Total Colony-Forming Units Are a Strong, Independent Predictor of Neutrophil and Platelet Engraftment after Unrelated Umbilical Cord Blood Transplantation: A Single-Center Analysis of 435 Cord Blood Transplants

Graft failure occurs in approximately 20% of patients after unrelated umbilical cord blood transplantation (UCBT). This could be because of inadequate potency of the cord blood unit (CBU). To this end, we investigated the impact of graft characteristics on engraftment and survival of 435 primarily pediatric (median age: 5.3 years) patients receiving a single-unit unrelated UCBT after myeloablative conditioning from 2000 to 2008. Pre-cryopreservation (pre-cryo) graft characteristics were provided by the banks. Post-thaw parameters were measured on dextran/albumin-washed grafts. Post-thaw recovery of the colony-forming unit (CFU), a biological assay reflecting functional viability of the cord blood cells was the lowest percent age (median 21.2%, mean 36.5%) of the pre-cryo value, regardless of the bank of origin. The cumulative incidences of neutrophil and platelet engraftment were 76.9% (95%, confidence interval [CI], 71.3%-82.5%) and 55% (95% CI, 49.3%-60.7%), respectively. Univariate and separate multivariate models using pre-cryo and post-thaw datasets including clinical parameters identified predictors of engraftment and survival. In multivariate modeling, higher CFU dosing was the only pre-cryo graft characteristic predictive of neutrophil (P = .0024) and platelet engraftment (P = .0063). In the post-thaw model, CFU dose best predicted neutrophil and platelet engraftment (both P < .0001). Comparatively, CD34+ and total nucleated cell (TNC) were only weakly predictive in post-thaw neutrophil and platelet engraftment models, respectively. In conclusion, CFU dose is a strong independent predictor of engraftment after unrelated UCBT and should be used to assess potency when selecting CBUs for transplantation

IACHEC CROSS-CALIBRATION OF CHANDRA, NuSTAR, SWIFT, SUZAKU, XMM-NEWTON WITH 3C 273 ANDPKS 2155-304

On behalf of the International Astronomical Consortium for High Energy Calibration, we present results from the cross-calibration campaigns in 2012 on 3C 273 and in 2013 on PKS 2155-304 between the then active X-ray observatories Chandra, NuSTAR, Suzaku, Swift, and XMM-Newton. We compare measured fluxes between instrument pairs in two energy bands, 1–5 keV and 3–7 keV, and calculate an average cross-normalization constant for each energy range. We review known cross-calibration features and provide a series of tables and figures to be used for evaluating cross-normalization constants obtained from other observations with the above mentioned observatories.United States. National Aeronautics and Space Administration (Smithsonian Astrophysical Observatory. Contract SV3-73016)United States. National Aeronautics and Space Administration. (Grant NNX09AE58G

Generalized Sums over Histories for Quantum Gravity I. Smooth Conifolds

This paper proposes to generalize the histories included in Euclidean functional integrals from manifolds to a more general set of compact topological spaces. This new set of spaces, called conifolds, includes nonmanifold stationary points that arise naturally in a semiclasssical evaluation of such integrals; additionally, it can be proven that sequences of approximately Einstein manifolds and sequences of approximately Einstein conifolds both converge to Einstein conifolds. Consequently, generalized Euclidean functional integrals based on these conifold histories yield semiclassical amplitudes for sequences of both manifold and conifold histories that approach a stationary point of the Einstein action. Therefore sums over conifold histories provide a useful and self-consistent starting point for further study of topological effects in quantum gravity. Postscript figures available via anonymous ftp at black-hole.physics.ubc.ca (137.82.43.40) in file gen1.ps.Comment: 81pp., plain TeX, To appear in Nucl. Phys.

The Grizzly, December 6, 2007

Students: Fresh Produce on Sale Now • Relay for Life Kicks Off in Fanfare • Wings for Wishes Hosts Competitive Eating Contest • Balancing Act: Avoiding Weight Gain in College • Beer and the Bedroom • Soulja Boy to the Rescue • Trappe Tavern: It\u27s a Trap! • Hell is Other People • Ursinus Student Video Blogs in Japan • Opinions: Why Terrorists Do What They Do; New Homepage Long Overdue • Bears Off to a 1-0 Start in League • Fresh Faces on the Women\u27s Court • Preview of UC Winter Sportshttps://digitalcommons.ursinus.edu/grizzlynews/1752/thumbnail.jp

