25 research outputs found
Can we create âElite Riceââa multifunctional crop for food, feed, and bioenergy production?
Get PDFCellulolytic and Xylanolytic Actinomycetes selection to degrade Lignocellulosic biomass of Robusta coffee pulp ( Coffea canephora
No full textEfficient biological pretreatment and bioconversion of corn cob by the sequential application of a Bacillus firmus K-1 cellulase-free xylanolytic enzyme and commercial cellulases
No full textOptimization of pretreatment process of cassava rhizome for bio-succinic fermentation by Actinobacillus succinogenes
No full textAlkali delignification and Bacillus sp. BMP01 hydrolysis of rice straw for enhancing biofuel yields
No full textCombinatorial application of ammonium carbonate and sulphuric acid pretreatment to achieve enhanced sugar yield from pine needle biomass for potential biofuelâethanol production
No full textThe Effect of the Addition of Acetic Acid to Aqueous Ionic Liquid Mixture Using Microwave-assisted Pretreatment in the Saccharification of Napier Grass
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Glyceraldehyde-3-phosphate dehydrogenase promoter from enoki mushroom drove gene expression of exogenous cellulase in Aspergillus niger
No full textOptimization of Cellulase Production by Halobacillus sp. QLS 31 Isolated from Lake Qarun, Egypt.
No full textA halophilic cellulase-producing bacterium was isolated from a sediment sample collected from Lake Qarun (Fayoum Province, Egypt). Molecular identification based on 16S rDNA amplification and sequencing revealed 99% homology with Halobacillus sp. and hence was designated as Halobacillus sp. QLS 31. Medium composition and culture conditions were optimized for enhancing the production of cellulase enzyme using the Plackett-Burman statistical design. Ten variables were evaluated for their influence on cellulase production. Carboxymethyl cellulose (CMC), zinc sulfate (ZnSO4), and inoculum size were found to exert a significant effect on cellulase productivity by Halobacillus sp. QLS 31. The maximum specific activity of cellulase enzyme was 48.08 U/mg. Following the predicted conditions, a 7.5-fold increase in cellulase specific activity (175.47 U/mg) was achieved compared to the basal medium (23.19 U/mg) under the following optimized conditions: temperature (30 °C), fermentation time (2 days ), pH value (9), CMC concentration (1%), inoculum size (1%), yeast extract concentration (0.1%), ammonium sulfate ((NH3)2SO4) concentration (0.1%), sodium chloride (NaCl) concentration (20%), and metal inducers: ZnSO4 (0.1%) and Ca/Mg ratio (0.01%). Thus, the results of this study provide an important basis for more efficient, cheap industrial cellulase production from halophilic Halobacillus sp. QLS 31
