Stellar surface magneto-convection as a source of astrophysical noise II. Center-to-limb parameterisation of absorption line profiles and comparison to observations

Manifestations of stellar activity (such as star-spots, plage/faculae, and convective flows) are well known to induce spectroscopic signals often referred to as astrophysical noise by exoplanet hunters. For example, setting an ultimate goal of detecting true Earth-analogs demands reaching radial velocity (RV) precisions of ~9 cm/s. While this is becoming technically feasible with the latest generation of highly stabilised spectrographs, it is astrophysical noise that sets the true fundamental barrier on attainable RV precisions. In this paper we parameterise the impact of solar surface magneto-convection on absorption line profiles, and extend the analysis from the solar disc centre (Paper I) to the solar limb. Off disc-centre, the plasma flows orthogonal to the granule tops begin to lie along the line-of-sight and those parallel to the granule tops are no longer completely aligned with the observer. Moreover, the granulation is corrugated and the granules can block other granules, as well as the intergranular lane components. Overall, the visible plasma flows and geometry of the corrugated surface significantly impact the resultant line profiles and induce centre-to-limb variations in shape and net position. We detail these herein, and compare to various solar observations. We find our granulation parameterisation can recreate realistic line profiles and induced radial velocity shifts, across the stellar disc, indicative of both those found in computationally heavy radiative 3D magnetohydrodynamical simulations and empirical solar observations.Comment: 17 pages, 14 figures, accepted to Ap

Unsigned magnetic flux as a proxy for radial-velocity variations in Sun-like stars

We estimate disc-averaged RV variations of the Sun over the last magnetic cycle, from the single Fe I line observed by SDO/HMI, using a physical model for rotationally modulated magnetic activity that was previously validated against HARPS-N solar observations. We estimate the disc-averaged, unsigned magnetic flux and show that a simple linear fit to it reduces the RMS of RV variations by 62%, i.e. a factor of 2.6. We additionally apply the FF' method, which predicts RV variations based on a star's photometric variations. At cycle maximum, we find that additional physical processes must be at play beyond suppression of convective blueshift and velocity imablances resulting from brightness inhomogeneities, in agreement with recent studies of solar RV variations. By modelling RV variations over the magnetic cycle using a linear fit to the unsigned magnetic flux, we recover injected planets at an orbital period of about 300 days with RV semi-amplitudes down to 0.3 m/s. To reach semi-amplitudes of 0.1 m/s, we will need to identify and model additional physical phenomena that are not well traced by the unsigned magnetic flux or FF'. The unsigned magnetic flux is an excellent proxy for rotationally modulated, activity-induced RV variations, and could become a key tool in confirming and characterising Earth analogs orbiting Sun-like stars. The present study motivates ongoing and future efforts to develop observation and analysis techniques to measure the unsigned magnetic flux at high precision in slowly rotating, relatively inactive stars like the Sun.Comment: 25 pages, 11 figures, 3 tables, submitted to Ap

The EXPRES Stellar Signals Project II. State of the Field in Disentangling Photospheric Velocities

Measured spectral shifts due to intrinsic stellar variability (e.g., pulsations, granulation) and activity (e.g., spots, plages) are the largest source of error for extreme-precision radial-velocity (EPRV) exoplanet detection. Several methods are designed to disentangle stellar signals from true center-of-mass shifts due to planets. The Extreme-precision Spectrograph (EXPRES) Stellar Signals Project (ESSP) presents a self-consistent comparison of 22 different methods tested on the same extreme-precision spectroscopic data from EXPRES. Methods derived new activity indicators, constructed models for mapping an indicator to the needed radial-velocity (RV) correction, or separated out shape- and shift-driven RV components. Since no ground truth is known when using real data, relative method performance is assessed using the total and nightly scatter of returned RVs and agreement between the results of different methods. Nearly all submitted methods return a lower RV rms than classic linear decorrelation, but no method is yet consistently reducing the RV rms to sub-meter-per-second levels. There is a concerning lack of agreement between the RVs returned by different methods. These results suggest that continued progress in this field necessitates increased interpretability of methods, high-cadence data to capture stellar signals at all timescales, and continued tests like the ESSP using consistent data sets with more advanced metrics for method performance. Future comparisons should make use of various well-characterized data sets—such as solar data or data with known injected planetary and/or stellar signals—to better understand method performance and whether planetary signals are preserved

