2,898 research outputs found
A Computer-Aided Drug Design Approach to Predict Marine Drug-Like Leads for SARS-CoV-2 Main Protease Inhibition
UIDB/50006/2020 UIDB/04378/2020 Norma transitória DL 57/2016The investigation of marine natural products (MNPs) as key resources for the discovery of drugs to mitigate the COVID-19 pandemic is a developing field. In this work, computer-aided drug design (CADD) approaches comprising ligand- and structure-based methods were explored for predicting SARS-CoV-2 main protease (Mpro) inhibitors. The CADD ligand-based method used a quantitative structure–activity relationship (QSAR) classification model that was built using 5276 organic molecules extracted from the ChEMBL database with SARS-CoV-2 screening data. The best model achieved an overall predictive accuracy of up to 67% for an external and internal validation using test and training sets. Moreover, based on the best QSAR model, a virtual screening campaign was carried out using 11,162 MNPs retrieved from the Reaxys® database, 7 in-house MNPs obtained from marine-derived actinomycetes by the team, and 14 MNPs that are currently in the clinical pipeline. All the MNPs from the virtual screening libraries that were predicted as belonging to class A were selected for the CADD structure-based method. In the CADD structure-based approach, the 494 MNPs selected by the QSAR approach were screened by molecular docking against Mpro enzyme. A list of virtual screening hits comprising fifteen MNPs was assented by establishing several limits in this CADD approach, and five MNPs were proposed as the most promising marine drug-like leads as SARS-CoV-2 Mpro inhibitors, a benzo[f]pyrano[4,3-b]chromene, notoamide I, emindole SB beta-mannoside, and two bromoindole derivatives.publishersversionpublishe
Delivering of Proteins to the Plant Vacuole-An Update
Trafficking of soluble cargo to the vacuole is far from being a closed issue as it can occur by different routes and involve different intermediates. The textbook view of proteins being sorted at the post-Golgi level to the lytic vacuole via the pre-vacuole or to the protein storage vacuole mediated by dense vesicles is now challenged as novel routes are being disclosed and vacuoles with intermediate characteristics described. The identification of Vacuolar Sorting Determinants is a key signature to understand protein trafficking to the vacuole. Despite the long established vacuolar signals, some others have been described in the last few years, with different properties that can be specific for some cells or some types of vacuoles. There are also reports of proteins having two different vacuolar signals and their significance is questionable: a way to increase the efficiency of the sorting or different sorting depending on the protein roles in a specific context? Along came the idea of differential vacuolar sorting, suggesting a possible specialization of the trafficking pathways according to the type of cell and specific needs. In this review, we show the recent advances in the field and focus on different aspects of protein trafficking to the vacuoles
Pragas associadas à castanha em Trás-os-Montes: biologia e estragos.
A castanha Ă© uma das principais produções frutĂcolas de Trás-os-Montes, representando um
peso na economia regional, em especial na Terra Fria. O fruto Ă© atacado por algumas pragas e
doenças que depreciam o seu valor comercial e causando perdas no rendimento dos agricultores.
Este trabalho, teve por objectivo proceder a uma estimativa dos estragos provocados por pragas e
doenças da castanha e por outro obter dados acerca da biologia do bichado da castanha,
Laspeyresia (= Cydia) splendana (HĂĽbner), a principal praga deste fruto na regiĂŁo, como primeiro
passo para o delineamento de estratégias adequadas na protecção da castanha. Os estragos foram
variáveis de acordo com o souto e ano, atingindo o máximo de 67,6% de frutos num dos soutos em
2004, sendo na sua maioria originados por pragas. Foram registadas capturas do bichado da
castanha entre inĂcio de Julho e Outubro com um pico marcado em finais de Agosto/inĂcio de
Setembro
Estilos de liderança dos diretores técnicos e satisfação dos colaboradores: estudo realizado em ips's dos distritos da Guarda e Viseu
Introduction: This work is a quantitative, transversal, descriptive and correlational study. Objectivos: The main purpose of the present study was to understand the collaborators perspective of the relationship between the leadership styles used by technical directors and job satisfaction of employees in nine Private Social Solidarity Institutions (PSSI,) in the districts of Guarda and Viseu, taking also into account the relevance of sociodemographic and professional variables in the field of job satisfaction. Methods: The sample consists of 85 employees and the data collection instrument integrates questions of socio-demographic and professional characterization and two validated scales for assessing: i) the degree of job satisfaction in various dimensions (developed by Instituto da Segurança Social, 2007) and ii) the leadership style of the technical directors (developed by Melo, 2004), according to the perception of employees. Results: The results indicate that the relational leadership style promotes greater satisfaction and the leadership focused on task induces lower satisfaction. Conclusions: The hypothesis under study allows to conclude that variables: gender, type of work contract, service time and qualifications influence employee’s job satisfaction.Introdução: Este trabalho trata-se de um estudo quantitativo, transversal, descritivo e correlacional. Objetivos: O presente estudo teve como principal objetivo conhecer, na perspetiva dos colaboradores, a relação entre os estilos de liderança utilizados pelos diretores tĂ©cnicos e a satisfação dos colaboradores em nove IPSS`s dos distritos da Guarda e Viseu, atendendo