Metabolic changes during he transition period

We used four ruminally fistulated, multiparous, pregnant Holstein cows to measure changes in concentrations of plasma metabolite as the dairy cow transitions from one lactation to the next. Diets consisted of typical far-off and close-up diets, a late lactation diet containing wet corn gluten feed (20% DM), and an alfalfa hay-corn silage based early lactation diet. Calculated NEL (Mcal/lb), measured crude protein (%), and diet digestibilities (%; based on steers fed at 2% of BW) were 0.78, 18.7, 74.1; 0.70, 11.5, 66.2; 0.74, 15.6, 71.0; 0.73, 18.4, 70.7 for late lactation, far-off dry, close-up dry, and early lactation diets, respectively. Blood samples were obtained on day 79 prior to calving and weekly thereafter until calving and on days 1, 3, 5, 7, 15, 20, 25, 30, 60, and 90 after calving. Cows gained body weight and condition during the dry period, peaked just prior to calving, and lost weight and condition steadily through the first 11 weeks of lactation. Calculated energy balance was negative during the first 3 weeks of lactation. Plasma concentrations of non-esterified fatty acids (NEFA), glucose, and insulin to glucagon ratio remained fairly stable during the dry period. Plasma glucose increased just before calving, decreased markedly during early lactation, then increased and stabilized by day 30 of lactation. Plasma NEFA concentrations increased at calving and were elevated during early lactation, then returned to prepartum concentrations by day 30 of lactation. The insulin to glucagon ratio decreased just prior to calving, continued to decrease until day 7 of lactation, and then remained stable until the end of the trial. Changes in diet and intake affected plasma urea nitrogen, which decreased as dietary protein decreased during the far-off period, decreased with intake during the close-up period, and increased after calving consistent with the higher dietary protein and increase in dry matter intake. Most of the observed metabolic adaptations reflected the energy status of the cow with large shifts occurring around parturition. Certainly, some of the hormones associated with calving can initiate metabolic events favorable to lactation, but the changes in energy balance and nutrient supply support the continued diversion of nutrients to the mammary gland. These data support the concept that dairy cows experience a period of increased tissue mobilization from approximately 2 days prior to calving until 30 days after calving. In conclusion, a number of metabolic adaptations occur in transition dairy cows that provide clues to improve feeding and management guidelines.; Dairy Day, 2002, Kansas State University, Manhattan, KS, 2002

Changes in rumen capacity of dairy cows during the periparturient period

Four-ruminally fistulated, multiparous, pregnant Holstein cows were studied to characterize ruminal adaptations during the transition from gestation to lactation. Cows were fed typical far-off and close-up diets, a late lactation diet containing wet corn gluten feed (20% DM), and an alfalfa hay, corn silage based early lactation diet. Ruminal measurements were obtained 72 (late lactation), 51 (far-off dry), 23 and 9 (close-up dry) days before expected parturition and 6, 20, and 34 days postpartum. Measurements included total fill, dry matter fill, fluid fill, and water-holding capacity of the rumen. Dry matter intake and milk production data were collected daily and body weight and body condition were determined weekly. Body weights and condition increased during the dry period, whereas intake as a percentage of body weight decreased. Ruminal water holding capacity, an indicator of rumen capacity, increased linearly from late lactation to 34 days postpartum. These data suggest rumen capacity is not the causative factor of intake depression in dairy cows during the final 3 wk of gestation.; Dairy Day, 2001, Kansas State University, Manhattan, KS, 2001

