La céramique attique de Béziers (VIe-IVe s.).: Approche de la diffusion et de l'utilisation de la vaisselle attique en Languedoc occidental.

version intégrale avec deux figures supplémentaires (fig. 8 et 9)This study of a large part of the attic pottery imported at Béziers from the VIth century B. C., analyze the diffusion of this vases in the geographical and chronological context of the western Mediterranean (South of France and Spain). Typology and decoration (but without the attributions to the attic painters) are presented and compared to this imported ware for each site. The composition of the tableware in Languedoc-Roussillon is analyzed, as well as the little importance of the trade of Emporion in this land, traditionally considered as very strong. The indirect role of the Etruscans in the provisioning is proposed, even for the Vth century, when this vases were very abondant in Béziers and rather rares elsewhere in this area.Placée dans le contexte géographique et chronologique de la diffusion de la céramique attique en Méditerranée occidentale, l'étude concerne une part importante de celle qui est parvenue à Béziers à partir du VIe s. av. J.C. Présentés par formes, types et décors (mais en faisant l'impasse sur les attributions aux peintres), les vases attiques mis au jour sur ce site sont comparés à ceux importés ailleurs dans le Midi de la France et en Espagne. Une analyse de leur place dans le cadre du service de table est proposée. Le rôle d'Emporion dans l'approvisionnement de Béziers et du Languedoc occidental (longtemps considéré comme prépondérant) semble en réalité plutôt réduit alors que celui des Étrusques – indirect – est envisagé, même pour le plein Ve s. av. J.-C., qui, de manière originale par rapport au reste de l'aire géographique, correspond à la période de plus grande abondance à Béziers

Autour de la fondation de Narbo Martius : Atacini et autres peuples préromains de l'Aude.

International audienceLes Atacini (“Audois”) sont mentionnés une seule fois dans la littérature antique. Associés aux vétérans de la Xe légion (“Decimani”) lors de la re-fondation coloniale de Narbonne, ces indigènes du bassin de l'Aude ont dû faire partie des peuples “obscurs” que Strabon situait entre le Rhône et les Pyrénées et qu'il n'a pas assimilés aux Volques. Très tôt intégrés dans l'organisation coloniale, le fait de les désigner par un nom à valeur simplement géographique traduit sans doute l'importance politique limitée dont ils ont bénéficié entre la fin du IIe s. av. J.-C. et le milieu du siècle suivant

High-sensitivity staining of proteins for one- and two-dimensional gel electrophoresis using post migration covalent staining with a ruthenium fluorophore.

This paper describes the use of a ruthenium complex ((bis(2,2'-bipyridine)-4'-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate), abbreviated below as ASCQ_Ru) commercially available and chemically pure. This new ruthenium complex ASCQ_Ru brings an activated ester, allowing the selective acylation of amino acid side chain amines for the post migration staining of proteins separated in 1-DE and 2-DE. The protocol used is a simple three-step protocol fixing the proteins in the gel, staining and then washing, as no lengthy destaining step is required. First the critical staining step was optimized. Although in solution the best described pH for acylating proteins with this reagent is phosphate buffer at pH 7.0, we found that best medium for in-gel staining is unbuffered ACN/water solution (20/80 v/v). The two other steps are less critical and classical conditions are satisfactory: fixing with 7% acetic acid/10% ethanol solution and washing four times for 10 min with water. Sensitivity tests were performed using 1-DE on protein molecular weight markers. We obtained a higher sensitivity than SYPRO Ruby with a detection limit of 80 pg of protein per well. However, contrary to SYPRO Ruby, ASCQ_Ru exhibits a logarithmic dependency on the amount of protein. The dynamic range is similar to SYPRO Ruby and is estimated between three and four orders of magnitude. Finally, the efficiency of the post migration ASCQ_Ru staining for 2-D gel separation is demonstrated on the whole protein extract from human colon carcinoma cells lines HCT 116. ASCQ_Ru gave the highest number of spot detected compared to other common stains Colloidal CBB, SYPRO Ruby and Deep Purple

Bridging intravenous thrombolysis in patients with atrial fibrillation

Atrial fibrillation; Intravenous thrombolysis; Oral anticoagulationFibrilación auricular; Trombólisis intravenosa; Anticoagulación oralFibril·lació auricular; Trombolisi intravenosa; Anticoagulació oralBackground and purpose: 40% of acute ischemic stroke patients treated by mechanical thrombectomy (MT) have a clinical history of atrial fibrillation (AF). The safety of bridging intravenous thrombolysis (IVT) (MT + IVT) is currently being discussed. We aimed to analyze the interaction between oral anticoagulation (OAC) status or AF with bridging IVT, regarding the occurrence of symptomatic intracranial hemorrhage (sICH) and functional outcome. Materials and Methods: Multicentric observational cohort study (BEYOND-SWIFT registry) of consecutive patients undergoing MT between 2010 and 2018 (n = 2,941). Multinomial regression models were adjusted for prespecified baseline and plausible pathophysiological covariates identified on a univariate analysis to assess the association of AF and OAC status with sICH and good outcomes (90-day modified Rankin Scale score 0–2). Results: In the total cohort (median age 74, 50.6% women), 1,347 (45.8%) patients had AF. Higher admission National Institutes of Health Stroke Scale (NIHSS) score (aOR 1.04 [95% 1.02–1.06], per point of increase) and prior medication with Vitamin K antagonists (VKA) (aOR 2.19 [95% 1.27–3.66]) were associated with sICH. Neither AF itself (aOR 0.71 [95% 0.41–1.24]) nor bridging IVT (aOR 1.08 [0.67–1.75]) were significantly associated with increased sICH. Receiving bridging IVT (aOR 1.61 [95% 1.24–2.11]) was associated with good 90-day outcome, with no interaction between AF and IVT (p = 0.92). Conclusion: Bridging IVT appears to be a reasonable clinical option in selected patients with AF. Given the increased sICH risk in patients with VKA, subgroup analysis of the randomized controlled trials should analyze whether patients with VKA might benefit from withholding bridging IVT.This study was funded by the Bangerter-Rhyner Foundation and the Swiss Academy of Medical Sciences. Open access funding provided by University of Bern

