DNA adducts, cell proliferation and papilloma latency time in mouse skin after repeated dermal application of DMBA and TPA

The mouse skin tumor model was used to investigate whether the level of DNA adducts and/or the rate of cell division in the epidermis are indicators of the risk of cancer formation for an individual in an outbred animal population. A high risk was considered to be reflected by a short latency period for the appearance of a papilloma. Female NMRI mice were treated twice weekly with 2.5 nmol 7,12-dimethylbenz[a]anthracene (DMBA) and 3 nmol 12-O-tetradecanoylphorbol-13-acetate (TPA) and the appearance of papillomas was registered. The first papilloma appeared after 7.5 weeks. After 17 weeks, when 12 of 14 mice had at least one papilloma, an osmotic minipump delivering 5-bromo-2'-deoxyuridine (BrdU) was implanted into each mouse for 24 h. The mice were killed after 24 h and the epidermis was analyzed for DMBA-nucleotide adducts by 32P-postlabeling, for the cell number per unit skin length, and for the labeling index for DNA synthesis. Unexpectedly, DMBA-nucleotide adduct levels were highest in those animals which showed the longest latency periods. Adduct levels were negatively correlated with the labeling index, indicating that dilution of adducts by cell division was a predominant factor in determining average adduct concentrations. Individual tumor-latency time was not correlated with either cell number or labeling index. This could be due to the fact that the measurements only provided averaged data and gave no information on the specific situation in clones of premalignant cells. Under the conditions of this assay, therefore, neither DNA adduct levels nor information on the average kinetics of cell division had a predictive value for the individual cancer risk within a group of outbred animals receiving the same treatmen

The psychology of scams: Provoking and committing errors of judgement

According to the Office of Fair Trading (2006), 3.2 million adults in the UK fall victim to mass marketed scams every year, and collectively lose £3.5 billion. Victims of scams are often labelled as 'greedy' or 'gullible' and elicit the reaction, 'How on earth could anyone fall for that?' However, such labels are unhelpful and superficial generalisations that presume all of us are perfectly rational consumers, ignoring the fact that all of us are vulnerable to a persuasive approach at one time or another. Clearly, responding to a scam is an error of judgement – so our research sought to identify the main categories of decision error that typify victim responses, and to understand the psychology of persuasion employed by scammers to try to provoke such errors.UK Office of Fair Tradin

Prenatal exposure to methadone or buprenorphine: Early childhood developmental outcomes.

BACKGROUND: Methadone and buprenorphine are recommended to treat opioid use disorders during pregnancy. However, the literature on the relationship between longer-term effects of prenatal exposure to these medications and childhood development is both spare and inconsistent. METHODS: Participants were 96 children and their mothers who participated in MOTHER, a randomized controlled trial of opioid-agonist pharmacotherapy during pregnancy. The present study examined child growth parameters, cognition, language abilities, sensory processing, and temperament from 0 to 36 months of the child\u27s life. Maternal perceptions of parenting stress, home environment, and addiction severity were also examined. RESULTS: Tests of mean differences between children prenatally exposed to methadone vs. buprenorphine over the three-year period yielded 2/37 significant findings for children. Similarly, tests of mean differences between children treated for NAS relative to those not treated for NAS yielded 1/37 significant finding. Changes over time occurred for 27/37 child outcomes including expected child increases in weight, head and height, and overall gains in cognitive development, language abilities, sensory processing, and temperament. For mothers, significant changes over time in parenting stress (9/17 scales) suggested increasing difficulties with their children, notably seen in increasing parenting stress, but also an increasingly enriched home environment (4/7 scales). CONCLUSIONS: Findings strongly suggest no deleterious effects of buprenorphine relative to methadone or of treatment for NAS severity relative to not-treated for NAS on growth, cognitive development, language abilities, sensory processing, and temperament. Moreover, findings suggest that prenatal opioid agonist exposure is not deleterious to normal physical and mental development

Effect of HIV/AIDS and Malaria on the Context for Introduction of Zinc Treatment and Low-osmolarity ORS for Childhood Diarrhoea*

