Transgressions and expressions: Affective facial muscle activity predicts moral judgments

Recent investigations into morality suggest that affective responses may precede moral judgments. The present study investigated, first, whether individuals show specific facial affect in response to moral behaviors and, second, whether the intensity of facial affect predicts subsequent moral judgments. Muscle activity relating to disgust (levator labii), anger (corrugator supercilii), and positive affect (zygomaticus major) was recorded while participants considered third-person statements describing good and bad behaviors across five foundations of morality (purity, fairness, harm, authority, and ingroup). Facial disgust was highest in response to purity violations, followed by fairness violations. In contrast, harm violations evoked anger expressions. Importantly, the extremity of subsequent moral judgments was predicted by facial affect, such that judgments about purity and fairness correlated with facial disgust, harm correlated with facial anger, and ingroup correlated with positive facial affect. These results demonstrate that individuals spontaneously exhibit domain-specific moral affect that allows inferences about their moral judgments. </jats:p

Photometric Properties of Long-period Variables in the Large Magellanic Cloud

Approximately four thousand light curves of red variable stars in the LMC were selected from the 2.3-years duration MOA database by a period analysis using the Phase Dispersion Minimization method. Their optical features (amplitudes, periodicities, position in CMD) were investigated. Stars with large amplitues and high periodicities were distributed on the only one strip amongst multiple structure on the LMC period-luminosity relation. In the CMD, the five strips were located in the order of the period. The stars with characterized light curves were also discussed.Comment: 8 pages, 5 figures, Proceeding of WS on Mass-Losing Pulsating Stars and Their Circumstellar Matter, Sendai, Japa

PageRank in scale-free random graphs

We analyze the distribution of PageRank on a directed configuration model and show that as the size of the graph grows to infinity it can be closely approximated by the PageRank of the root node of an appropriately constructed tree. This tree approximation is in turn related to the solution of a linear stochastic fixed point equation that has been thoroughly studied in the recent literature

Application of Qualitative Methods in Health Research: An Overview

Qualitative research is type of formative research that includes specialized techniques for obtaining in-depth responses about what people think and how they feel. It is seen as the research that seeks answer to the questions in the real world. Qualitative researchers gather what they see, hear, read from people and places, from events and activities, with the purpose to learn about the community and to generate new understanding that can be used by the social world. Qualitative research have often been conducted to answer the question “why” rather than “what”. A purpose of qualitative research is the construction of new understanding. Here, we present an overview of application of qualitative methods in health research. We have discussed here the different types of qualitative methods and how we and others have used them in different settings/scenarios; sample size and sampling techniques; analysis of qualitative data; validity in qualitative research; and ethical issues

Dual state-parameter estimation of hydrological models using ensemble Kalman filter

Hydrologic models are twofold: models for understanding physical processes and models for prediction. This study addresses the latter, which modelers use to predict, for example, streamflow at some future time given knowledge of the current state of the system and model parameters. In this respect, good estimates of the parameters and state variables are needed to enable the model to generate accurate forecasts. In this paper, a dual state-parameter estimation approach is presented based on the Ensemble Kalman Filter (EnKF) for sequential estimation of both parameters and state variables of a hydrologic model. A systematic approach for identification of the perturbation factors used for ensemble generation and for selection of ensemble size is discussed. The dual EnKF methodology introduces a number of novel features: (1) both model states and parameters can be estimated simultaneously; (2) the algorithm is recursive and therefore does not require storage of all past information, as is the case in the batch calibration procedures; and (3) the various sources of uncertainties can be properly addressed, including input, output, and parameter uncertainties. The applicability and usefulness of the dual EnKF approach for ensemble streamflow forecasting is demonstrated using a conceptual rainfall-runoff model. © 2004 Elsevier Ltd. All rights reserved

User's guide to Monte Carlo methods for evaluating path integrals

We give an introduction to the calculation of path integrals on a lattice, with the quantum harmonic oscillator as an example. In addition to providing an explicit computational setup and corresponding pseudocode, we pay particular attention to the existence of autocorrelations and the calculation of reliable errors. The over-relaxation technique is presented as a way to counter strong autocorrelations. The simulation methods can be extended to compute observables for path integrals in other settings