Characteristic changes of ruminal fermentation in transition dairy cows

Four-ruminally fistulated, multiparous, pregnant Holstein cows were used to delineate changes in ruminal fermentation in dairy cows as they experienced the transition from one lactation to the next. Diets consisted of typical far-off and close-up diets, a late lactation diet containing wet corn gluten feed (20% DM) and an alfalfa hay-corn silage based early lactation diet. Calculated NEL (Mcal/lb), measured crude protein (%), and diet digestibilities (%; based on steers fed at 2% of BW) were: 0.78, 18.7, 74.1; 0.70, 11.5, 66.2; 0.74, 15.6, 71.0; 0.73, 18.4, 70.7 for late lactation, far-off dry, close-up dry, and early lactation diets, respectively. Ruminal measurements were taken on days 72 (late lactation), 51 (faroff), 23, and 9 (close-up dry) before calving and on days 6, 20, 34, 48, 62, 76, and 90 days after calving. Ruminal samples were collected at hours 0, 3, 6, 9, and 12 after feeding on each sampling date. Major shifts in ruminal fermentations occurred when the close-up diet was consumed before calving and in concert with an increase in DM intake during the first 48 days of lactation. Dry matter digestibility increased after cows were switched to the close-up diet and continued this trend through day 6 postpartum. Ruminal pH decreased and total volatile fatty acids, peptides, and free amino acids increased after cows were switched to the early lactation diet. These data support the concept that alterations in ruminal fermentation reflect changes in both diet and intake.; Dairy Day, 2002, Kansas State University, Manhattan, KS, 2002

Comparison of three fresh cow feeding programs

We evaluated the impact on performance of top dressing a based total mixed ration (TMR) with long-stem alfalfa hay with or without additional dry-rolled corn to the lactating cow diet during the first 5 days postpartum. The three dietary treatments and numbers of cows assigned to each diet were: 1) total mixed ration (TMR; n = 19); 2) TMR + long-stem alfalfa hay (TMR + A; n= 20); and 3) TMR + long-stemmed alfalfa hay + dry-rolled corn (TMR + A + C; n = 20). Top dressing the lactating TMR with long-stem alfalfa hay with or without dry-rolled corn did not reduce the incidence of metabolic disorders in early lactating cows. Six cows, two on each diet, were treated for displaced abomasums. Cows consuming only the TMR lost slightly more body weight during the first 30 days after calving compared to cows fed the other diets. Milk and energy corrected milk (ECM) yields were similar among diets. Fat, protein, and urea nitrogen content in milk were not different among dietary treatments. Lactose content in milk was greater for cows consuming TMR + A than those consuming TMR or TMR + A + C. Concentrations of glucose and urea nitrogen in plasma were not affected by treatment during the initial 5 days of lactation. Concentrations of glucose and urea nitrogen on days 2 and 3 were less for multiparous cows consuming TMR than for multiparous cows consuming TMR + A. Rumen contractions during the first 5 days of lactation were not different among diets. Top dressing the lactating TMR with long-stem alfalfa hay with or without dry-rolled corn was not beneficial in this study. On a dry matter basis, the lactating TMR contained 22% chopped alfalfa hay, 10% corn silage, 20% wet corn gluten feed, 9% whole fuzzy cottonseed, 7.1% expeller soybean meal, 27.4% ground shelled corn, 1.2% molasses, 1.3% Menhaden fishmeal, and 2.0% mineral-vitamin premix. Cows fed diets containing corn silage as the predominant fiber source may respond differently.; Dairy Day, 2003, Kansas State University, Manhattan, KS, 2003

Comlete lactational performance of cows fed wet corn gluten feed and pellet consisting of raw soybean hulls and corn steep liquor

