The Dipole Observed in the COBE DMR Four-Year Data

The largest anisotropy in the cosmic microwave background (CMB) is the $\approx 3$ mK dipole assumed to be due to our velocity with respect to the CMB. Using the four year data set from all six channels of the COBE Differential Microwave Radiometers (DMR), we obtain a best-fit dipole amplitude $3.358 \pm 0.001 \pm 0.023$ mK in the direction $(l,b)=(264\deg.31 \pm 0\deg.04 \pm 0\deg.16, +48\deg.05 \pm 0\deg.02 \pm 0\deg.09)$, where the first uncertainties are statistical and the second include calibration and combined systematic uncertainties. This measurement is consistent with previous DMR and FIRAS resultsComment: New and improved version; to be published in ApJ next mont

Correlated Errors in the COBE DMR Sky Maps

The {\it COBE} DMR sky maps contain low-level correlated noise. We obtain estimates of the amplitude and pattern of the correlated noise from three techniques: angular averages of the covariance matrix, Monte Carlo simulations of two-point correlation functions, and direct analysis of the DMR maps. The results from the three methods are mutually consistent. The noise covariance matrix of a DMR sky map is diagonal to an accuracy of better than 1\%. For a given sky pixel, the dominant noise covariance occurs with the ring of pixels at an angular separation of $60 \deg$ due to the $60 \deg$ separation of the DMR horns. The mean covariance at $60 \deg$ is $0.45\% ^{+0.18}_{-0.14}$ of the mean variance. Additionally, the variance in a given pixel is $0.7\%$ greater than would be expected from a single beam experiment with the same noise properties. Auto-correlation functions suffer from a $\sim 1.5\; \sigma$ positive bias at $60 \deg$ while cross-correlations have no bias. Published {\it COBE} DMR results are not significantly affected by correlated noise. COBE pre-print 94-Comment: 11 pages + 3 figures, post-script fil

Structural Characterization of Outer Membrane Components of the Type IV Pili System in Pathogenic Neisseria

Structures of the type IV pili secretin complexes from Neisseria gonorrhoeae and Neisseria meningitidis, embedded in outer membranes were investigated by transmission electron microscopy. Single particle averaging revealed additional domains not observed previously. Secretin complexes of N. gonorrhoeae showed a double ring structure with a 14-15-fold symmetry in the central ring, and a 14-fold symmetry of the peripheral ring with 7 spikes protruding. In secretin complexes of N. meningitidis, the spikes were absent and the peripheral ring was partly or completely lacking. When present, it had a 19-fold symmetry. The structures of the complexes in several pil mutants were determined. Structures obtained from the pilC1/C2 adhesin and the pilW minor pilin deletion strains were similar to wild-type, whereas deletion of the homologue of N. meningitidis PilW resulted in the absence of secretin structures. Remarkably, the pilE pilin subunit and pilP lipoprotein deletion mutants showed a change in the symmetry of the peripheral ring from 14 to 19 and loss of spikes. The pilF ATPase mutant also lost the spikes, but maintained 14-fold symmetry. These results show that secretin complexes contain previously unidentified large and flexible extra domains with a probable role in stabilization or assembly of type IV pili

4-Year COBE DMR Cosmic Microwave Background Observations: Maps and Basic Results

The cosmic microwave background radiation provides unique constraints on cosmological models. In this Letter we present a summary of the spatial properties of the cosmic microwave background radiation based on the full 4 years of COBE DMR observations, as detailed in a set of companion Letters. The anisotropy is consistent with a scale-invariant power law model and Gaussian statistics. With full use of the multi-frequency 4-year DMR data, including our estimate of the effects of Galactic emission, we find a power-law spectral index of $n=1.2\pm 0.3$ and a quadrupole normalization $Q_{rms-PS}=15.3^{+3.8}_{-2.8}$ $\mu$K. For $n=1$ the best-fit normalization is $Q_{rms-PS}\vert_{n=1}=18\pm 1.6$ $\mu$K. These values are consistent with both our previous 1-year and 2-year results. The results include use of the $\ell=2$ quadrupole term; exclusion of this term gives consistent results, but with larger uncertainties. The 4-year sky maps, presented in this Letter, portray an accurate overall visual impression of the anisotropy since the signal-to-noise ratio is ~2 per 10 degree sky map patch. The improved signal-to-noise ratio of the 4-year maps also allows for improvements in Galactic modeling and limits on non-Gaussian statistics.Comment: 11 pages plus 2 PostScript figures. Figures 2 and 4 are not included, but are available upon request to [email protected]. Submitted to The Astrophysical Journal (Letters