ainda Ă relevância de variáveis sociodemográficas e profissionais no âmbito da satisfação no trabalho. MĂ©todos: A amostra Ă© constituĂda por 85 colaboradores e o instrumento de recolha de dados agrega questões de caracterização sociodemográfica e profissional e duas escalas, já validadas para avaliar: i) o grau de satisfação nas várias dimensões do trabalho (elaborada pelo Instituto da Segurança Social, 2007) e ii) o estilo de liderança dos diretores tĂ©cnicos (elaborada por Melo, 2004), segundo a perspetiva dos colaboradores. Resultados: Os resultados obtidos indicam que o estilo de liderança relacional Ă© o que promove maior satisfação e o estilo de liderança voltado para a tarefa menor satisfação. Conclusões: O estudo das hipĂłteses permitiu observar que as variáveis: gĂ©nero, tipo de contrato, tempo de serviço e habilitações literárias interferem na satisfação dos colaboradores com o trabalho.info:eu-repo/semantics/publishedVersio
Ambivalence and Phantasm in the Portuguese Colonial Discursive Production on Indians (Mozambique)
Based on a number of Portuguese colonial discourses on the subject of Indian residents of Mozambique and a collection of discourses produced by the leaders of Indian communities upon their colonial “hosts”, we will attempt to show how the absence of a joint encompassing representation of colonial society on the part of many Portuguese whites made the basis of their power seem uncertain and vulnerable. This uncertainty – worsened by a structural doubt regarding the value of the Indian way of thinking and acting – stimulated a process of paranoidization. Especially in the final years of Salazar’s regime, this discursive denial of reciprocity competed with an alternative model of representation of colonial society, characterised by a repetitive use of micro-familial positions in the conceptualization of Imperial encounters. However, this model cannot be discounted as an autistic production of colonial power, mirrored by certain social groups, namely, the leaders of the main Indian communities. We will accordingly explore the hypothesis of a co-authorship, both in the gradual recognition of mutual relations between the Portuguese and Indians, and in the emergence of the family-based model of colonial relations itself.Ă€ partir d’un certain nombre de discours coloniaux portugais relatifs aux indiens habitant au Mozambique, et d’un recueil de discours par les chefs des communautĂ©s indiennes et concernant leurs « hĂ´tes » coloniaux, l’article s’efforce de montrer comment l’absence d’une reprĂ©sentation globale commune de la sociĂ©tĂ© coloniale parmi de nombreux Portugais blancs a fait paraĂ®tre incertaine et vulnĂ©rable la base de leur pouvoir. Cette incertitude –aggravĂ©e par un doute structurel concernant la valeur du mode de pensĂ©e et d’action indien – a favorisĂ© un processus de paranoĂŻa. En particulier au cours des dernières annĂ©es du rĂ©gime de Salazar, cette nĂ©gation discursive de la rĂ©ciprocitĂ© s’est trouvĂ©e en concurrence avec un autre modèle de reprĂ©sentation de la sociĂ©tĂ© coloniale, caractĂ©risĂ© par une utilisation rĂ©pĂ©tĂ©e des positions microfamiliales dans la conceptualisation des rencontres impĂ©riales. Toutefois, ce modèle ne peut pas ĂŞtre rejetĂ© comme une production autiste du pouvoir colonial, reflĂ©tĂ©e par certains groupes sociaux, et spĂ©cifiquement les chefs des principales communautĂ©s indiennes. L’article explore donc l’hypothèse d’une origine commune, Ă la fois dans la reconnaissance progressive des relations rĂ©ciproques entre Portugais et Indiens et dans l’émergence du modèle mĂŞme des relations coloniales fondĂ© sur la famille.Tendo por base um certo nĂşmero de discursos coloniais portugueses sobre o tema dos habitantes indianos de Moçambique e uma colectânea de discursos apresentados pelos lĂderes das comunidades indianas relativamente aos seus « anfitriões »coloniais, o artigo tenta mostrar como a ausĂŞncia de uma representação global comum da sociedade colonial entre muitos portugueses brancos fez com que a base do seu poder parecesse incerta e vulnerável. Esta incerteza – agravada por uma dĂşvida estrutural relativa ao valor da maneira indiana de pensar e de agir – estimulou um processo de paranĂłia. Especialmente no final do regime salazarista, esta negação discursiva da reciprocidade esteve em concorrĂŞncia com um modelo alternativo de representação da sociedade colonial, caracterizada pela utilização repetitiva de posições micro-familiares na conceptualização dos encontros imperiais. No entanto, este modelo nĂŁo pode ser rejeitado como uma produção autista do poder colonial, reflectido por certos grupos sociais, nomeadamente os lĂderes das principais comunidades indianas. O artigo, por conseguinte, explora a hipĂłtese de uma origem comum, ao mesmo tempo no reconhecimento gradual das relações mĂştuas entre os portugueses e os indianos, e na emergĂŞncia de um modelo de relações coloniais ele prĂłprio baseado na famĂlia
Práticas de literacia na aprendizagem de matemática: a escrita de géneros textuais na promoção da autonomia de alunos e de professores do 1.º Ciclo do Ensino Básico
Este trabalho é financiado por Fundos FEDER através do Programa Operacional Factores de Competitividade –
COMPETE e por Fundos Nacionais através da FCT–Fundação para a Ciência e a Tecnologia no âmbito do projeto
FCOMP-01-0124-FEDER-041405 (EXPL/MHC-CED/0645/2013). 1 Este texto foi escrito no âmbito de uma licença sabática da autora, subvencionada pela FCT
(SFRH/BSAB/1440/2014).info:eu-repo/semantics/publishedVersio
CERFI: From the Hospital to the City
The importance of architecture to clinical and psychiatric care is widely asserted. Foucault's writings have demonstrated how architecture has both mirrored and influenced conceptions of mental illness through history. However, this is different from considering space itself an intrinsic factor of the clinical process.