We evaluated the effect of wet corn gluten feed and a novel product containing raw soybean hulls and corn steep liquor on performance in lactating dairy cows. Forty-six multiparous Holstein cows were used in a randomized incomplete block design. Cows were housed in tie stalls for the first 13 weeks of lactation and moved to group pens for the remainder of the study. Cows were blocked by calving date and assigned to control, wet corn gluten feed (20% of diet DM), or the novel product (20% of diet DM). Diets were administered as total mixed rations at the first feeding postpartum. Control contained (DM basis) 30% alfalfa hay, 15% corn silage, 32% corn, 9.3% whole cottonseed, 4.4% solvent soybean meal (SBM), 3.3% expeller SBM, 1.3% fish meal, 1% wet molasses, and 3.7% vitamins/minerals. Wet corn gluten feed replaced 10% alfalfa hay, 5% corn silage, 5% corn grain, and expeller SBM replaced solvent SBM to maintain diet rumen undegradable protein. The novel product replaced 10% alfalfa hay, 5% corn silage, 3% solvent SBM, and 2% corn. Diet crude protein % and energy density (Mcal/lb, NEL) for control, wet corn gluen feed, and the novel product were 18.4, 0.73; 18.2, 0.75; 18.5, 0.73; respectively. Milk, energy corrected milk, dry matter intake, and production efficiency (ratio of milk to DM intake) did not differ among diets during the first 91 days of lactation, but there was a diet by week interaction for production efficiency. Cows fed control were more efficient during the first 2 weeks postpartum than cows fed wet corn gluten feed and the novel product, likely due to increased fat mobilization from adipose tissue because intake as a percent of body weight was less for cows fed control. During weeks 3 through 14 postpartum, wet corn gluten feed and the novel product improved milk, energy corrected milk, and milk component yield, and production efficiency. Inclusion of wet corn gluten feed and the novel product at 20% of dietary DM as a partial replacement for alfalfa hay, corn silage, corn grain, and SBM in diets fed to lactating dairy cattle supported performance during early lactation and improved performance during mid and late lactation. In addition, combining wet corn gluten feed or the novel product with corn silage and alfalfa hay maintained milk fat yields, thereby demonstrating that they can serve as effective sources of fiber when fed at 20% of dietary DM. Improved performance attributed to wet corn gluten feed and the novel product is due to factors other than improved digestibility of the diets. These results indicate that wet corn gluten feed and the novel product can serve as alternative feedstuffs in diets fed to lactating dairy cattle.; Dairy Day, 2002, Kansas State University, Manhattan, KS, 2002

Changes in ruminal microbial populations in transition dairy cows

We used four ruminally fistulated, multiparous, pregnant Holstein cows to delineate microbial adaptations in dairy cows as they experienced the transition from one lactation to the next. Diets consisted of typical far-off and close-up diets, a late lactation diet containing wet corn gluten feed (20% DM) and an alfalfa hay-corn silage based early lactation diet. Calculated NEL (Mcal/lb), measured crude protein (%), and diet digestibilities (%; based on steers fed at 2% of BW) were: 0.78, 18.7, 74.1; 0.70, 11.5, 66.2; 0.74, 15.6, 71.0; 0.73, 18.4, 70.7 for late lactation, far-off dry, close-up dry, and early lactation, respectively. Microbial samples were obtained on days 72 (late lactation), 51 (far-off dry), 23, and 9 (close-up dry) prepartum and days 6, 20, 34, 48, 62, 76, and 90 postpartum. We analyzed ruminal samples for ciliated protozoa and viable counts of bacteria and fungi. Changing from a high forage to a high concentrate diet impacted bacterial counts less than ciliated protozoal and fungal counts. Switching diets from high concentrate to high forage increased ciliated protozoa and fungal counts, and counts decreased when diets were switched from high forage to high concentrate. Bacterial and ciliated protozoa counts increased in early lactation and decreased as cows approached peak dry matter intake. Dietary changes with the onset of lactation led to virtual disappearance of fungi from the rumen. In general, ruminal microbial populations of dairy cows respond to changes in diet and intake. Changes in diet affected populations of protozoa and fungi, whereas changes in intake affected populations of bacteria, protozoa, and fungi.; Dairy Day, 2002, Kansas State University, Manhattan, KS, 2002

Intake and performance of dairy cows fed wet corn gluten feed during the periparturient period

