Correlation of the Thanetian-Ilerdian turnover of larger foraminifera and the Initial Eocene thermal maximum (IETM) confirming evidence from the Campo area (Pyrenees, Spain)

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FIELD TRIP TO THE YPRESIAN/LUTETIAN BOUNDARY AT THE GORRONDATXE BEACH SECTION (BASQUE COUNTRY, W PYRENEES)

One of the Paleogene Stage boundaries still needing official definition is the Ypresian/Lutetain (Early- Middle Eocene) boundary. With the aim of contributing to attain this definition, a high-resolution multidisciplinary study, including physical stratigraphy (lithostratigraphy, sequence stratigraphy and magnetostratigraphy) and biostratigraphy (calcareous nannofossil, planktic foraminifer and larger foraminifer), has been carried out over the 700 m thick uppermost Ypresian – lower Lutetian Gorrondatxe section. The results show that the different events traditionally used to place the Ypresian/Lutetian boundary, hitherto thought to be simultaneous (i.e., the planktic foraminifer P9 (=E7) / P10 (=E8) Zone boundary; the calcareous nannofossil CP12a / CP12b Subzone boundary; the larger foraminifer SBZ12 / SBZ13 Zone boundary; and the boundary between magnetic polarity chrons C22n and C21r), actually occur at very different levels. Therefore, before considering any section to place the Ypresian/Lutetian boundary stratotype, the criterion to precisely define this boundary should be selected. To this end, the succession of events pinpointed in the Ypresian/Lutetian boundary interval of the Gorrondatxe beach section might prove a useful database. The Gorrondatxe section fulfils most of the requirements demanded of a prospective stratotype section. In addition, the great sedimentary thickness, which implies a very high deep-marine sedimentation rate, provides the Gorrondatxe section an additional value, as it offers the opportunity to chronologically order successive biomagnetostratigraphic events more precisely than elsewhere. Therefore, we consider that, once the criterion to define the Ypresian/Lutetian boundary is selected, the Gorrondatxe beach section should be deemed a firm candidate to place the Global Stratotype Section and Point of the base of the Lutetian Stage

Cyclostratigraphy of the Early/Middle Eocene transition: a Pyrenean perspective

An integrated bio-, magneto- and cyclostratigraphic study of the Ypresian/Lutetian (Early/Middle Eocene) transition along the Pyrenean Otsakar section (Payros et al., 2011) resulted in the identification of the C22n/C21r chron boundary and of the calcareous nannofossil CP12a/b zonal boundary; the latter is the main correlation criterion of the Lutetian Global Stratotype Section and Point (GSSP) recently defined at Gorrondatxe (Basque Country). By counting precession-related mudstone-marl couplets of 21 ka, the time lapse between both events was calculated to be of 819 ka. This suggests that the age of the CP12a/b boundary, and hence that of the Early/Middle Eocene boundary, is 47.76 Ma, 250 ka younger than previously thought. This age agrees with, and is supported by, estimates from Gorrondatxe based on the time lapse between the Lutetian GSSP and the C21r/C21n boundary. The duration of Chron C21r is estimated at 1.326 Ma. Given that the base of the Eocene is dated at 55.8 Ma, the duration of the Early Eocene is of 8 Ma, 0.8 Ma longer than in current time scales. The Otsakar results further show that the bases of planktic foraminiferal zones E8 and P10 are younger than the CP12a/b boundary. The first occurrence of Turborotalia frontosa, being approximately 550 ka older that the CP12a/b boundary, is the planktic foraminiferal event that lies closest to the Early/Middle Eocene boundary. The larger foraminiferal SBZ12/13 boundary is located close to the CP12a/b boundary and correlates with Chron C21r, not with the C22n/C21r boundary

Correlation of the Thanetian-Ilerdian turnover of larger foraminifera and the Paleocene-Eocene thermal maximum: confirming evidence from the Campo area (Pyrenees, Spain)

It has long been known that a major larger foraminifera turnover (LFT) occurred at the boundary between the Thanetian and Ilerdian stages, but its possible correlation with the Paleocene-Eocene thermal maximum (PETM) was unsuspected until the work of Baceta (1996), and has been controversial ever since. After summarizing the history of this controversy, we present information from three new sections that conclusively resolve the issue, all of them placed less than 2 km to the east of the classical Campo section in the southern Pyrenees. In these three sections, an up to 7 meter-thick intercalation of continental deposits rich in pedogenic carbonate nodules is sandwiched between uppermost Thanetian and lowermost Ilerdian shallow marine carbonates. The d13C composition of 42 pedogenic nodules collected from two of these sections (San Martín and La Cinglera) ranges between –11.4 and -14.3‰ and averages –12.9‰, values that conclusively represent the PETM and for the first time are recorded in sections where the LFT is clearly represented. Further, a high-resolution lithological correlation between Campo and the three new sections across the P-E interval unquestionably demonstrates that the lowermost marine beds with autochthonous specimens of Alveolina vredenburgi (a tell-tale of the LFT) are laterally interfingered –and are therefore coeval- with the nodule-bearing PETM continental deposits. On the basis of the new evidence, the temporal coincidence of the PETM and the LFT can no longer be doubted