This paper will explore the relationship between the clinical and the spatial trough three instances. It will start at Saint-Alban’s hospital where Tosquelles and Bonnafé laid the ground of the French institutional psychotherapy movement. Here the concern with space first emerges as an economic and political issue. To avoid famine and extinction bars and windows were removed and the doors opened. This allowed establishing a support network with family and village farmers.
The paper then moves to Jean Oury and La Borde clinic. It will explore the theorization of'architectonic relations', 'atmosphere' and 'patoplasty' as the affirmation of space as a non-negligible therapeutic vector. Spatial protocols such the freedom to walk wherever patients wished and the rotation of spatial settings associated with medical functions will be analyzed at this light.
Finally, the paper will focus the work of Guattari. It will examine the link between affirming the importance of spatial factors in the production of an institutional collective (as seen in La Borde) with Guattari's interest in urbanism and his work at CERFI.
Ultimately, the paper aims to show the central importance of space to the clinical and argue how this relation ought to be considered as a question of a wider social and political relevance
A lĂngua portuguesa na formação inicial de educadores de infância e professores do 1Âş ciclo do ensino básico: formação actual e áreas de investigação em perspectiva
Em primeiro lugar, caracteriza-se a formação que Ă© actualmente realizada na área curricular da LĂngua Portuguesa no Departamento de CiĂŞncias Integradas e LĂngua Materna do Instituto de Estudos da Criança, que forma Educadores de Infância e Professores do 1Âş Ciclo. Reflecte-se ainda sobre a(s) finalidade(s) que norteiam este trabalho, descrevendo-se alguns exemplos ilustrativos do tipo de trabalho produzido pelos alunos no âmbito da formação recebida. Por fim, algumas das linhas de trabalho futuro que presentemente se perspectivam para o domĂnio da LĂngua Portuguesa sĂŁo apresentadas e sumariamente discutidas
Sobre a definição das metas de aprendizagem para a educação prĂ©-escolar em Portugal : reflexĂŁo centrada nas aprendizagens de caráter linguĂstico
Neste texto, discute-se a recente definição das metas de aprendizagem para a educação pré-escolar em Portugal,
avaliando em particular o teor das metas de aprendizagem de caráter linguĂstico. O principal argumento que se
defende Ă© o de que assim se inicia uma ĂŞnfase curricular na educação prĂ©-escolar - que se traduz, no domĂnio
especĂfico da linguagem oral e abordagem Ă escrita, na definição de um conjunto de metas conceptualmente
frágeis, restritivas, em diversos casos, local e globalmente incoerentes com outros documentos que regulam
este nĂvel educativo.The recent definition of learning standards for pre-school education in Portugal is discussed in this essay, which
evaluates in particular the contents of the learning standards for language education. The main argument is that
this definition introduces a curricular emphasis to pre-school education, representing, for language and literacy
education, the definition of a set of goals that are conceptually fragile, restricted and, in several cases, locally and
globally incoherent with other documents that regulate this educational level
Writing and the situated construction of teachers’ cognition : portfolios as complex performative spaces
With this paper, I aim to contribute to the understanding of the teaching portfolio as a genre. I analyse the linguistic construction and performative nature of the reflective voice in the portfolio of one pre-service teacher. The results reveal her voice to have been constructed upon the convergence of other voices and to perform diverse reflective actions. The portfolio is conceptualised as a complex performative space that is used to construct a complex thought about practice and, accordingly, verbally act upon the development of the teacher's reflective cognition. I finally equate some research questions that are set by this conceptualisation.This research was financially supported by national funds provided by FCT (Fundação para a Ciência
e a Tecnologia) under the project PEst-OE/CED/UI1661/2014 through CIEd (Centro de Estudos
em Educaçã) at the Universidade do Minho, Portugal
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