Diarrhoea was estimated to account for 18% of the estimated 10.6 million deaths of children aged less than five years annually in 2003. Two—Africa and South-East Asia—of the six regions of the World Health Organization accounted for approximately 40% and 31% of these deaths respectively, or almost three-quarters of the global annual deaths of children aged less than five years attributable to diarrhoea. Much of the effort to roll out low-osmolarity oral rehydration solution (ORS) and supplementation of zinc for the management of diarrhoea accordingly is being devoted to sub-Saharan Africa and to South and South-East Asia. A number of significant differences exist in diarrhoea-treatment behaviours and challenges of the public-health systems between Africa and Asia. The differences in rates of ORS use are the most common indicator of treatment of diarrhoea and vary dramatically by and within region and may significantly influence the roll-out strategy for zinc and low-osmolarity ORS. The prevalence of HIV/AIDS and the endemi-city of malaria also differ greatly between regions; both the diseases consume the attention and financial commitment of public-health programmes in regions where rates are high. This paper examined how these differences could affect the context for the introduction of zinc and low-osmolarity ORS at various levels, including the process of policy dialogue with local decision-makers, questions to be addressed in formative research, implementation approaches, and strategies for behaviour-change communication and training of health workers

Silicon Nanowires: Doping Dependent N- And P- Channel FET Behavior

The electrical transport properties of field effect transistor (FET) devices made of silicon nanowires (SiNWs) synthesized by pulsed laser vaporization (PLV) were studied. From as-grown PLV-SiNW FET, we found p-channel FET behavior with low conductance. To improve conductance, spin on glass (SOG) and vapor doping were used to dope phosphorus and indium into SiNW, respectively. From doping after synthesis, we could successfully make both n- and p-channel FET devices

Experimental investigation of the pre–Darcy regime

The validity of Darcy’s law at very low Reynolds numbers is discussed controversially in literature, as some authors propose a pre–Darcy flow regime below some critical Reynolds number. The scope of this work is to investigate this problem experimentally. Therefore, a packing of glass spheres is perfused by different glycerin–water solutions. A linear behaviour between the flow velocity and the pressure drop through the packed spheres is found in the complete investigated range of Reynolds number Red′, based on the mean-pore diameter d′ and mean-pore velocity v′ with 10−9≤Red′≤10−1. This contradicts the results of different authors like Fand et al. (1987) or Kececioglu and Jiang (1994), postulating a pre–Darcy regime for Red′≤2.8⋅10−6 or Red′≤0.13, respectively

Two Jovian-Mass Planets in Earthlike Orbits

We report the discovery of two new planets: a 1.94 M_Jup planet in a 1.8-year orbit of HD 5319, and a 2.51 M_Jup planet in a 1.1-year orbit of HD 75898. The measured eccentricities are 0.12 for HD 5319 b and 0.10 for HD 75898 b, and Markov Chain Monte Carlo simulations based on derived orbital parameters indicate that the radial velocities of both stars are consistent with circular planet orbits. With low eccentricity and 1 < a < 2 AU, our new planets have orbits similar to terrestrial planets in the solar system. The radial velocity residuals of both stars have significant trends, likely arising from substellar or low-mass stellar companions.Comment: 32 pages, including 11 figures and 5 tables. Accepted by Ap

Turning to God in the Face of Ostracism: Effects of Social Exclusion on Religiousness

The present research proposes that individuals who are socially excluded can turn to religion to cope with the experience. Empirical studies conducted to test this hypothesis consistently found that socially excluded persons reported (a) significantly higher levels of religious affiliation (Studies 1, 2, and 4) and (b) stronger intentions to engage in religious behaviors (Study 2) than comparable, nonexcluded individuals. Direct support for the stress-buffering function of religiousness was also found, with a religious prime reducing the aggression-eliciting effects of consequent social rejection (Study 5). These effects were observed in both Christian and Muslim samples, revealing that turning to religion can be a powerful coping response when dealing with social rejection. Theoretical and practical implications of these findings are discussed