Dynamic fluctuations in a Short-Range Spin Glass model

We study the dynamic fluctuations of the soft-spin version of the Edwards-Anderson model in the critical region for $T\rightarrow T_{c}^{+}$. First we solve the infinite-range limit of the model using the random matrix method. We define the static and dynamic 2-point and 4-point correlation functions at the order $O(1/N)$ and we verify that the static limit obtained from the dynamic expressions is correct. In a second part we use the functional integral formalism to define an effective short-range Lagrangian $L$ for the fields $\delta Q^{\alpha\beta}_{i}(t_{1},t_{2})$ up to the cubic order in the series expansion around the dynamic Mean-Field value $\overline{{Q}^{\alpha\beta}}(t_{1},t_{2})$. We find the more general expression for the time depending non-local fluctuations, the propagators $[\langle\delta Q^{\alpha\beta}_{i}(t_{1},t_{2}) \delta Q^{\alpha\beta}_{j}(t_{3},t_{4})\rangle_{\xi}]_{J}$, in the quadratic approximation. Finally we compare the long-range limit of the correlations, derived in this formalism, with the correlations of the infinite-range model studied with the previous approach (random matrices).Comment: 25 pages, LaTeX, 5 figures available upon request. Revised version, to be published on Journal de physiqu

Discovery of a FR0 radio galaxy emitting at γ\gamma-ray energies

We present supporting evidence for the first association of a Fermi source, 3FGLJ1330.0-3818, with the FR0 radio galaxy Tol1326-379. FR0s represent the majority of the local radio loud AGN population but their nature is still unclear. They share the same properties of FRIs from the point of view of the nuclear and host properties, but they show a large deficit of extended radio emission. Here we show that FR0s can emit photons at very high energies. Tol1326-379 has a GeV luminosity of $L_{>1~{\rm GeV}} \sim 2\times10^{42}$ erg s$^{-1}$, typical of FRIs, but with a steeper $\gamma$-ray spectrum ($\Gamma=2.78\pm 0.14$). This could be related to the intrinsic jet properties but also to a different viewing angle.Comment: 7 pages, 5 figures , accepted for publication on MNRA

X-ray study of a sample of FR0 radio galaxies: unveiling the nature of the central engine

FR0s are compact radio sources that represent the bulk of the Radio-Loud (RL) AGN population, but they are still poorly understood. Pilot studies on these sources have been already performed at radio and optical wavelengths: here we present the first X-ray study of a sample of 19 FR0 radio galaxies selected from the SDSS/NVSS/FIRST sample of Best & Heckman (2012), with redshift $\leq$ 0.15, radio size $\leq$ 10 kpc and optically classified as low-excitation galaxies (LEG). The X-ray spectra are modeled with a power-law component absorbed by Galactic column density with, in some cases, a contribution from thermal extended gas. The X-ray photons are likely produced by the jet as attested by the observed correlation between X-ray (2-10 keV) and radio (5 GHz) luminosities, similar to FRIs. The estimated Eddington-scaled luminosities indicate a low accretion rate. Overall, we find that the X-ray properties of FR0s are indistinguishable from those of FRIs, thus adding another similarity between AGN associated with compact and extended radio sources. A comparison between FR0s and low luminosity BL Lacs, rules out important beaming effects in the X-ray emission of the compact radio galaxies. FR0s have different X-ray properties with respect to young radio sources (e.g. GPS/CSS sources), generally characterized by higher X-ray luminosities and more complex spectra. In conclusion, the paucity of extended radio emission in FR0s is probably related to the intrinsic properties of their jets that prevent the formation of extended structures, and/or to intermittent activity of their engines.Comment: Accepted for publication in MNRAS (18 pages, 4 figures

Dynamical fluctuations in an exactly solvable model of spin glasses

In this work we calculate the dynamical fluctuations at O(1/N) in the low temperature phase of the $p=2$ spherical spin glass model. We study the large-times asymptotic regimes and we find, in a short time-differences regime, a fluctuation dissipation relation for the four-point correlation functions. This relation can be extended to the out of equilibrium regimes introducing a function $X_{t}$ which, for large time $t$, we find scales as $t^{-1/2}$ as in the case of the two-point functions.Comment: Latex, 8 page

Macromolecular and Solution Properties of the Recombinant Fusion Protein HUG

The recombinant fusion protein HELP-UnaG (HUG) is a bifunctional product that exhibits human elastin-like polypeptide (HELP)-specific thermal behavior, defined as a reverse phase transition, and UnaG-specific bilirubin-dependent fluorescence emission. HUG provides an interesting model to understand how its two domains influence each other's properties. Turbidimetric, calorimetric, and light scattering measurements were used to determine different parameters for the reverse temperature transition and coacervation behavior. This shows that the UnaG domain has a measurable but limited effect on the thermal properties of HELP. Although the HELP domain decreased the affinity of UnaG for bilirubin, HUG retained the property of displacing bilirubin from bovine serum albumin and thus remains one of the strongest bilirubin-binding proteins known to date. These data demonstrate that HELP can be used to create new bifunctional fusion products that pave the way for expanded technological applications