Localization of a 64-kDa phosphoprotein in the lumen between the outer and inner envelopes of pea chloroplasts

The identification and localization of a marker protein for the intermembrane space between the outer and inner chloroplast envelopes is described. This 64-kDa protein is very rapidly labeled by [γ-32P]ATP at very low (30 nM) ATP concentrations and the phosphoryl group exhibits a high turnover rate. It was possible to establish the presence of the 64-kDa protein in this plastid compartment by using different chloroplast envelope separation and isolation techniques. In addition comparison of labeling kinetics by intact and hypotonically lysed pea chloroplasts support the localization of the 64-kDa protein in the intermembrane space. The 64-kDa protein was present and could be labeled in mixed envelope membranes isolated from hypotonically lysed plastids. Mixed envelope membranes incorporated high amounts of 32P from [γ-32P]ATP into the 64-kDa protein, whereas separated outer and inner envelope membranes did not show significant phosphorylation of this protein. Water/Triton X-114 phase partitioning demonstrated that the 64-kDa protein is a hydrophilic polypeptide. These findings suggest that the 64-kDa protein is a soluble protein trapped in the space between the inner and outer envelope membranes. After sonication of mixed envelope membranes, the 64-kDa protein was no longer present in the membrane fraction, but could be found in the supernatant after a 110000 × g centrifugation

A guanosine 5′-triphosphate-dependent protein kinase is localized in the outer envelope membrane of pea chloroplasts

A guanosine 5-triphosphate (GTP)-dependent protein kinase was detected in preparations of outer chloroplast envelope membranes of pea (Pisum sativum L.) chloroplasts. The protein-kinase activity was capable of phosphorylating several envelope-membrane proteins. The major phosphorylated products were 23- and 32.5-kilo-dalton proteins of the outer envelope membrane. Several other envelope proteins were labeled to a lesser extent. Following acid hydrolysis of the labeled proteins, most of the label was detected as phosphoserine with only minor amounts detected as phosphothreonine. Several criteria were used to distinguish the GTP-dependent protein kinase from an ATP-dependent kinase also present in the outer envelope membrane. The ATP-dependent kinase phosphorylated a very different set of envelope-membrane proteins. Heparin inhibited the GTP-dependent kinase but had little effect upon the ATP-dependent enzyme. The GTP-dependent enzyme accepted phosvitin as an external protein substrate whereas the ATP-dependent enzyme did not. The outer membrane of the chloroplast envelope also contained a phosphotransferase capable of transferring labeled phosphate from [-32P]GTP to ADP to yield (-32P]ATP. Consequently, addition of ADP to a GTP-dependent protein-kinase assay resulted in a switch in the pattern of labeled products from that seen with GTP to that typically seen with ATP

Dipole Anisotropy in the COBE DMR First-Year Sky Maps

We present a determination of the cosmic microwave background dipole amplitude and direction from the COBE Differential Microwave Radiometers (DMR) first year of data. Data from the six DMR channels are consistent with a Doppler-shifted Planck function of dipole amplitude Delta T = 3.365 +/-0.027 mK toward direction (l,b) = (264.4 +/- 0.3 deg, 48.4 +/- 0.5 deg). The implied velocity of the Local Group with respect to the CMB rest frame is 627 +/- 22 km/s toward (l,b) = (276 +/- 3 deg, 30 +/- 3 deg). DMR has also mapped the dipole anisotropy resulting from the Earth's orbital motion about the Solar system barycenter, yielding a measurement of the monopole CMB temperature at 31.5, 53, and 90 GHz, to be 2.75 +/- 0.05 K.Comment: Post Script (4 figures) Ap J 419, 1-6 (1993