The Entropy of Laughter: Discriminative Power of Laughter’s Entropy in the Diagnosis of Depression

Laughter is increasingly present in biomedical literature, both in analytical neurological aspects and in applied therapeutic fields. The present paper, bridging between the analytical and the applied, explores the potential of a relevant variable of laughter’s acoustic signature—entropy—in the detection of a widespread mental disorder, depression, as well as in gauging the severity of its diagnostic. In laughter, the Shannon–Wiener entropy of the distribution of sound frequencies, which is one of the key features distinguishing its acoustic signal from the utterances of spoken language, has not been a specific focus of research yet, although the studies of human language and of animal communication have pointed out that entropy is a very important factor regarding the vocal/acoustic expression of emotions. As the experimental survey of laughter in depression herein undertaken shows, it was possible to discriminate between patients and controls with an 82.1% accuracy just by using laughter’s entropy and by applying the decision tree procedure. These experimental results, discussed in the light of the current research on laughter, point to the relevance of entropy in the spontaneous bona fide extroversion of mental states toward other individuals, as the signal of laughter seems to imply. This is in line with recent theoretical approaches that rely on the optimization of a neuro-informational free energy (and associated entropy) as the main “stuff” of brain processing

Clonal chromosomal mosaicism and loss of chromosome Y in elderly men increase vulnerability for SARS-CoV-2

The pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2, COVID-19) had an estimated overall case fatality ratio of 1.38% (pre-vaccination), being 53% higher in males and increasing exponentially with age. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, we found 133 cases (1.42%) with detectable clonal mosaicism for chromosome alterations (mCA) and 226 males (5.08%) with acquired loss of chromosome Y (LOY). Individuals with clonal mosaic events (mCA and/or LOY) showed a 54% increase in the risk of COVID-19 lethality. LOY is associated with transcriptomic biomarkers of immune dysfunction, pro-coagulation activity and cardiovascular risk. Interferon-induced genes involved in the initial immune response to SARS-CoV-2 are also down-regulated in LOY. Thus, mCA and LOY underlie at least part of the sex-biased severity and mortality of COVID-19 in aging patients. Given its potential therapeutic and prognostic relevance, evaluation of clonal mosaicism should be implemented as biomarker of COVID-19 severity in elderly people. Among 9578 individuals diagnosed with COVID-19 in the SCOURGE study, individuals with clonal mosaic events (clonal mosaicism for chromosome alterations and/or loss of chromosome Y) showed an increased risk of COVID-19 lethality

Cabbage and fermented vegetables : From death rate heterogeneity in countries to candidates for mitigation strategies of severe COVID-19

Large differences in COVID-19 death rates exist between countries and between regions of the same country. Some very low death rate countries such as Eastern Asia, Central Europe, or the Balkans have a common feature of eating large quantities of fermented foods. Although biases exist when examining ecological studies, fermented vegetables or cabbage have been associated with low death rates in European countries. SARS-CoV-2 binds to its receptor, the angiotensin-converting enzyme 2 (ACE2). As a result of SARS-CoV-2 binding, ACE2 downregulation enhances the angiotensin II receptor type 1 (AT(1)R) axis associated with oxidative stress. This leads to insulin resistance as well as lung and endothelial damage, two severe outcomes of COVID-19. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is the most potent antioxidant in humans and can block in particular the AT(1)R axis. Cabbage contains precursors of sulforaphane, the most active natural activator of Nrf2. Fermented vegetables contain many lactobacilli, which are also potent Nrf2 activators. Three examples are: kimchi in Korea, westernized foods, and the slum paradox. It is proposed that fermented cabbage is a proof-of-concept of dietary manipulations that may enhance Nrf2-associated antioxidant effects, helpful in mitigating COVID-19 severity.Peer reviewe

Nrf2-interacting nutrients and COVID-19 : time for research to develop adaptation strategies

There are large between- and within-country variations in COVID-19 death rates. Some very low death rate settings such as Eastern Asia, Central Europe, the Balkans and Africa have a common feature of eating large quantities of fermented foods whose intake is associated with the activation of the Nrf2 (Nuclear factor (erythroid-derived 2)-like 2) anti-oxidant transcription factor. There are many Nrf2-interacting nutrients (berberine, curcumin, epigallocatechin gallate, genistein, quercetin, resveratrol, sulforaphane) that all act similarly to reduce insulin resistance, endothelial damage, lung injury and cytokine storm. They also act on the same mechanisms (mTOR: Mammalian target of rapamycin, PPAR gamma:Peroxisome proliferator-activated receptor, NF kappa B: Nuclear factor kappa B, ERK: Extracellular signal-regulated kinases and eIF2 alpha:Elongation initiation factor 2 alpha). They may as a result be important in mitigating the severity of COVID-19, acting through the endoplasmic reticulum stress or ACE-Angiotensin-II-AT(1)R axis (AT(1)R) pathway. Many Nrf2-interacting nutrients are also interacting with TRPA1 and/or TRPV1. Interestingly, geographical areas with very low COVID-19 mortality are those with the lowest prevalence of obesity (Sub-Saharan Africa and Asia). It is tempting to propose that Nrf2-interacting foods and nutrients can re-balance insulin resistance and have a significant effect on COVID-19 severity. It is therefore possible that the intake of these foods may restore an optimal natural balance for the Nrf2 pathway and may be of interest in the mitigation of COVID-19 severity

Tamaño muestral mínimo y contraste de dos proporciones binomiales.

