and complex diseases restoring the physiological activity of diseased genes and of compensatory pathways. To streamline these applications to the clinic, a better understanding of the mechanism of action is needed. Here we show that natural mouse SINEUP AS Uchl1 and synthetic human miniSINEUP-DJ-1 are N6-methyladenosine (m6A) modified by METTL3 enzyme. Then, we map m6A-modified sites along SINEUP sequence with Nanopore direct RNA sequencing and a reverse transcription assay. We report that m6A removal from SINEUP RNA causes the depletion of endogenous target mRNA from actively translating polysomes, without altering SINEUP enrichment in ribosomal subunit-associated fractions. These results prove that SINEUP activity requires an m6A-dependent step to enhance translation of target mRNAs, providing a new mechanism for m6A translation regulation and strengthening our knowledge of SINEUP-specific mode of action. Altogether these new findings pave the way to a more effective therapeutic application of this well-defined class of lncRNAs

Near term perspectives for fusion research and new contributions by the Ignitor program

The main advances made within the Ignitor program, that is aimed at investigating the physics of fusion burning plasmas near ignition, are described. In particular, the operation of the machine in the H and I regimes at the 10 MA plasma current levels has been considered and analyzed. The unique properties of the plasmas that can be generated by operating the machine with reduced parameters (lower magnetic fields and plasma currents) relative to those needed to achieve ignition are identified. A key feature of this operation is the relatively fast duty cycle that can be maintained. The Ideal Ignition Conditions, under which the density barrier due to bremsstrahlung emission in high density plasmas is removed, can be attained in this case. The plasma heating cycles are identified for which the contribution of ICRH is used both to enter the H-regime and to optimize the time needed for ignition. The on going effort to set up a test ICRH facility is described. The initial results (2 km/sec) of the high speed pellet injection system developed for Ignitor and operated at Oak Ridge are reported. The combined structural analysis and integration of the entire machine core (Load Assembly) is discussed. The adopted control system for both the machine and the plasma column has been designed and is described. The design solutions of the vertical field coils made of MgB2 and operating at 10 K have been identified and the relevant R&D program is underway. The analysis of the Caorso site and of its facility for the operation of the Ignitor with approved safety standards is completed. The relevant results are being made available for the operation of Ignitor at the Triniti site within the framework of the Italy-Russia agreement on the joint construction and operation of the Ignitor facility. A development effort concerning the advanced diagnostic systems that is being carried out for fusion burning plasma regimes is described. An initial analysis of the characteristics of a neutron source based on a system of Ignitor-like machines is reported

Installation of the Ignitor Machine at the Caorso Site

The actual cost of building a new experiment can be considerably contained if infrastructures are already available on its envisioned site. The facilities of the Caorso site (near Piacenza, Italy) that, at present, houses a spent nuclear power station, have been analyzed in view of their utilization for the operation of the Ignitor machine. The main feature of the site is its robust connection to the electrical national power grid that can take the disturbance caused by Ignitor discharges with the highest magnetic fields and plasma currents, avoiding the need for rotating flywheels generators. Other assets include a vast building that can be modified to house the machine core and the associated diagnostic systems. A layout of the Ignitor plant, including the tritium laboratory and other service areas, the distribution of the components of the electrical power supply system and of the He gas cooling sytem are presented. Relevant safety issues have been analyzed, based on the in depth activation analysis of the machine components carried out by means of the FISPACT code. Waste management and environment impact issues, including risk to the population assessments, have also been addressed

3D photogrammetric reconstruction by “Structure from Motion” as a monitoring technique for safety, conservation and improvement of the fruition of cultural heritage

In the last years, with the large growth of new state-of-the-art technologies used in the field of cultural heritage, it's increasingly necessary to promote non-invasive and non-destructive techniques which are "sensitive" to the nature of the site in which they are applied, respecting the Italian historical artistic heritage. Within the COBRA project [1], ENEA developed a new low-cost and non-destructive survey technique by 3D Photogrammetric reconstruction, using the Structure from Motion (SfM) method, for innovative applications in the field of cultural heritage [2]. This paper shows an application of this methodology on the archaeological complex of Priscilla's Catacombs in Rome. Three case studies have been identified, with several design purposes, showing the different applications of this technique: a sarcophagus called "Sarcofago delle Muse" , a chapel named "Cappella Greca" and a masonry element, which are all situated in the "Criptoportico" area