SPM to the heart: mapping of 4D continuous velocities for motion abnormality quantification

International audienceThis paper proposes to apply parallel transport and statistical atlas techniques to quantify 4D myocardial motion abnormalities. We take advantage of our previous work on cardiac motion , which provided a continuous spatiotemporal representation of velocities, to interpolate and reorient cardiac motion fields to an unbiased reference space. Abnormal motion is quantified using SPM analysis on the velocity fields, which includes a correction based on random field theory to compensate for the spatial smoothness of the velocity fields. This paper first introduces the imaging pipeline for constructing a continuous 4D velocity atlas. This atlas is then applied to quantify abnormal motion patterns in heart failure patients

HCG18, LEF1AS1 and lncCEACAM21 as biomarkers of disease severity in the peripheral blood mononuclear cells of COVID-19 patients

Background: Even after 3 years from SARS-CoV-2 identification, COVID-19 is still a persistent and dangerous global infectious disease. Significant improvements in our understanding of the disease pathophysiology have now been achieved. Nonetheless, reliable and accurate biomarkers for the early stratification of COVID-19 severity are still lacking. Long noncoding RNAs (LncRNAs) are ncRNAs longer than 200 nucleotides, regulating the transcription and translation of protein-coding genes and they can be found in the peripheral blood, thus holding a promising biomarker potential. Specifically, peripheral blood mononuclear cells (PBMCs) have emerged as a source of indirect biomarkers mirroring the conditions of tissues: they include monocytes, B and T lymphocytes, and natural killer T cells (NKT), being highly informative for immune-related events. Methods: We profiled by RNA-Sequencing a panel of 2906 lncRNAs to investigate their modulation in PBMCs of a pilot group of COVID-19 patients, followed by qPCR validation in 111 hospitalized COVID-19 patients. Results: The levels of four lncRNAs were found to be decreased in association with COVID-19 mortality and disease severity: HLA Complex Group 18-242 and -244 (HCG18-242 and HCG18-244), Lymphoid Enhancer Binding Factor 1-antisense 1 (LEF1-AS1) and lncCEACAM21 (i.e. ENST00000601116.5, a lncRNA in the CEACAM21 locus). Interestingly, these deregulations were confirmed in an independent patient group of hospitalized patients and by the re-analysis of publicly available single-cell transcriptome datasets. The identified lncRNAs were expressed in all of the PBMC cell types and inversely correlated with the neutrophil/lymphocyte ratio (NLR), an inflammatory marker. In vitro, the expression of LEF1-AS1 and lncCEACAM21 was decreased upon THP-1 monocytes exposure to a relevant stimulus, hypoxia. Conclusion: The identified COVID-19-lncRNAs are proposed as potential innovative biomarkers of COVID-19 severity and mortality

Spectral edge image fusion: theory and applications

NoThis paper describes a novel approach to the fusion of multidimensional images for colour displays. The goal of the method is to generate an output image whose gradient matches that of the input as closely as possible. It achieves this using a constrained contrast mapping paradigm in the gradient domain, where the structure tensor of a high-dimensional gradient representation is mapped exactly to that of a low-dimensional gradient field which is subsequently reintegrated to generate an output. Constraints on the output colours are provided by an initial RGB rendering to produce ‘naturalistic’ colours: we provide a theorem for projecting higher-D contrast onto the initial colour gradients such that they remain close to the original gradients whilst maintaining exact high-D contrast. The solution to this constrained optimisation is closed-form, allowing for a very simple and hence fast and efficient algorithm. Our approach is generic in that it can map any N-D image data to any M-D output, and can be used in a variety of applications using the same basic algorithm. In this paper we focus on the problem of mapping N-D inputs to 3-D colour outputs. We present results in three applications: hyperspectral remote sensing, fusion of colour and near-infrared images, and colour visualisation of MRI Diffusion-Tensor imaging