507 research outputs found
Accurate Measurement in the Field of the Earth of the General-Relativistic Precession of the LAGEOS II Pericenter and New Constraints on Non-Newtonian Gravity
The pericenter shift of a binary system represents a suitable observable to
test for possible deviations from the Newtonian inverse-square law in favor of
new weak interactions between macroscopic objects. We analyzed 13 years of
tracking data of the LAGEOS satellites with GEODYN II software but with no
models for general relativity. From the fit of LAGEOS II pericenter residuals
we have been able to obtain a 99.8% agreement with the predictions of
Einstein's theory. This result may be considered as a 99.8% measurement in the
field of the Earth of the combination of the {\gamma} and {\beta} parameters of
general relativity, and it may be used to constrain possible deviations from
the inverse-square law in favor of new weak interactions parametrized by a
Yukawa-like potential with strength {\alpha} and range {\lambda}. We obtained
|{\alpha}|\lesssim1\times10-11, a huge improvement at a range of about 1 Earth
radius
An Integrated NMR, LC-DAD-MS, LC-QTOF Metabolomic Characterization of Sartoria hedysaroides: Correlation of Antioxidant and Enzyme Inhibitory Activity with Chemical Composition by Multivariate Data Analysis
Sartoria hedysaroides Boiss and Heldr. (Fabaceae) is an endemic plant of Turkey that has received little scientific consideration so far. In the present study, the chemical profiles of extracts from the aerial part and roots of S. hedysaroides obtained using solvents with different polarities were analyzed combining integrated NMR, LC-DAD-MSn, and LC-QTOF methods. In vitro antioxidant and enzyme inhibitory activities were evaluated, and the results were combined with chemical data using multivariate approaches. Phenolic acids, flavonoids, ellagitannins, and coumarins were identified and quantified in the extracts of aerial part and roots. Methanolic extract of S. hedysaroides aerial part showed the highest phenolic content and the highest antioxidant activity and cupric ion reducing antioxidant capacity. Dichloromethane extract of S. hedysaroides roots showed the highest inhibition of butyryl cholinesterase, while methanolic extract of S. hedysaroides aerial part was the most active tyrosinase inhibitor. Multivariate data analysis allowed us to observe a good correlation between phenolic compounds, especially caffeoylquinic derivatives and flavonoids and the antioxidant activity of extracts. Acetylcholinesterase inhibition was correlated with the presence of caffeoylquinic acids and coumarins. Overall, the present study appraised the biological potential of understudied S. hedysaroides, and provided a comprehensive approach combining metabolomic characterization of plant material and multivariate data analysis for the correlation of chemical data with results from multi-target biological assays
TEPEE/GReAT (General Relativity Accuracy Test in an Einstein Elevator): Ready to start
TEPEE/GReAT is an experiment aimed at testing the principle of equivalence at a level of accuracy equal to 5 parts in 1015 by means of a differential acceleration detector free falling inside a co-moving, cryogenic, evacuated capsule,
released from a stratospheric balloon. The detector is spun about a horizontal axis during the fall in order to modulate the equivalence principle violation signal at the
spin frequency. Thanks to the recent funding of the Italian side, the project is ready to enter its second phase. The main activities related to detector prototype (both
non-cryogenic and cryogenic versions) development and testing, free-fall tests, signal extraction from noise (in particular related to the common-mode rejection factor) and flight model requirements are discussed
Micronucleus formation induced by biomass burning particles derived from biomass burning induce high micronucleus frequency in Tradescantia pallida assay (TRAD-MN)