HARPS-N Solar Radial-Velocity Variations Are Dominated By Large, Bright Magnetic Regions

State of the art radial-velocity (RV) exoplanet searches are currently limited by RV signals arising from stellar magnetic activity. We analyze solar observations acquired over a 3-year period during the decline of Carrington Cycle 24 to test models of RV variation of Sun-like stars. A purpose-built solar telescope at the High Accuracy Radial velocity Planet Searcher for the Northern hemisphere (HARPS-N) provides disk-integrated solar spectra, from which we extract RVs and $\log{R'_{\rm HK}}$. The Solar Dynamics Observatory (SDO) provides disk-resolved images of magnetic activity. The Solar Radiation and Climate Experiment (SORCE) provides near-continuous solar photometry, analogous to a Kepler light curve. We verify that the SORCE photometry and HARPS-N $\log{R'_{\rm HK}}$ correlate strongly with the SDO-derived magnetic filling factor, while the HARPS-N RV variations do not. To explain this discrepancy, we test existing models of RV variations. We estimate the contributions of the suppression of convective blueshift and the rotational imbalance due to brightness inhomogeneities to the observed HARPS-N RVs. We investigate the time variation of these contributions over several rotation periods, and how these contributions depend on the area of active regions. We find that magnetic active regions smaller than $60 \ \rm Mm^2$ do not significantly suppress convective blueshift. Our area-dependent model reduces the amplitude of activity-induced RV variations by a factor of two. The present study highlights the need to identify a proxy that correlates specifically with large, bright magnetic regions on the surfaces of exoplanet-hosting stars.Comment: 16 pages, 7 figures, accepted by Ap

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

The rise of digital direct-to-consumer advertising?: Comparison of direct-to-consumer advertising expenditure trends from publicly available data sources and global policy implications

BACKGROUND: Pharmaceutical marketing is undergoing a major shift in the United States, in part due to new transparency regulations under the healthcare reform act. Changes in pharmaceutical marketing practices include a possible shift from more traditional forms of direct-to-consumer advertising towards emerging use of Internet-based DTCA (“eDTCA”) given the growing importance of digital health or “eHealth.” Though legally allowed only in the U.S. and New Zealand, eDTCA poses novel regulatory challenges, as it can cross geopolitical boundaries and impact health systems and populations outside of these countries. METHODS: We wished to assess whether changes in DTCA and eDTCA expenditure trends was occurring using publicly available pharmaceutical marketing data. DTCA data was analyzed to compare trends in aggregate marketing expenditures and to assess if there were statistically significant differences in trends and magnitudes for data sources and DTCA sub-categories (including eDTCA). This was accomplished using regression lines of DTCA trend data and conducting pairwise comparisons of regression coefficients using t-tests. Means testing was utilized for comparing magnitude of DTCA expenditure. RESULTS: Data from multiple data sources indicate that aggregate DTCA expenditures have slightly declined during the period from 2005–2009 and are consistent with results from other studies. For DTCA sub-categories, television remained the most utilized form of DTCA, though experienced trends of declining expenditures (−13.2 %) similar to other traditional media platforms such as radio (−30.7 %) and outdoor ads (−12.1 %). The only DTCA sub-category that experienced substantial increased expenditures was eDTCA (+109.0 %) and it was the only medium that had statistically significant differences in its marketing expenditure trends compared to other DTCA sub-categories. CONCLUSIONS: Our study indicates that traditional DTCA marketing may be on the decline. Conversely, the only DTCA sub-category that experienced significant increases was eDTCA. However, to fully understand this possible shift to “digital” DTCA, improvements in publicly available DTCA data sources are necessary to confirm changing trends and validate existing data. Hence, utilizing the newly implemented U.S. physician-payment expenditure transparency requirements, we advocate for the mandatory disclosure of DTCA/eDTCA in order to inform future domestic and international health policy efforts regarding appropriate regulation of pharmaceutical promotion