41 research outputs found

    Analysis of the Brinkman-Forchheimer equations with slip boundary conditions

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    In this work, we study the Brinkman-Forchheimer equations driven under slip boundary conditions of friction type. We prove the existence and uniqueness of weak solutions by means of regularization combined with the Faedo-Galerkin approach. Next we discuss the continuity of the solution with respect to Brinkman's and Forchheimer's coefficients. Finally, we show that the weak solution of the corresponding stationary problem is stable

    Gene Disruption in Scedosporium aurantiacum: Proof of Concept with the Disruption of SODC Gene Encoding a Cytosolic Cu,Zn-Superoxide Dismutase

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    Scedosporium species are opportunistic pathogens responsible for a large variety of infections in humans. An increasing occurrence was observed in patients with underlying conditions such as immunosuppression or cystic fibrosis. Indeed, the genus Scedosporium ranks the second among the filamentous fungi colonizing the respiratory tracts of the CF patients. To date, there is very scarce information on the pathogenic mechanisms, at least in part because of the limited genetic tools available. In the present study, we successfully developed an efficient transformation and targeted gene disruption approach on the species Scedosporium aurantiacum. The disruption cassette was constructed using double-joint PCR procedure, and resistance to hygromycin B as the selection marker. This proof of concept was performed on the functional gene SODC encoding the Cu,Zn-superoxide dismutase. Disruption of the SODC gene improved susceptibility of the fungus to oxidative stress. This technical advance should open new research areas and help to better understand the biology of Scedosporium species

    Crystal field effects and shielding of uranium ions

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    La séparation du niveau fondamental de l'ion libre U4+ a été calculée dans un environnement octahédral et cubique en tenant compte du mélange des différents termes spectroscopiques. Les facteurs d'écrantage de Sternheimer ont été calculés pour les ions U4+ et U3+.The splitting of the lowest level of the free ion U4+ has been calculated in an octahedral and cubic environment taking into account the mixture of different spectroscopic terms. The Sternheimer shielding factors have been calculated for the ions U4+ and U3+
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