Antiproliferative Activity And Induction Of Apoptosis In Pc-3 Cells By The Chalcone Cardamonin From Campomanesia Adamantium (myrtaceae) In A Bioactivity-guided Study.

The Myrtaceae family is a common source of medicines used in the treatment of numerous diseases in South America. In Brazil, fruits of the Campomanesia species are widely used to make liqueurs, juices and sweets, whereas leaves are traditionally employed as a medicine for dysentery, stomach problems, diarrhea, cystitis and urethritis. Ethanol extracts of Campomanesia adamantium (Myrtaceae) leaves and fruits were evaluated against prostate cancer cells (PC-3). The compound (2E)-1-(2,4-dihydroxy-6-methoxyphenyl)-3-phenylprop-2-en-1-one, cardamonin) was isolated from ethanol extracts of C. adamantium leaves in a bioactivity-guided study and quantified by UPLC-MS/MS. In vitro studies showed that the isolated chalcone cardamonin inhibited prostate cancer cell proliferation and decreased the expression of NFkB1. Moreover, analysis by flow cytometry showed that this compound induced DNA fragmentation, suggesting an effect on apoptosis induction in the PC-3 cell line.191843-5

Protocolo de ozonioterapia na prevenção da osteonecrose dos maxilares induzida por medicamentos em modelo animal

Reports of osteonecrosis of the jaws are frequent in patients on chronic use of antiresorptives and antiangiogenic medications. Studies have shown that ozone therapy can stimulate cell proliferation and wound healing. The aim of this study was to evaluate the histological areas of extraction in rats previously submitted to Medication-related osteonecrosis of the jaw (MRONJ) under topic aplication of ozonated oil. Twelve (12) Wistar rats were divided into two groups: Negative Control (G1, n=5), these rats received injection of zoledronic acid solution once a week for five weeks and extraction of the first maxillary molar and maintenance of the alveolus with clot; Positive Control (G2, n=7), these rats received injection of zoledronic acid solution once a week for five weeks and extraction of the first upper molar and maintenance of the alveolus with application of ozonated oil for three consecutive days. Extractions were performed on the 7th week after osteonecrosis induction protocol and then area block resection was performed on the 15th week after induction. Histopathological analysis revealed that all of the animals of G1 group presented non-vital areas with empty osteocyte lacunae (indicative of osteonecrosis) associated to a varied amount of bacterial colonies. In G2 group, 57.13% had vital bone, and 14.28% presented bacterial colonies. In 42.85% of the samples from the G2 group there were discrete osteonecrotic areas, however, in 14.28%, no bacterial colonies were found. This study shows positive results of the ozoned oil used to prevent the development of MRONJ in rats and it should be considered for future clinical trials in prevention of MRONJ lesionsSão frequentes os relatos de osteonecrose dos maxilares em pacientes que fazem uso crônico de medicações antirresorptivas e antiangiogênicas. Estudos demonstram que a Ozonioterapia pode estimular a proliferação celular e a cicatrização. O objetivo deste trabalho foi avaliar histologicamente áreas de exodontia em ratos induzidos a Osteonecrose dos Maxilares Induzida por Medicamentos (OMIM) após uso do óleo ozonizado. Doze (12) ratos da raça Wistar albinus foram divididos em 2 grupos, sendo estes: Grupo Controle Negativo (G1, n=5 ratos), onde receberam injeção intravenosa de ácido zoledrônico semanalmente durante cinco semanas; exodontia de primeiro molar superior direito e manutenção do alvéolo com coágulo; Grupo Controle Positivo (G2, n=7 ratos), que receberam injeção de solução ácido zoledrônico semanalmente durante cinco semanas; exodontia de primeiro molar superior direito com aplicação de óleo ozonizado diretamente no alvéolo por três dias consecutivos. As extrações foram realizadas na 7ª semana após o início do protocolo de indução dos grupos. Já as ressecções em bloco das regiões de interesse foram realizadas na 15ª semana após início da indução. A análise histopatológica revelou que no Grupo G1 todas as amostras apresentaram áreas de osso não-vital com lacunas de osteócitos vazias (indicando osteonecrose) associadas a variadas colônias bacterianas; No Grupo G2, 57.13% das amostras não apresentou nenhuma área de osteonecrose, e dentro destes, 14.28% apresentou colônias bacterianas. Em 42.85% das amostras do grupo G2 houve pequenas áreas osteonecróticas, porém, em 14.28%, constatou-se a ausência de colônias. O estudo demonstra resultados positivos quanto ao óleo ozonizado utilizado para prevenir o desenvolvimento de OMIM em ratos, e deve ser considerado em futuros estudos clínicos para a prevenção desta lesã