The Grizzly, October 25, 2007

Training for the Mind: Enter the Meditation World • Feel the Heat with Latin Dance Classes • Philly\u27s Finest Choreographs UCDC • Express Bus 93 Speeds Up Access to Center City • Ursinus College Bookstore Backs Edun Live Initiative • Understanding Pre-Menstrual Dysphoric Disorder (PMDD) • Ten Minutes Will Do • Four Students Nominated for Watson Fellowship • Book Review: Reasons to Live by Amy Hempel • Opinions: Genocide: Then and Now; Show at the Electric Factory; Dumbledore Out of the Broom Closet; When Arms Trade Goes Awry • New Season: Men\u27s Soccer • UC Training Room has a New Facehttps://digitalcommons.ursinus.edu/grizzlynews/1747/thumbnail.jp

IACHEC Cross-Calibration of Chandra, NuSTAR, Swift, Suzaku, and XMM-Newton with 3C 273 and PKS 2155-304

On behalf of the International Astronomical Consortium for High Energy Calibration, we present results from the cross-calibration campaigns in 2012 on 3C 273 and in 2013 on PKS 2155-304 between the then active X-ray observatories Chandra, NuSTAR, Suzaku, Swift, and XMM-Newton. We compare measured fluxes between instrument pairs in two energy bands, 1–5 keV and 3–7 keV, and calculate an average cross-normalization constant for each energy range. We review known cross-calibration features and provide a series of tables and figures to be used for evaluating cross-normalization constants obtained from other observations with the above mentioned observatories

Early and late outcomes after cord blood transplantation for pediatric patients with inherited leukodystrophies

Leukodystrophies (LD) are devastating inherited disorders leading to rapid neurological deterioration and premature death. Hematopoietic stem cell transplantation (HSCT) can halt disease progression for selected LD. Cord blood is a common donor source for transplantation of these patients because it is rapidly available and can be used without full HLA matching. However, precise recommendations allowing care providers to identify patients who benefit from HSCT are lacking. In this study, we define risk factors and describe the early and late outcomes of 169 patients with globoid cell leukodystrophy, X-linked adrenoleukodystrophy, and metachromatic leukodystrophy undergoing cord blood transplantation (CBT) at an European Society for Blood and Marrow Transplantation center or at Duke University Medical Center from 1996 to 2013. Factors associated with higher overall survival (OS) included presymptomatic status (77% vs 49%; P = .006), well-matched (80 preCBT, 50% remained stable, 20% declined to 60 to 80, and 30% to, 60. Overall, an encouraging OS was found for LD patients after CBT, especially for those who are presymptomatic before CBT and received adequately dosed grafts. Early identification and fast referral to a specialized center may lead to earlier treatment and, subsequently, to improved outcomes

Marine Benthic Diatoms Contain Compounds Able to Induce Leukemia Cell Death and Modulate Blood Platelet Activity

In spite of the high abundance and species diversity of diatoms, only a few bioactive compounds from them have been described. The present study reveals a high number of mammalian cell death inducing substances in biofilm-associated diatoms sampled from the intertidal zone. Extracts from the genera Melosira, Amphora, Phaeodactylum and Nitzschia were all found to induce leukemia cell death, with either classical apoptotic or autophagic features. Several extracts also contained inhibitors of thrombin-induced blood platelet activation. Some of this activity was caused by a high content of adenosine in the diatoms, ranging from 0.07 to 0.31 μg/mg dry weight. However, most of the bioactivity was adenosine deaminase-resistant. An adenosine deaminase-resistant active fraction from one of the extracts was partially purified and shown to induce apoptosis with a distinct phenotype. The results show that benthic diatoms typically found in the intertidal zone may represent a richer source of interesting bioactive compounds than hitherto recognized

Genetic risk and a primary role for cell-mediated immune mechanisms in multiple sclerosis.

Multiple sclerosis is a common disease of the central nervous system in which the interplay between inflammatory and neurodegenerative processes typically results in intermittent neurological disturbance followed by progressive accumulation of disability. Epidemiological studies have shown that genetic factors are primarily responsible for the substantially increased frequency of the disease seen in the relatives of affected individuals, and systematic attempts to identify linkage in multiplex families have confirmed that variation within the major histocompatibility complex (MHC) exerts the greatest individual effect on risk. Modestly powered genome-wide association studies (GWAS) have enabled more than 20 additional risk loci to be identified and have shown that multiple variants exerting modest individual effects have a key role in disease susceptibility. Most of the genetic architecture underlying susceptibility to the disease remains to be defined and is anticipated to require the analysis of sample sizes that are beyond the numbers currently available to individual research groups. In a collaborative GWAS involving 9,772 cases of European descent collected by 23 research groups working in 15 different countries, we have replicated almost all of the previously suggested associations and identified at least a further 29 novel susceptibility loci. Within the MHC we have refined the identity of the HLA-DRB1 risk alleles and confirmed that variation in the HLA-A gene underlies the independent protective effect attributable to the class I region. Immunologically relevant genes are significantly overrepresented among those mapping close to the identified loci and particularly implicate T-helper-cell differentiation in the pathogenesis of multiple sclerosis