Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865)

The Puntius denisonii colloquially and more popularly referred to as Miss Kerala is a subtropical fish belonging to the genus Puntius (Barb) and family Cyprinidae. Two cell lines PDF and PDH were developed from the caudal fin and heart of P. denisonii, respectively. The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines. The high growth potential of the cell lines was reflected from the cell doubling time of 28 and 30 h of PDF and PDH cell lines, respectively. The viability of the PDF and PDH cell lines was 70% and 76%, respectively, after 4 mo of storage in liquid nitrogen (−196°C). The origin of the cell lines was confirmed by the amplification of 653 bp fragments of cytochrome oxidase subunit I of mitochondrial DNA genes

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Not AvailableThe present study describes the length–weight relationships (LWRs) of two important ornamental hill stream fishes, Barilius bendelisis and Barilius vagra (Hamilton), of northern India. A total of 630 specimens were collected from January to June 2011 in a spring-fed stream in Garhwal Himalaya, India. Information regarding LWRs of these species was not yet available in FishBase.Not Availabl

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Not AvailableThere is a significant taxonomic ambiguity among snow trout species due to their morphometric similarities. In view of this, a morphometric and molecular study was conducted on five different species of genus Schizothorax that have been reported from Kashmir valley. Morphometric data analyzed using multivariate statistics (Principal component analysis and cluster analysis) indicated the significant grouping of species to individual clusters. Mitochondrial DNA cytochrome oxidase I (COI) gene analysis revealed 0.2%–4.5% genetic divergence among the five species. This study confirms that utility of cytochrome oxidase I in species delineation along with morphometric data. Phylogenetic tree obtained using Neighbor-Joining method revealed that all the five species represented distinct species group. The Schizothorax genus formed two distinct clades; one containing S. niger, S. curvifrons and S. plagiostomus, while other clade containing S. esocinus and S. labiatus. This phlogeny trend was also supported by cluster analysis of morphometric characters. The phylogenetic analysis with other published COI sequences revealed distinct nature of these five species. The study may aid in the taxonomic identification of snow trout species in India. This may further increase the knowledge of the ichthyologists in planning conservation and management strategies for these important fish species along with their natural habitat.Not Availabl

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Not AvailableLength-weight relationships (LWRs) are described for 10 fish species belonging to three families from the Gaula River, a tributary of the Ganga River Basin in India. LWRs for these species were unknown to FishBase, and new maximum lengths are recorded for one species. These results will be useful for fishery research, management and conservation in the least-explored tributary of the Ganga River Basin.Not Availabl

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Not Availablehe golden mahseer, Tor putitora, is the most popular sport fish in most of the large rivers, lakes and reservoirs of India, Pakistan, Bangladesh and Nepal. This is also reported from Sri Lanka, Afghanistan, Myanmar, Turkey and Papua New Guinea. The species is fast being depleted in natural waters due to various anthropogenic factors. Recent success in captive breeding has shown a great potential for its aquaculture in the uplands of India. The mathematical relationship between length and weight of fishes is a practical index suitable for understanding their survival, growth, maturity, reproduction, and general well being (LeCren, 1951). Length–weight studies in fishes are made to estimate population strengths as well as to measure variations in expected weights due to changes in their life cycles (LeCren, 1951; Beverton and Holt, 1959).Not Availabl

Temporal Study of Physicochemical Characteristics and Qualitative Plankton Diversity of a River Kosi, a Tributary of West Ramganga in Reference to the Habitat of Garra gotyla gotyla

The river, Kosi originates from the western slope of the Kaushani range and flows (mountains of Almora, Nainital and Udham Singh Nagar) in the southern direction and joins the river, Western Ramganga at tarai region. Along with the total catchment area of 3,420 sq.km., river Kosi covers a distance of 240 km. The present study determines the habitat at of Garra gotyla, physicochemical parameters of water and plankton diversity of river Kosi from January 2019 to December 2019. During the study, various physicochemical parameters such as air temperature, water temperature, pH, dissolved oxygen, dissolved free carbon dioxide, electrical conductivity, velocity, transparency, total dissolved solids, and total alkalinity were analyzed. The biological study was focused on the qualitative assessment of phytoplankton and zooplankton diversity. A total of 34 species of phytoplankton as well as 13 species of zooplankton were recorded during the present study and among phytoplankton, the dominant group was the members of the class- Bacillariophyceae

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Not AvailableThe induced breeding of Koi Carp ( Cyprinus carpio carpio) was successfully carried out at the Directorate of Coldwater Fisheries Research, Bhimtal, India. Induced breeding and rearing conducted on the fish revealed that the fish can be breed at low temperature using sGnRH analogue and dopamine antagonist (Ovaprim). Spawning was observed eighteen hrs after the injection, fertilizationand hatching and fry survival rate were observed in fishes spawned in April (70%, 75% and 42%, respectively) than fishes spawnedin August (80-85%, 90-95% and 80% respectively).The incubation period was 120 hours and 84 hours at temperature 15-18 15-18o C(April)and 20-26o C (August), respectively. Present study revealed that favourable conditions on hill region for mass seed production of koi carp in the month of April.Not Availabl

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Not AvailableThe Puntius denisonii colloquially and more popularly referred to as Miss Kerala is a subtropical fish belonging to the genus Puntius (Barb) and family Cyprinidae. Two cell lines PDF and PDH were developed from the caudal fin and heart of P. denisonii, respectively. The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines. The high growth potential of the cell lines was reflected from the cell doubling time of 28 and 30 h of PDF and PDH cell lines, respectively. The viability of the PDF and PDH cell lines was 70% and 76%, respectively, after 4 mo of storage in liquid nitrogen (−196°C). The origin of the cell lines was confirmed by the amplification of 653 bp fragments of cytochrome oxidase subunit I of mitochondrial DNA genes.Not Availabl

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Not AvailableThe Puntius denisonii colloquially and more popularly referred to as Miss Kerala is a subtropical fish belonging to the genus Puntius (Barb) and family Cyprinidae. Two cell lines PDF and PDH were developed from the caudal fin and heart of P. denisonii, respectively. The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines. The high growth potential of the cell lines was reflected from the cell doubling time of 28 and 30 h of PDF and PDH cell lines, respectively. The viability of the PDF and PDH cell lines was 70% and 76%, respectively, after 4 mo of storage in liquid nitrogen (−196°C). The origin of the cell lines was confirmed by the amplification of 653 bp fragments of cytochrome oxidase subunit I of mitochondrial DNA genes.Not Availabl

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Not AvailableCyprinus carpio (var. communis) and Carassius carassius (family Cyprinidae) are morphologically well characterized fish species but the ambiguity persists in their identification at very juvenile stage. This ambiguity could be more harmful in case of initial stocking of fries or in processed fish products that lack distinct morphological characters. The present study was conducted to characterize these species using molecular markers which could aid their decisive characterization. The molecular characterization was carried out using COI gene (620 bp) region. The results revealed a very low intra-specific nucleotide sequence divergence within both the species, whereas mean inter-specific nucleotide variation between the species was 0.154 (range 0.133–0.164). The sequences were also compared to the other species of genus Cyprinus and Carassius to depict their relationship with congeneric species as well as validation of molecular characterization. The topology of phylogenetic tree indicates the hetero-specificity of studied species from one another as well as to their congeneric one. Both the species were clustered in individual clades and grouped neighboring to the available reference sequences of the species. On the basis of present study it may concluded that CO1 gene region (620 bp) is suitable for molecular characterization of both the species which will also supplement their morphological characterization. Also the sequences can be utilized as reference in future molecular studies like population genetics and stock structure analysis of both the species.Not Availabl