143 research outputs found
TCR V α- and V Ă-Gene Segment Use in T-Cell Subcultures Derived from a Type-III Bare Lymphocyte Syndrome Patient Deficient in MHC Class-II Expression
Previously, we and others have shown that MHC class-II deficient humans have greatly
reduced numbers of CD4+CD8â peripheral T cells. These type-III Bare Lymphocyte
Syndrome patients lack MHC class-II and have an impaired MHC class-I antigen
expression. In this study, we analyzed the impact of the MHC class-II deficient
environment on the TCR V-gene segment usage in this reduced CD4+CD8â T-cell
subset. For these studies, we employed TcR V-region-specific monoclonal antibodies
(mAbs) and a semiquantitative PCR technique with V α and V à amplimers, specific for
each of the most known V α- and V Ă;-gene region families. The results of our studies
demonstrate that some of the V α-gene segments are used less frequent in the
CD4+CD8â T-cell subset of the patient, whereas the majority of the TCR V α- and
V Ă-gene segments investigated were used with similar frequencies in both subsets in the
type-III Bare Lymphocyte Syndrome patient compared to healthy control family
members. Interestingly, the frequency of TcR V α12 transcripts was greatly diminished
in the patient, both in the CD4+CD8â as well as in the CD4âCD8+ compartment,
whereas this gene segment could easily be detected in the healthy family controls. On
the basis of the results obtained in this study, it is concluded that within the reduced
CD4+CD8â T-cell subset of this patient, most of the TCR V-gene segments tested for are
employed. However, a skewing in the usage frequency of some of the V α-gene segments
toward the CD4âCD8+ T-cell subset was noticeable in the MHC class-II deficient patient
that differed from those observed in the healthy family controls
A numerical study of tropical cross-tropopause transport by convective overshoots during the TROCCINOX golden day
International audienceObservations obtained during the Tropical Convection, Cirrus and Nitrogen Oxides (TROCCINOX) golden day have revealed the presence of ice particles up to 410 K (18.2 km) 2 km above the local tropopause. The case is investigated using a three-dimensional quadruply nested non-hydrostatic simulation and Meteosat Second Generation (MSG) observations. The simulation fairly well reproduces the measurements along the flight track. A reasonable agreement with MSG observations is also achieved: the 10.8-”m brightness temperature (BT) minimum of 187 K is reproduced (a value 6 K colder than the environmental cold-point temperature) as well as the positive BT difference between the 6.2- and 10.8-”m bands, an overshoot signature. The simulation produces several overshooting plumes up to 410 K yielding an upward transport of water vapour of a few tons per second across the tropical tropopause. The estimated mass flux agree with those derived from over tracer budgets indicating that convection transport mass across the tropopause
Regional lightning NOx sources during the TROCCINOX experiment
A lightning NOx (LiNOx) source has been implemented in the deep convection scheme of the Meso-NH mesoscale model following a mass-flux formalism coherent with the transport and scavenging of gases inside the convective
scheme. In this approach the vertical transport of NO inside clouds is calculated by the parameterization of deep convective transport, thus eliminating the need for apriori LiNOx profiles. Once produced inside the convective column, NO molecules are redistributed by updrafts and downdrafts and detrained in the environment when the conditions are favorable. The model was applied to three particular flights during the Tropical Convection, Cirrus and Nitrogen Oxides (TROCCINOX) campaign over the tropical area around Bauru on 3-4 March 2004. The convective activity during the three flights was investigated using brightness temperature at 10.7ÎŒm observed from GOES-12 satellite. The use of a model-to-satellite approach reveals that the simulation appears rather realistic compared to the observations. The diurnal cycle of the simulated brightness temperature, CAPE, number of IC flashes, NO entrainment flux are in phase, with a succession of three marked peaks at 18:00 UTC (15:00 LT). These simulated peaks precede the observed afternoon one by about three hours. Comparison of the simulated NOx with observations along the flight tracks show that the model reproduces well the observed NOx levels when the LiNOx source is applied. The budget of entrainment, detrainment and LiNOx convective fluxes shows that the majority of the NO detrained back to the environment comes from lightning source inside the convective columns. Entrainment of NO from the environment and vertical transport from the boundary layer were not significant during the episode. The troposphere is impacted by detrainment fluxes of LiNOx from 4 km altitude to 16 km with maximum values around 14 km altitude. Detrainment fluxes vary between 75 kg(N)/s during nighttime to 400 kg(N)/s at the times of maximun convective activity. Extrapolation of the regional LiNOx source would yield a global LiNOx production around 5.7 Tg(N)/year which is within the current estimates but should not hide the overestimation of the number of flash rates by the model
Interleukin 7 from Maternal Milk Crosses the Intestinal Barrier and Modulates T- Cell Development in Offspring
Background
Breastfeeding protects against illnesses and death in hazardous environments, an
effect partly mediated by improved immune function. One hypothesis suggests that
factors within milk supplement the inadequate immune response of the offspring,
but this has not been able to account for a series of observations showing that
factors within maternally derived milk may supplement the development of the
immune system through a direct effect on the primary lymphoid organs. In a
previous human study we reported evidence suggesting a link between IL-7 in
breast milk and the thymic output of infants. Here we report evidence in mice of
direct action of maternally-derived IL-7 on T cell development in the offspring.
