Nicotiana benthamiana protein, NbPCIP1, interacting with Potato virus X coat protein plays a role as susceptible factor for viral infection

AbstractThe interactions of viral coat protein (CP) and host factors play an important role in viral replication and/or host defense mechanism. In this study, we constructed Nicotiana benthamiana cDNA library to find host factors interacting with Potato virus X (PVX) CP. Using yeast two-hybrid assay, we screened 3.3×106 independent yeast transformants from N. benthamiana cDNA library and identified six positive clones. One positive clone, named PVX CP-interacting protein 1 (NbPCIP1), is a plant-specific protein with homologue in N. tabacum (GenBank accession no. AB04049). We confirmed the PVX CP–NbPCIP1 interaction using yeast-two hybrid assay in yeast, protein–protein binding assay in vitro, and bimolecular fluorescent complementation assay in planta. Quantitative real-time RT-PCR analysis showed that the mRNA level of NbPCIP1 increased in PVX-infected N. benthamiana plants as compared to that of healthy plants. The green fluorescent protein (sGFP)-fused NbPCIP1 (NbPCIP1-sGFP) was localized in ER or ER-associated granular-like structure of cells. When we co-express NbPCIP1-sGFP and red fluorescent protein (RFP)-fused PVX CP (PVX CP-RFP), which were introduced by transiently expressing these proteins in N. benthamiana protoplasts and epidermal cells, however, we observed the co-localization of these proteins in the inclusion body-like complex in areas surrounding nucleus. Transient over-expression and transgene silencing of NbPCIP1 assay analysis indicated that NbPCIP1 plays a critical role in viral replication during PVX infection in host plant

Tangible AR interaction based on fingertip touch using small-sized nonsquare markers

AbstractAlthough big-sized markers are good for accurate marker recognition and tracking, they are easily occluded by other objects and deteriorate natural visualization and level of immersion during user interaction in AR environments. In this paper, we propose an approach to exploiting the use of rectangular markers to support tangible AR interaction based on fingertip touch using small-sized markers. It basically adjusts the length, width, and interior area of rectangular markers to make them more suitably fit to longish objects like fingers. It also utilizes convex polygons to resolve the partial occlusion of a marker and properly enlarges the pattern area of a marker while adjusting its size without deteriorating the quality of marker detection. We obtained encouraging results from users that the approach can provide better natural visualization and higher level of immersion, and be accurate and tangible enough to support a pseudo feeling of touching virtual products with human hands or fingertips during design evaluation of digital handheld products

Environmental Regulations in Private and Mixed Duopolies: Emission Taxes versus Green R&D Subsidies

In the presence of R&D spillovers, we compare environmental regulations between an emission taxes and green R&D subsidies in private and mixed duopoly markets. We show that the green R&D subsidy is better (worse) than the emission tax when the green R&D cost is low (high) irrespective of the R&D spillovers, whereas the existence of a public firm encourages the government to adopt the subsidy policy. We then show that the optimal policy choice depends on the level of the R&D cost and the degree of R&D spillovers. In particular, when the R&D cost is high and the spillover rate is (not) weak, the government should choose the emission tax and (not) privatize the public firm. However, when the R&D cost is low, such a privatization policy is not desirable to society irrespective of the R&D spillovers

Cloning and characterization of RIP1, an effector of Ral

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 1996.Includes bibliographical references (leaves 112-142).by Sang-Ho Park.Ph.D

An Efficient ISAR Imaging Method for Multiple Targets

This paper proposes an efficient method to obtain TSAR images of multiple targets flying in formation. The proposed method improves the coarse alignment and segmentation of the existing method. The improved coarse alignment method models the flight trajectory using a combination of a polynomial and Gaussian basis functions, and the optimum parameter of the trajectory is found using particle swarm optimization. In the improved segmentation, the binary image of the bulk TSAR image that contains all targets is constructed using a two-dimensional constant false alarm detector, then the image closing method is applied to the binary image. Finally, the connected set of binary pixels is used to segment each target from the bulk image. Simulations using three targets composed of point scattering centers and the measured data of the Boeing747 aircraft prove the effectiveness of the proposed method to segment three targets flying in formation.X113Ysciescopu

Running economy - a comprehensive review for passive force generation

Running economy is considered a major determinant of distance running performance. Enhancing the body’s ability for passive force generation could have a positive effect on running economy by minimizing the energy cost required for the propulsion of the body. Thus, the purpose of this comprehensive review was to provide a list of modifiable factors that promote this ability. The interest was focused on lower-limb stiffness, as it is a factor of great influence and at the same time can be modified with training and specific biomechanical adjustments. Although it appears that no clear instructions can be provided to athletes and coaches, it should be noted that careful consideration of the runners’ anthropometric, physiological, and biomechanical characteristics are necessary for optimal performance results

Efficiency Analysis of Major Container Ports in Asia: Using DEA and Shannon’s Entropy

This paper attempts to evaluate performance (i.e. efficiency) of Asias container ports. Measurement of the ports performance is critical to increase the competitiveness of maritime transport, ultimately leading to one nations competitive advantages over other countries. Data Envelopment Analysis (DEA), which is a non-parametric method widely used for assessing efficiency of units which have similar characteristics, was selected to analyse the data. Due to the limitations of the DEA method producing diverse results according to different models, and to the complexities of choosing a specific model among several DEA models, Shannons Entropy was also employed. By including Shannons Entropy, the efficiency results calculated from each model were integrated in order to rank the ports. The results in this study will provide port managers with valuable information in order to understand the current status of Asias container ports in terms of their efficiency

Dynamical mean-field theory of Hubbard-Holstein model at half-filling: Zero temperature metal-insulator and insulator-insulator transitions

We study the Hubbard-Holstein model, which includes both the electron-electron and electron-phonon interactions characterized by $U$ and $g$, respectively, employing the dynamical mean-field theory combined with Wilson's numerical renormalization group technique. A zero temperature phase diagram of metal-insulator and insulator-insulator transitions at half-filling is mapped out which exhibits the interplay between $U$ and $g$. As $U$ ($g$) is increased, a metal to Mott-Hubbard insulator (bipolaron insulator) transition occurs, and the two insulating states are distinct and can not be adiabatically connected. The nature of and transitions between the three states are discussed.Comment: 5 pages, 4 figures. Submitted to Physical Review Letter