In this paper, we design an algorithm to calculate the minimum sample size for the two-parameter binomial test. We test H0: p1=p2=p0 against Ha: p1=p0−Δ and p2=p0+Δ, 0<Δ<p0≤1/2, with level of significance (type I error) below α and power function above 1−β (type II error below β). We supply tables that define the decision function for testing the two hypotheses

Presumptive tests: A substitute for Benzidine in blood samples recognition

The nature of the sample in a forensic case is one of the most important factors, since it determines the posterior analysis, helping to define or discard its identity (like blood versus semen). A presumptive test is a qualitative analysis that allows to identify, or confirm, the presence of a substance in a sample. These determinations usually occur, after a chemical reaction, and a specific colour is produced. A false positive is another substance reacting the same way, producing the expected result. The aim of this work was to evaluate the most effective presumptive test (with fewer false-positives) when analysing products that could look and behave like blood during a forensic screening assay. Eight different products were tested, like Betadine®, and four reagents were considered: Tetramethylbenzidine, O-toluidine, Leuchomalachite green and BlueStar® Forensic (BlueStar). Each product was tested with the reagents five times − mixed with human blood (3:1), with three different animal blood (3:1), and then unmixed. Our results indicated that Leuchomalachite green is the most suitable presumptive test, since it was the reagent with less false positives.This work was supported by G/6401400/8000 research project (Santander-Universidad Complutense de Madrid, Spain) for C.Gomes.Peer reviewe

Paleozoic gondwanan structure along the maule river valley between toconey and constitución (35.4°S, chilean pacific coast)

During the late Carboniferous–middle Permian, the basement of the Chilean Coastal Cordillera was deformed by the Gondwanan Orogeny, developed by the subduction of the Paleo-Pacific plate under Gondwana from the early Carboniferous. In the Maule sector (35.4°S), the following metamorphic rock units are distinguished: (i) Western Series, formed by metapelites, metabasites, metacherts, metaconglomerates, and marbles, mixed in a subduction channel, with probably ages between the Cambrian and the Carboniferous; (ii) Eastern Series, consisting of metasediments of a fore-arc basin, Carboniferous in age. In the Eastern Series two units are recognized in terms of lithology and deformation style: the Eastern Pelitic Series and the Eastern Sandy Series. Three Gondwanan deformation episodes have been distinguished, affecting both series unequaly. In the Western Series, D1 structures consist of a foliation (S1) developed under high-pressure and low-temperature conditions, while in the Eastern Series it comprise tight east-verging folds, with the associated S1 developed under low PT metamorphic conditions (greenschist facies). D2 structures are only present in the Western Series and Eastern Pelitic Series and represent the exhumation of the accretionary prism and its emplacement to the east over the fore-arc basin, with the development of east-verging recumbent folds and a thick shear-zone. Difficulties in the propagation of the deformation by the presence of the Coastal Batholith to the east led to the development of the D3 deformation event, only well developed in the Eastern Sandy Series, and represented by the Los Tablones Back-thrust and associated folds, as well as N–S open right folds formed under low PT metamorphic conditions.Peer reviewe

Real‐life report of allergen immunotherapy management during the COVID‐19 outbreak in France and Spain

International audienceNo abstract availabl

Cabbage and fermented vegetables: From death rate heterogeneity in countries to candidates for mitigation strategies of severe COVID-19

Large differences in COVID-19 death rates exist between countries and between regions of the same country. Some very low death rate countries such as Eastern Asia, Central Europe, or the Balkans have a common feature of eating large quantities of fermented foods. Although biases exist when examining ecological studies, fermented vegetables or cabbage have been associated with low death rates in European countries. SARS-CoV-2 binds to its receptor, the angiotensin-converting enzyme 2 (ACE2). As a result of SARS-CoV-2 binding, ACE2 downregulation enhances the angiotensin II receptor type 1 (AT1R) axis associated with oxidative stress. This leads to insulin resistance as well as lung and endothelial damage, two severe outcomes of COVID-19. The nuclear factor (erythroid-derived 2)-like 2 (Nrf2) is the most potent antioxidant in humans and can block in particular the AT1R axis. Cabbage contains precursors of sulforaphane, the most active natural activator of Nrf2. Fermented vegetables contain many lactobacilli, which are also potent Nrf2 activators. Three examples are: kimchi in Korea, westernized foods, and the slum paradox. It is proposed that fermented cabbage is a proof-of-concept of dietary manipulations that may enhance Nrf2-associated antioxidant effects, helpful in mitigating COVID-19 severity.</p