Quantification of apolipoprotein E receptors in human brain-derived cell lines by real-time polymerase chain reaction

Apolipoprotein (apo) E4 is a risk factor for Alzheimer's disease (AD) and other neurodegenerative diseases, compared to wild-type apoE3. The mechanism(s) is unknown. One possibility, demonstrated in peripheral tissue cell lines, is that apoE stimulates nitric oxide synthase (NOS) via a receptor-dependent signalling pathway and that apoE4 generates inappropriate amounts of nitric oxide (NO) compared to apoE3. Prior to biochemical investigations, we have quantified the expression of several candidate receptor genes, including low-density lipoprotein-receptor (LDL-r) family members and scavenger receptor class B, types I and II (SR-BI/II), as well as the three NOS isoenzymes and protein kinase B (Akt), in 38 human cell lines, of which 12 derive from brain. Expression of apoE receptor 2 (apoER2), a known signalling receptor in brain, was readily detected in SH-SY-5Y and CCF-STTG1 cells, common models of neurons and astrocytes, respectively, and was highest in H4 neuroglioma, NT-2 precursor cells and IMR-32 neuroblastoma cells. Transcripts of the other lipoprotein receptors were widely, but variably, distributed across the different cell types. Of particular note was the predominant expression of SR-BII over SR-BI in many of the brain-derived cells. As the C-terminus of SR-BII, like apoER2, contains potential SH3 signalling motifs, we suggest that in brain SR-BII functions as a signal transducer receptor. (c) 2004 Elsevier Inc. All rights reserved

Lipid peroxidation is essential for α-synuclein-induced cell death.

Parkinson's disease is the second most common neurodegenerative disease and its pathogenesis is closely associated with oxidative stress. Deposition of aggregated α-synuclein (α-Syn) occurs in familial and sporadic forms of Parkinson's disease. Here, we studied the effect of oligomeric α-Syn on one of the major markers of oxidative stress, lipid peroxidation, in primary co-cultures of neurons and astrocytes. We found that oligomeric but not monomeric α-Syn significantly increases the rate of production of reactive oxygen species, subsequently inducing lipid peroxidation in both neurons and astrocytes. Pre-incubation of cells with isotope-reinforced polyunsaturated fatty acids (D-PUFAs) completely prevented the effect of oligomeric α-Syn on lipid peroxidation. Inhibition of lipid peroxidation with D-PUFAs further protected cells from cell death induced by oligomeric α-Syn. Thus, lipid peroxidation induced by misfolding of α-Syn may play an important role in the cellular mechanism of neuronal cell loss in Parkinson's disease. We have found that aggregated α-synuclein-induced production of reactive oxygen species (ROS) that subsequently stimulates lipid peroxidation and cell death in neurons and astrocytes. Specific inhibition of lipid peroxidation by incubation with reinforced polyunsaturated fatty acids (D-PUFAs) completely prevented the effect of α-synuclein on lipid peroxidation and cell death

Permission form synopses to improve parents' understanding of research: a randomized trial.

ObjectiveWe hypothesized that, among parents of potential neonatal research subjects, an accompanying cover sheet added to the permission form (intervention) would increase understanding of the research, when compared to a standard form (control).Study designThis pilot study enrolled parents approached for one of two index studies: one randomized trial and one observational study. A one-page cover sheet described critical study information. Families were randomized 1:1 to receive the cover sheet or not. Objective and subjective understanding and satisfaction were measured.ResultsThirty-two parents completed all measures (17 control, 15 intervention). There were no differences in comprehension score (16.8±5.7 vs 16.3±3.5), subjective understanding (median 6 vs 6.5), or overall satisfaction with consent (median 7 vs 6.5) between control and intervention groups (all P&gt;0.50).ConclusionA simplified permission form cover sheet had no effect on parents' understanding of studies for which their newborns were being recruited