Redefinition of the Ilerdian Stage (early Eocene)

The Ilerdian Stage was created by Hottinger and Schaub in 1960 to accommodate a significant phase in the evolution of larger foraminifera not recorded in the northern European basins, and has since been adopted by most researchers working on shallow marine early Paleogene deposits of the Tethys domain. One of the defining criteria of the stage is a major turnover of larger foraminifera, marked by the FO’s of Alveolina vredenburgi (formerly A. cucumiformis) and Nummulites fraasi. There is now conclusive evidence that this turnover was coeval with the onset of the Carbon Isotope Excursion (CIE) and, consequently, with the Paleocene-Eocene (P-E) boundary, a temporal correspondence that reinforces the usefulness of the Ilerdian as a chronostratigraphic subdivision of the early Eocene in a regional context. However, in addition to the paleontological criteria, the definition of the Ilerdian was also based on the designation of two reference sections in the southern Pyrenees: Tremp (stratotype) and Campo (parastratotype). In both sections, the base of the stage was placed at the lowest marine bed containing A. vredenburgi specimens. Using the CIE as a correlation tool we demonstrate that these two marine beds occur at different chronological levels, being older in Campo than in Tremp. Further, we show that both beds are in turn younger than the lowest strata with Ilerdian larger foraminifera at the deep-water Ermua section in the Basque Basin (western Pyrenees). Since the age of stage boundaries must be the same everywhere, the choice of these stratotype sections was misleading, since in practice it resulted in the Ilerdian being used as a facies term rather than as a chronostratigraphic unit. To eliminate that conflict, and yet be respectful with established tradition, we propose to redefine the Ilerdian Stage following a procedure similar to the one used by the International Commission on Stratigraphy to establish global chronostratigraphic standards, namely: by using a “silver spike” to be placed in the Tremp section at the base of the Claret Conglomerate, a widespread lithological unit that in the Tremp Graus Basin coincides with the onset of the CIE. The redefined regional Ilerdian Stage becomes thus directly correlatable to the lower part of the global Ypresian Stage, as currently defined by the International Commission on Stratigraphy

New fossils of Sirenia from the Middle Eocene of Navarre (Western Pyrenees) : the oldest West European sea cow record

Postcranial remains of Sirenia from the early Middle Eocene (late Lutetian) Urbasa-Andia Formation of Navarre (Western Pyrenees) are described. The material consists of two partial atlas vertebrae, one humerus and several dorsal ribs (from Arrasate, Urbasa plateau), and partial dorsal ribs (from Lezaun, Andia plateau). The morphology of the fossils is consistent with referral to Dugongidae, the only sirenian clade known so far in the Middle Eocene of Europe. Moreover, the histological study of the ribs shows that the pachyosteosclerosis of extant Sirenia was definitively present by the early Middle Eocene. The oldest sirenian remains reported to date in the Pyrenean Realm were assigned to the Biarritzian, a regional stage that is currently ascribed either to the middle or to the lower-middle Bartonian. Therefore, the sirenian remains of Lezaun, reliably dated as late Lutetian (SBZ16 zone) in age, are definitively the earliest sirenian fossils known in Western Europe and are among the oldest sea cow records of Europe

The Global Stratotype Section and Point (GSSP) for the base of the Lutetian Stage at the Gorrondatxe section, Spain

The GSSP for the base of the Lutetian Stage (early/ middle Eocene boundary) is defined at 167.85 metres in the Gorrondatxe sea-cliff section (NW of Bilbao city, Basque Country, northern Spain; 43º22'46.47" N, 3º 00' 51.61" W). This dark marly level coincides with the lowest occurrence of the calcareous nannofossil Blackites inflatus (CP12a/b boundary), is in the middle of polarity Chron C21r, and has been interpreted as the maximumflooding surface of a depositional sequence that may be global in extent. The GSSP age is approximately 800 kyr (39 precession cycles) younger than the beginning of polarity Chron C21r, or ~47.8 Ma in the GTS04 time scale. The proposal was approved by the International Subcommission on Paleogene Stratigraphy in February 2010, approved by the International Commission of Stratigraphy in January 2011, and ratified by the International Union of Geological Sciences in April 2011.Published86-1082.2. Laboratorio di paleomagnetismoJCR Journalrestricte

Macrocyclic colibactin induces DNA double-strand breaks via copper-mediated oxidative cleavage.