Eight primiparous and nine multiparous Holstein cows were used in a randomized block design to determine the effect of wet corn gluten feed in the diet during the last 21 days of gestation on dry matter intake and early postpartum performance. Multilactation cows fed wet corn gluten feed maintained a higher dry matter intake and intake as a percentage of body weight during the last week before calving than cows fed the control diet. First-lactation cows fed wet corn gluten feed consumed less dry matter, both total and as a percentage of body weight, across calving than first-lactation cows fed the control diet. Milk, milk components, and blood metabolites were not influenced by diet. Wet corn gluten feed may help alleviate the depression in intake typically observed during late gestation for multiparous but not primiparous cows.; Dairy Day, 2000, Kansas State University, Manhattan, KS, 2000

XAS study of the local environment of impurities in doped TiO2 thin films

In this work we present an X-ray Absorption Spectroscopy characterization of the local environment of the impurity in room temperature ferromagnetic anatase TiO2 thin films doped with Co, Ni, Cu, or Zn, deposited on LaAlO3 substrate by Pulsed Laser Deposition. It was found that there is a considerable amount of impurity atoms substituting Ti in TiO2 anatase, although the presence of metal transition monoxide clusters can not be discarded. From our results we infer that the observed room temperature ferromagnetism of the samples could be assigned to the metal transition atoms replacing Ti in TiO2 anatase.Comment: 4 pages, 3 figures, 1 table, Physica B (in press

Relationship among concentrations of milk urea nitrogen and plasma urea nitrogen and feeding time

Eight Holstein cows were used to determine the relationship among milk urea nitrogen (MUN), plasma urea nitrogen (PUN), and feeding time. We first established that MUN concentrations were similar in concentration among quarters by comparing milk samples from each quarter just before milking. In order to determine if collecting a sample of milk from a quarter influenced the MUN in samples taken later, samples were obtained from the right front quarter (RF) at 2, 4, 6, and 8 hr after the AM milking and from the left front quarter (LF), right rear (RR), and left rear (LR) at 4, 6, and 8 h after the AM milking, respectively. The MUN in samples obtained from RF at 4 hr was lower (P\u3c0.01) than corresponding samples taken from LF, but samples from RF at 6 and 8 hr did not differ from corresponding samples obtained from RR and LR. We concluded that by 6 hr, the effect of previous milking on MUN concentration disappeared because of dilution. To determine the influence of feeding time on MUN concentrations, cows were fed half of their normal PM feeding, injected with oxytocin at the subsequent AM milking to reduce residual milk, and offered surplus feed after the AM milking. Milk samples were collected at 2, 4, 6, 8, 10, and 12 hr after feeding from RF, LF, RR, LR, RF, and LF quarters, respectively. Blood samples were obtained from the coccygeal vein at hourly intervals after feeding with the last sample collected 12 hr after feeding. The MUN concentrations at 2, 4, 6, and 8 hr were similar. The MUN at 10 hr was similar to those at 2 and 8 hr, less than that at 4 and 6 hr, and greater than that for the 12 hr sample. Concentrations of PUN peaked at 2 hr postfeeding, then gradually declined through 12 hr postfeeding. The MUN peaked at 6 hr postfeeding and then declined. Time after feeding significantly influenced PUN and MUN concentrations.; Dairy Day, 2000, Kansas State University, Manhattan, KS, 2000

Sampling techniques for and interpretation of milk urea nitrogen concentration

Representative MUN values can be obtained by testing a milk sample before milking, at AM or PM milking, or with an in-line siphon sampling device. MUN values obtained from homogenous milking strings are as accurate as an average MUN value obtained by sampling each cow in the string. Bulk tank sampling is not advisable because of the variation in MUN caused by stage of lactation. Small herds that feed a single TMR should use the average MUN from cows between 60 and 200 days in milk. Monthly sampling is recommended to build a database. The effect of diet changes on MUN can be assessed within 7 days.; Dairy Day, 1998, Kansas State University, Manhattan, KS, 1998