Whole-genome sequencing and genome-scale metabolic modeling of Chromohalobacter canadensis 85B to explore its salt tolerance and biotechnological use

Salt tolerant organisms are increasingly being used for the industrial production of high‐value biomolecules due to their better adaptability compared to mesophiles. Chromohalobacter canadensis is one of the early halophiles to show promising biotechnology potential, which has not been explored to date. Advanced high throughput technologies such as whole‐genome sequencing allow in‐depth insight into the potential of organisms while at the frontiers of systems biology. At the same time, genome‐scale metabolic models (GEMs) enable phenotype predictions through a mechanistic representation of metabolism. Here, we sequence and analyze the genome of C. canadensis 85B, and we use it to reconstruct a GEM. We then analyze the GEM using flux balance analysis and validate it against literature data on C. canadensis. We show that C. canadensis 85B is a metabolically versatile organism with many features for stress and osmotic adaptation. Pathways to produce ectoine and polyhydroxybutyrates were also predicted. The GEM reveals the ability to grow on several carbon sources in a minimal medium and reproduce osmoadaptation phenotypes. Overall, this study reveals insights from the genome of C. canadensis 85B, providing genomic data and a draft GEM that will serve as the first steps towards a better understanding of its metabolism, for novel applications in industrial biotechnology

Biosurfactants’ Potential Role in Combating COVID-19 and Similar Future Microbial Threats

During 2020, the world has experienced extreme vulnerability in the face of a disease outbreak. The coronavirus disease 2019 (COVID-19) pandemic discovered in China and rapidly spread across the globe, infecting millions, causing hundreds of thousands of deaths, and severe downturns in the economies of countries worldwide. Biosurfactants can play a significant role in the prevention, control and treatment of diseases caused by these pathogenic agents through various therapeutic, pharmaceutical, environmental and hygiene approaches. Biosurfactants have the potential to inhibit microbial species with virulent intrinsic characteristics capable of developing diseases with high morbidity and mortality, as well as interrupting their spread through environmental and hygiene interventions. This is possible due to their antimicrobial activity, ability to interact with cells forming micelles and to interact with the immune system, and compatibility with relevant processes such as nanoparticle synthesis. They, therefore, can be applied in developing innovative and more effective pharmaceutical, therapeutics, sustainable and friendly environmental management approaches, less toxic formulations, and more efficient cleaning agents. These approaches can be easily integrated into relevant product development pipelines and implemented as measures for combating and managing pandemics. This review examines the potential approaches of biosurfactants as useful molecules in fighting microbial pathogens both known and previously unknown, such as COVID-19

Talaromyces aculeatus from acidic environment as a new fungal biosorbent for removal of some reactive textile dyes

Biosorption potential of Talaromayces aculeatus AMDC-14 (KF588645) as low-cost biosorbent for different dyes (Reactive Blue 13, Reactive Blue 72, Reactive Yellow 85 and Reactive Orange 13) removal from aqueous medium was studied. Experiments were performed to determine effect of initial solution pH (1-7), biosorbent dose (0.1-2.5 g L-1), time (15-1440 min), dye concentration (10-200 ppm) and temperature (25-40 °C) onto Talaromayces aculeatus biosorption.  Biosorption equilibrium data were described very well for Reactive Blue 13, Reactive Blue 72, Reactive Yellow 85 and Reactive Orange 13 by Langmuir isotherm model than Freundlich isotherm model with maximum biosorption capacities which are 32.10, 71.30, 20.67 and 31.30 mg g-1, respectively. Biosorption of investigated dyes fitted to kinetic model of pseudo-second-order for all dyes. Gibbs free energies (ΔG°) of biosorption processes were calculated and the results indicated that all selected dyes biosorption onto Talaromayces aculeatus AMDC-14 were spontaneous in this study. And also biosorption processes had endothermic enthalpy values except Reactive Blue 72

Simulated acid mine drainage treatment in iron oxidizing ceramic membrane bioreactor with subsequent co-precipitation of iron and arsenic

Acid mine drainage (AMD), generated in the active and abandoned mine sites, is characterized by low pH and high metal concentrations. One AMD treatment possibility is biologically oxidizing Fe2+ followed by precipitation through pH control. As compared to autotrophic iron oxidizing microbial community, a microbial community enriched in the presence of organic nutrients was hypothesized to yield higher biomass during commissioning the bioreactor. In this study, the treatment of Fe, Cu, Co, Mn, Zn, Ni, and As containing simulated AMD was studied using an iron-oxidizing ceramic membrane bioreactor (CMBR) at varying hydraulic retention times (HRTs) (6–24 h) and two different feed Fe2+ concentrations (250 and 750 mg/L). The impact of tryptone soya broth (TSB) on the CMBR performance was also investigated. Almost complete Fe2+ oxidation and sustainable flux at around 5.0 L/(m2.h) were obtained in the CMBR with the Alicyclobacillus tolerans and Acidiphilium cryptum dominated enrichment culture. The Fe2+ oxidation rate, as assessed in batch operation cycles of CMBR, increased significantly with increasing Fe2+ loading to the bioreactor. The iron oxidation rate decreased by the elimination of organic matter from the feed. The increase of the CMBR permeate pH to 3.5–4.0 resulted in selective co-precipitation of As and Fe (over 99%) with the generation of biogenic schwertmannite.acceptedVersionPeer reviewe

Bacterial Membrane Vesicles as Smart Drug Delivery and Carrier Systems: A New Nanosystems Tool for Current Anticancer and Antimicrobial Therapy

Bacterial membrane vesicles (BMVs) are known to be critical communication tools in several pathophysiological processes between bacteria and host cells. Given this situation, BMVs for transporting and delivering exogenous therapeutic cargoes have been inspiring as promising platforms for developing smart drug delivery systems (SDDSs). In the first section of this review paper, starting with an introduction to pharmaceutical technology and nanotechnology, we delve into the design and classification of SDDSs. We discuss the characteristics of BMVs including their size, shape, charge, effective production and purification techniques, and the different methods used for cargo loading and drug encapsulation. We also shed light on the drug release mechanism, the design of BMVs as smart carriers, and recent remarkable findings on the potential of BMVs for anticancer and antimicrobial therapy. Furthermore, this review covers the safety of BMVs and the challenges that need to be overcome for clinical use. Finally, we discuss the recent advancements and prospects for BMVs as SDDSs and highlight their potential in revolutionizing the fields of nanomedicine and drug delivery. In conclusion, this review paper aims to provide a comprehensive overview of the state-of-the-art field of BMVs as SDDSs, encompassing their design, composition, fabrication, purification, and characterization, as well as the various strategies used for targeted delivery. Considering this information, the aim of this review is to provide researchers in the field with a comprehensive understanding of the current state of BMVs as SDDSs, enabling them to identify critical gaps and formulate new hypotheses to accelerate the progress of the field