Methods and Findings
 We have used recombinant IL-7 labelled with a fluorescent dye to trace the
movement in live mice of IL-7 from the stomach across the gut and into the
lymphoid tissues. To validate the functional ability of maternally derived IL-
7 we cross fostered IL-7 knock-out mice onto normal wild type mothers. Subsets
of thymocytes and populations of peripheral T cells were significantly higher
than those found in knock-out mice receiving milk from IL-7 knock-out mothers.
Conclusions/Significance Our study provides direct evidence that interleukin 7,
a factor which is critical in the development of T lymphocytes, when maternally
derived can transfer across the intestine of the offspring, increase T cell
production in the thymus and support the survival of T cells in the peripheral
secondary lymphoid tissue
Maternal Infection with Trypanosoma cruzi and Congenital Chagas Disease Induce a Trend to a Type 1 Polarization of Infant Immune Responses to Vaccines
Vaccines are of crucial importance to prevent morbidity and mortality due to infectious diseases in childhood. A modulation of the fetal/neonatal immune system (considered immature) toward Th1 or Th2 dominance could modify responses to vaccines administered in early life. T. cruzi is the agent of Chagas' disease, in Latin America currently infecting about 2 million women at fertile ages who are susceptible to transmitting the parasite to their fetus. In previous studies we showed that T. cruzi-infected mothers can induce a pro-inflammatory environment in their uninfected neonates (M+Bâ), whereas congenitally infected newborns (M+B+) are able to develop a pro-Th1 parasite-specific T cell response. In the present study, we analysed the cellular and/or antibody responses to Bacillus Calmette Guerin (BCG), hepatitis B birus (HBV), diphtheria and tetanus vaccines in 6- to 7-month-old infants living in Bolivia. M+Bâ infants produced more IFN-Îł in response to BCG, whereas M+B+ infants developed a stronger IFN-Îł response to hepatitis B, diphtheria and tetanus vaccines and enhanced antibody production to HBs antigen. These results show that both maternal infection with T. cruzi and congenital Chagas disease do not interfere with responses to BCG, hepatitis B, diphtheria and tetanus vaccines in the neonatal period and that T. cruzi infection in early life tends to favour type 1 immune responses to vaccinal antigens
Strong HIV-1-Specific T Cell Responses in HIV-1-Exposed Uninfected Infants and Neonates Revealed after Regulatory T Cell Removal
BACKGROUND: In utero transmission of HIV-1 occurs on average in only 3%â15% of HIV-1-exposed neonates born to mothers not on antiretroviral drug therapy. Thus, despite potential exposure, the majority of infants remain uninfected. Weak HIV-1-specific T-cell responses have been detected in children exposed to HIV-1, and potentially contribute to protection against infection. We, and others, have recently shown that the removal of CD4(+)CD25(+) T-regulatory (Treg) cells can reveal strong HIV-1 specific T-cell responses in some HIV-1 infected adults. Here, we hypothesized that Treg cells could suppress HIV-1-specific immune responses in young children. METHODOLOGY/PRINCIPAL FINDINGS: We studied two cohorts of children. The first group included HIV-1-exposed-uninfected (EU) as well as unexposed (UNEX) neonates. The second group comprised HIV-1-infected and HIV-1-EU children. We quantified the frequency of Treg cells, T-cell activation, and cell-mediated immune responses. We detected high levels of CD4(+)CD25(+)CD127(â) Treg cells and low levels of CD4(+) and CD8(+) T cell activation in the cord blood of the EU neonates. We observed HIV-1-specific T cell immune responses in all of the children exposed to the virus. These T-cell responses were not seen in the cord blood of control HIV-1 unexposed neonates. Moreover, the depletion of CD4(+)CD25(+) Treg cells from the cord blood of EU newborns strikingly augmented both CD4(+) and CD8(+) HIV-1-specific immune responses. CONCLUSIONS/SIGNIFICANCE: This study provides new evidence that EU infants can mount strong HIV-1-specific T cell responses, and that in utero CD4(+)CD25(+) T-regulatory cells may be contributing to the lack of vertical transmission by reducing T cell activation
Is the meiofauna a good indicator for climate change and anthropogenic impacts?
Our planet is changing, and one of the most pressing challenges facing the scientific community revolves around understanding how ecological communities respond to global changes. From coastal to deep-sea ecosystems, ecologists are exploring new areas of research to find model organisms that help predict the future of life on our planet. Among the different categories of organisms, meiofauna offer several advantages for the study of marine benthic ecosystems. This paper reviews the advances in the study of meiofauna with regard to climate change and anthropogenic impacts. Four taxonomic groups are valuable for predicting global changes: foraminifers (especially calcareous forms), nematodes, copepods and ostracods. Environmental variables are fundamental in the interpretation of meiofaunal patterns and multistressor experiments are more informative than single stressor ones, revealing complex ecological and biological interactions. Global change has a general negative effect on meiofauna, with important consequences on benthic food webs. However, some meiofaunal species can be favoured by the extreme conditions induced by global change, as they can exhibit remarkable physiological adaptations. This review highlights the need to incorporate studies on taxonomy, genetics and function of meiofaunal taxa into global change impact research
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