Colibactin is an assumed human gut bacterial genotoxin, whose biosynthesis is linked to the clb genomic island that has a widespread distribution in pathogenic and commensal human enterobacteria. Colibactin-producing gut microbes promote colon tumour formation and enhance the progression of colorectal cancer via cellular senescence and death induced by DNA double-strand breaks (DSBs); however, the chemical basis that contributes to the pathogenesis at the molecular level has not been fully characterized. Here, we report the discovery of colibactin-645, a macrocyclic colibactin metabolite that recapitulates the previously assumed genotoxicity and cytotoxicity. Colibactin-645 shows strong DNA DSB activity in vitro and in human cell cultures via a unique copper-mediated oxidative mechanism. We also delineate a complete biosynthetic model for colibactin-645, which highlights a unique fate of the aminomalonate-building monomer in forming the C-terminal 5-hydroxy-4-oxazolecarboxylic acid moiety through the activities of both the polyketide synthase ClbO and the amidase ClbL. This work thus provides a molecular basis for colibactin's DNA DSB activity and facilitates further mechanistic study of colibactin-related colorectal cancer incidence and prevention

Buscando a C19n: el potencial de la Formación Mendiorotz (cuenca de Jaca-Pamplona, Pirineos occidentales) para el GSSP del Bartoniense

El cron C19n es el principal marcador del límite Luteciense/Bartoniense, cuyo GSSP está todavía pendiente de ser determi- nado debido a la ausencia de secciones idóneas a nivel mundial. Aunque el Pirineo occidental alberga numerosas cuencas sedimentarias con formaciones marinas de edad Luteciense y Bartoniense, intentos anteriores de encontrar secciones poten- cialmente interesantes en dicha zona no han dado resultados satisfactorios (Payros et al., 2014). En este resumen se presenta el trabajo de campo que se ha realizado en la parte occidental de la cuenca de Jaca-Pamplona con el objetivo de identificar series estratigráficas susceptibles de ser datadas mediante magnetobioestratigráfía y posicionar el límite Luteciense/Bar- toniense. Fruto de este trabajo se ha explorado el sinclinal de Izaga, en cuya parte oriental afloran series de la Formación Mendiorotz, de edad Luteciense superior/Bartoniense inferior (Payros et al., 1999), caracterizadas por una sedimentación pelágica dominante y una exposición y continuidad estratigráfica idóneas para encontrar el marcador del límite, el cron C19n. Chron C19n is the main marker of the Lutetian/Bartonian boundary, whose GSSP is still to be defined due to the lack of suitable sections worldwide. Although the Western Pyrenees host a number of sedimentary basins with marine formations of Lutetian and Bartonian age, previous attempts of finding prospective sections in the area have resulted in an unsuccess- ful outcome (Payros et al., 2014). Here we present the results of a fieldwork study conducted in the western sector of the Jaca-Pamplona basin with the aim of identifying sedimentary successions where the Lutetian/Bartonian boundary can be found through magnetobiostratigraphic dating. As a result of this search, we have focused our attention on the eastern sector of the Izaga syncline, where dominantly pelagic sediments of the Mendiorotz Formation, upper Lutetian/lower Bartonian in age (Payros et al., 1999), crop out with enough exposure and stratigraphic continuity to allow identification of Chron C19n

The Global Stratotype Sections and Points for the bases of the Selandian (Middle Paleocene) and Thanetian (Upper Paleocene) stages at Zumaia, Spain

The global stratotype sections and points for the bases of the Selandian (Middle Paleocene) and Thanetian (Upper Paleocene) stages have been defined in the coastal cliff along the Itzurun Beach at the town of Zumaia in the Basque Country, northern Spain. In the hemipelagic section exposed at Zumaia the base of the Selandian Stage has been placed at the base of the Itzurun Formation, ca. 49 m above the Cretaceous/ Paleogene boundary. At the base of the Selandian, marls replace the succession of Danian red limestone and limestone-marl couplets. The best marine, global correlation criterion for the basal Selandian is the second radiation of the important calcareous nannofossil group, the fasciculiths. Species such as Fasciculithus ulii, F. billii, F. janii, F. involutus, F.pileatus and F. tympaniformis have their first appearance in the interval from a few decimetres below up to 1.1 m above the base of the Selandian. The marker species for nannofossil Zone NP5, F. tympaniformis, first occurs 1.1 m above the base. Excellent cyclostratigraphy and magnetostratigraphy in the section creates further correlation potential, with the base of the Selandian occuring 30 precession cycles (630 kyr) above the top of magnetochron C27n. Profound changes in sedimentology related to a major sea-level fall characterize the Danian-Selandian transition in sections along the margins of the North Atlantic. The base of the Thanetian Stage is placed in the same section ca. 78 m above the Cretaceous/Paleogene boundary. It is defined at a level 2.8 m or eight precession cycles above the base of the core of the distinct clay-rich interval associated with the Mid-Paleocene Biotic Event, and it corresponds to the base of magnetochron C26n in the section. The base of the Thanetian is not associated with any significant change in marine micro-fauna or flora. The calcareous nannofossil Zone NP6, marked by the first occurrence of Heliolithus kleinpelli starts ca. 6.5 m below the base of the Thanetian. The definitions of the global stratotype points for the bases of the Selandian and Thanetian stages are in good agreements with the definitions in the historical stratotype sections in Denmark and England, respectively