Baculovirus expression: tackling the complexity challenge

This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.Most essential functions in eukaryotic cells are catalyzed by complex molecular machines built of many subunits. To fully understand their biological function in health and disease, it is imperative to study these machines in their entirety. The provision of many essential multiprotein complexes of higher eukaryotes including humans, can be a considerable challenge, as low abundance and heterogeneity often rule out their extraction from native source material. The baculovirus expression vector system (BEVS), specifically tailored for multiprotein complex production, has proven itself to be uniquely suited for overcoming this impeding bottleneck. Here we highlight recent major achievements in multiprotein complex structure research that were catalyzed by this versatile recombinant complex expression tool

Closing the light sbottom mass window from a compilation of e+e- -> hadron data

The e+e- -> hadron cross section data from PEP, PETRA, TRISTAN, SLC and LEP, at centre-of-mass energies between 20 to 209 GeV, are analysed to search for the production of a pair of light sbottoms decaying hadronically via R-parity-violating couplings. This analysis allows the 95%C.L. exclusion of such a particle if its mass is below 7.5 GeV/c2. The light sbottom mass window is closed.Comment: 19 pages, 8 figures, 10 tables To be published in Physics Letters. This new version has been reshuffled with respect to the original version to include the suggestions from the Referees of Physics Letters. In particular, the e+e- -> hadron data compilation and combination method are now described in detail, to avoid references to unpublished material. A more realistic treatment of systematic uncertainties also improved the limit slightl

Mr. Jonathan P. Berger: Gentle Conflations

Sentimentality is a critical aspect of human existence because it is human-natural, agendered, and provides ground for gentle conflation of the domestic sphere and the roles within it. As an artist, I am able to utilize sentimentality to open possibilities and welcome, instead of molest, viewers into contemplation with the assumed norms of domesticity. With its origins founded in the Age of Enlightenment, sentimentality was a praiseworthy endeavor, one based on intelligence and contemplation. I define sentimentality as the emotional intellect’s way of encoding or decoding the soft emotions surrounding and within objects, people, times or ideas. Soft emotions are those emotions that when positive warm us and when negative nibble away at us. Because of its foundation in our innate emotional intelligence, sentimentality is a human-natural and agendered phenomenon. I posit that sentimentality can be strategically used to induce gentle conflation between world-representations, especially those located within the domestic. Essentially, world- representations are bundles of facts that are true in some world, be it fictional or non-fictional. Because of their quietness, soft emotions are able to linger mysteriously around and between their source world-representations, blurring their distinctions. Within my artistic practice I contemplate concepts of labor, love and the fine line between loneliness and solitude found within the domestic sphere by utilizing sentimentality as a tool of gentle conflation

Concert recording 2016-01-21

[Track 01]. Années de Pelèrinage : première année. Pastorale ; Le mal du pays / Franz Liszt -- [Track 02]. Es rauschen die Winde ; Über allen Gipfeln ist Ruh ; Im Rhein im schönen Strome / Franz Liszt -- [Track 03]. Dichterliebe. Im wunderschönen Monat Mai ; Aus meinen Tränen spriessen ; Die Rose, die Lilie, die Taube ; Wenn ich in deine Augen seh\u27 ; Ich will meine Seele tauchen ; Im Rhein, im heiligen Strome / Robert Schumann -- [Track 04]. Novelette op. 21, no. 8 / Robert Schumann -- [Track 05]. Banalités. Chanson d\u27Orkenise ; Hôtel ; Fagnes de Wallonie ; Voyage à Paris / Francis Poulenc -- [Track 06]. Quatre poèmes de Guillaume Apollinaire. L\u27Anguille ; Carte postale ; Avant le cinéma ; 1904 / Francis Poulenc -- [Track 07]. The show is on. By Strauss / George Gershwin

The Growing Complexity of Internet Interconnection

End-to-End (E2E) packet delivery in the Internet is achieved through a system of interconnections between heterogeneous entities called Autonomous Systems (ASes). The initial pattern of AS interconnection in the Internet was relatively simple, involving mainly ISPs with a balanced mixture of inbound and outbound traffic. Changing market conditions and industrial organization of the Internet have jointly forced interconnections and associated contracts to become significantly more diverse and complex. The diversity of interconnection contracts is significant because efficient allocation of costs and revenues across the Internet value chain impacts the profitability of the industry. Not surprisingly, the challenges of recovering the fixed and usage-sensitive costs of network transport give rise to more complex settlements mechanisms than the simple bifurcated (transit and peering) model described in many earlier analyses of Internet interconnection (see BESEN et al., 2001; GREENSTEIN, 2005; or LAFFONT et al., 2003). In the following, we provide insight into recent operational developments, explaining why interconnection in the Internet has become more complex, the nature of interconnection bargaining processes, the implications for cost/revenue allocation and hence interconnection incentives, and what this means for public policy. This paper offers an abbreviated version of the original paper (see FARATIN et al., 2007b).internet interconnection, economics, public policy, routing, peering.