Manual harvesting is usually done after to sugar cane burning which is responsible for seasonal emission of air pollutants in Brazil and it is believed to be responsible for deleterious health effects in exposed populations. The mutagenic potential of sugar cane burning harvesting particulate and particle surrogates of residual oil fly ash (ROFA) were evaluated in assays measuring micronuclei (MN) in the pollen mother cells of Tradescantia pallida (TRAD-MN). Micronuclei frequencies in TRAD-MN to sugar cane burning residues (SCBR) at doses 0.3 and 0.03 mg/mL were respectively 2.18 ± 0.35 and 5.53 ± 1.04, whereas to ROFA from incinerator and ROFA from an electrostatic precipitator installed in one of the chimneys of a steel plant, MN frequencies were, respectively, 3.43 ± 0.7 and 4.90 ± 1.07. Significant differences were detected among the groups (p < 0.001), demonstrating that SCBR was at least as genotoxic as the fossil fuel derived particles. The results suggest that the burning process to harvest sugar cane should be better controlled. Keywords: pollution, burning particles, micronuclei, Tradescantia pallida . PartÃculas derivadas da queima de biomassa induzem alta frequência de micronúcleos no ensaio de Tradescantia pallida (TRAD-MN) Resumo A coleta de cana-de-açúcar manual é geralmente realizada após a queima, a qual é responsável pela emissão sazonal de poluentes atmosféricos no Brasil e acredita-se que este seja responsável pelos efeitos deletérios a saúde da população exposta. O potencial mutagênico da queima de cana-de-açúcar e partÃculas oriundas de resÃduos em cinza de óleo queimado (ROFA) foi avaliado em ensaio que mede a freqüência de micronúcleos (MN) em células mãe de grão de pólen de Tradescantia pallida (TRAD-MN). A freqüência de TRAD-MN em resÃduos de queima de cana de açúcar (SCBR) nas doses de 0.3 e 0,03mg/mL foi, respectivamente de 2,18 ± 0,35 e 5,53 ± 1,04, enquanto para ROFA de incinerador e ROFA de um precipitador eletrostático instalado em uma chaminé de uma indústria siderúrgica, a freqüência de MN foi, respectivamente, 3,43 ± 0,7 e 4,90 ± 1,07. Diferença significante foi observada entre os grupos (p<0,001), demonstrando que SCBR foi ao menos tão genotóxico quanto à s partÃculas derivadas de combustÃveis fósseis. Os resultados sugerem que o processo de queima de cana-de-açúcar no perÃodo da safra deveria ser mais bem controlado. Palavras-Chave: poluição, partÃculas de queimadas, micronúcleos, Tradescantia pallida
Spectral Theory of Sparse Non-Hermitian Random Matrices
Sparse non-Hermitian random matrices arise in the study of disordered
physical systems with asymmetric local interactions, and have applications
ranging from neural networks to ecosystem dynamics. The spectral
characteristics of these matrices provide crucial information on system
stability and susceptibility, however, their study is greatly complicated by
the twin challenges of a lack of symmetry and a sparse interaction structure.
In this review we provide a concise and systematic introduction to the main
tools and results in this field. We show how the spectra of sparse
non-Hermitian matrices can be computed via an analogy with infinite dimensional
operators obeying certain recursion relations. With reference to three
illustrative examples --- adjacency matrices of regular oriented graphs,
adjacency matrices of oriented Erd\H{o}s-R\'{e}nyi graphs, and adjacency
matrices of weighted oriented Erd\H{o}s-R\'{e}nyi graphs --- we demonstrate the
use of these methods to obtain both analytic and numerical results for the
spectrum, the spectral distribution, the location of outlier eigenvalues, and
the statistical properties of eigenvectors.Comment: 60 pages, 10 figure
The relevance of mitochondrial DNA variants fluctuation during reprogramming and neuronal differentiation of human iPSCs
The generation of inducible pluripotent stem cells (iPSCs) is a revolutionary technique allowing production of pluripotent patient-specific cell lines used for disease modeling, drug screening, and cell therapy. Integrity of nuclear DNA (nDNA) is mandatory to allow iPSCs utilization, while quality control of mitochondrial DNA (mtDNA) is rarely included in the iPSCs validation process. In this study, we performed mtDNA deep sequencing during the transition from parental fibroblasts to reprogrammed iPSC and to differentiated neuronal precursor cells (NPCs) obtained from controls and patients affected by mitochondrial disorders. At each step, mtDNA variants, including those potentially pathogenic, fluctuate between emerging and disappearing, and some having functional implications. We strongly recommend including mtDNA analysis as an unavoidable assay to obtain fully certified usable iPSCs and NPCs
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