Materials derived from the human elastin-like polypeptide fusion with an antimicrobial peptide strongly promote cell adhesion

Protein and peptide materials have attracted great interest in recent years, especially for biological applications, in light of their possibility to easily encode bioactivity whilst maintaining cytocompatibility and biodegradability. Heterologous recombinant expression to produce antimicrobial peptides is increasingly considered a convenient alternative for the transition from conventional methods to more sustainable production systems. The human elastin-like polypeptide (HELP) has proven to be a valuable fusion carrier, and due to its cutting-edge properties, biomimetic materials with antimicrobial capacity have been successfully developed. In this work, we have taken advantage of this platform to produce a difficult-to-synthesise sequence as that of the human β-defensin 1 (hBD1), an amphipathic cationic peptide with structural folding constraints relevant to its bioactivity. In the design of the gene, highly specific endoproteinases recognition sites were introduced to release the active forms of hBD1. After the expression and purification of the new fusion construct, its biological activity was evaluated. It was found that both the fusion biopolymer and the released active forms can inhibit the growth of Escherichia coli in redox environments. Remarkably, 2D and 3D materials derived from the biopolymer showed a strong cell adhesion-promoting activity. These results suggest that HELP represents a multitasking platform that not only facilitates the production of bioactive domains and derived materials but could also pave the way for the development of new approaches to study biological interactions at the molecular level

Increased levels of DNA methyltransferases are associated with the tumorigenic capacity of prostate cancer cells

DNA methylation might be the earliest somatic genome changes in prostate cancer that also play an important role in the process of tumor invasion, growth and metastasis. In recent years, several inhibitors of DNA methyltransferases (DNMTis) have been developed and evaluated in pre-clinical models and in clinical trials. While these compounds are effective in the treatment of hematological conditions, clinical trials in solid tumors and in prostate cancer have shown limited or no efficacy. This may be attributed to inappropriate dose regimens leading to toxicity-related adverse events. As with other anti-target compounds, one of the obstacles encountered with DNMTis in prostate cancer could be the inability to select patients for the clinical studies as well as the inability to monitor the efficacy of the drug if not the conclusion of the study. Primary cultures derived from human prostatic tissues harvested from patients with benign prostatic hyperplasia (BPH) and prostate cancer (PCa) as well as neoplastic and non-neoplastic prostate cell lines were tested for DNMT expression/activity and to monitor azacitidine molecular efficacy. We observed that in primary cultures the levels of DNMT activity as well as the protein levels of DNMT1, DNMT3a and DNMT3b were higher in cultures derived from PCa compared to BPH tissue samples and significantly higher in cultures derived from PCa with Gleason scores ≥7 compared to those observed in cultures derived from Gleason scores <7. In addition, DNMT activity as well as DNMT1, DNMT3a and DNMT3b levels were higher in PCa cell lines compared to their non-neoplastic counterparts. Although DNMT activity was higher in high tumorigenic/aggressive PCa cell lines compared to low tumorigenic/aggressive cell lines, only the levels of DNMT3a and DNMT3b were significantly higher in the first group of cells, suggesting that DNMT1 activity is related to the transition to non-neoplastic versus neoplastic phenotype whereas the de novo methylation enzymes were mainly related to progression. Nevertheless, the comparison in the more aggressive PC3 cell derivatives (PC3-LN4 cells) also possessed higher levels of DNMT1 compared to PC3 and PC3M from which these cells were derived. Collectively, our results confirm previous data on the increased methylation in more aggressive tumors supporting the use of DNMTis in advanced prostate cancer. In addition, since glutathione S-transferase-π (GSTP1) was re-expressed or its protein levels were increased after treatment with non-toxic azacitidine doses and since GSTP1 can easily be measured in patient sera, the monitoring of this protein may aide in the evaluation of therapy in future clinical trials

Diatom Polysaccharides: Extracellular Production, Isolation and Molecular Characterization

The extracellular polysaccharide production by marine diatoms is a significant route by which photosynthetically produced organic carbon enters the trophic web and may influence the physical environment in the sea as observed for example when massive aggregation events on basin scale occur. Many papers showed that the aldose signatures of marine DOM obtained from different seawater samples around the world is similar to that determined on cultured phytoplankton DOM and that the carbohydrate production could be very different among the species selected, growth and environmental conditions. These results are very important in order to understand the role of algal exudation in the aggregation processes observed in all of the seas and in general in carbon cycling in the euphotic zone. Many authors showed that cultured diatoms growth in P-limiting condition determines an increase of polysaccharides exudated by different diatoms species both pelagic and benthic