Affine Toda field theories related to Coxeter groups of non-crystallographic type

We propose affine Toda field theories related to the non-crystallographic Coxeter groups H_2, H_3 and H_4. The classical mass spectrum, the classical three-point couplings and the one-loop corrections to the mass renormalisation are determined. The construction is carried out by means of a reduction procedure from crystallographic to non-crystallographic Coxeter groups. The embedding structure explains for various affine Toda field theories that their particles can be organised in pairs, such that their relative masses differ by the golden ratio.Comment: 28 pages LaTe

Allegory and animals in Olive Schreiner’s Undine : A Queer Little Child (1929)

Written and abandoned in the 1870s, and published posthumously in 1929, Undine: A Queer Little Child has remained on the margins of Olive Schreiner (1855-1920) studies, repeatedly dismissed as a juvenile and poor antecedent to The Story of An African Farm (1883), or deemed valuable primarily for its autobiographical content. This article redresses these schematic readings by analysing how Schreiner draws on allegorical forms in order to explore aspects of her burgeoning radicalism. Focusing on one of the main allegorical thrusts of the novel, provided by the zoomorphic and anthropomorphic animal characters that descend from mythical, fairytale, and Ancient Greek philosophical origins, it investigates how the protagonist’s metaphorically significant associations with animals relate to freethinking, feminist, and anti-imperialist ideas introduced by the novel. Undine thus undermines dominant nineteenth-century models of the “primitive” human or animal as less evolutionarily developed and without political platform, which can be seen to be a liberating move when the novel is read in dialogue with Jacques Derrida’s lectures on animals, and with other recent work in postcolonial ecocriticism

The Deuteron Confronts Big Bang Nucleosynthesis

Recent determinations of the deuterium abundance, $^2$H/H, in high redshift Lyman limit hydrogen clouds challenge the usual picture of primordial nucleosynthesis based on \lq\lq concordance\rq\rq\ of the calculated light element ($^2$H, $^3$He, $^4$He, $^7$Li) nucleosynthesis yields with the observationally-inferred abundances of these species. Concordance implies that all light element yields can be made to agree with the observationally-inferred abundances (within errors) for single global specifications of the baryon-to-photon ratio, $\eta$; lepton number; neutron lifetime; and expansion rate (or equivalently, effective number of light neutrino degrees of freedom $N_{\nu}$). Though one group studying Lyman limit systems obtains a high value of $^2$H/H ($\sim 2\times {10}^{-4}$), another group finds consistently low values ($\sim 2\times {10}^{-5}$). In the former case, concordance for $N_{\nu} =3$ is readily attained for the current observationally-inferred abundances of $^4$He and $^7$Li. But if the latter case represents the primordial deuterium abundance, then concordance for {\it any} $N_{\nu}$ is impossible unless the primordial value of $^7$Li/H is considerably larger than the abundance of lithium as measured in old, hot Pop II halo stars. Furthermore, concordance with $N_{\nu}=3$ is possible for low $^2$H/H only if either (1) the primordial $^4$He abundance has been significantly underestimated, or (2) new neutrino sector physics is invoked. We argue that systematic underestimation of both the $^7$Li and $^4$He primordial abundances is the likely resolution of this problem, a conclusion which is strengthened by new results on $^4$He.Comment: To be published in Nucl. Phys. B (Proc. Suppl.), in the proceedings of "Sources and Detection of Dark Matter in the Universe", held in Santa Monica, Feb. 14-16 1996. 5 pages. Replaced version has a TeX command removed that apparently caused some latex compilers to fai

Suzaku and BeppoSAX X-ray Spectra of the Persistently Accreting Neutron-Star Binary 4U 1705-44

We present an analysis of the broad-band spectra of 4U~1705--44 obtained with {\it Suzaku} in 2006--2008 and by {\it BeppoSAX} in 2000. The source exhibits two distinct states: the hard state shows emission from 1 to 150 keV, while the soft state is mostly confined to be $<40$ keV. We model soft-state continuum spectra with two thermal components, one of which is a multicolor accretion disk and the other is a single-temperature blackbody to describe the boundary layer, with additional weak Comptonization represented by either a simple power law or the SIMPL model by Steiner et al. The hard-state continuum spectra are modeled by a single-temperature blackbody for the boundary layer plus strong Comptonization, modeled by a cutoff power law. While we are unable to draw firm conclusions about the physical properties of the disk in the hard state, the accretion disk in the soft state appears to approximately follow $L\propto T^{3.2}$. The deviation from $L\propto T^4$, as expected from a constant inner disk radius, might be caused by a luminosity-dependent spectral hardening factor and/or real changes of the inner disk radius in some part of the soft state. The boundary layer apparent emission area is roughly constant from the hard to the soft states, with a value of about 1/11 of the neutron star surface. The magnetic field on the surface of the NS in 4U~1705--44 is estimated to be less than about $1.9\times 10^8$ G, assuming that the disk is truncated by the ISCO or by the neutron star surface. Broad relativistic Fe lines are detected in most spectra and are modeled with the diskline model. The strength of the Fe lines is found to correlate well with the boundary layer emission in the soft state. In the hard state, the Fe lines are probably due to illumination of the accretion disk by the strong Comptonization emission.Comment: Accepted for publication in the Astrophysical Journa

KRASG12C/TP53 co-mutations identify long-term responders to first line palliative treatment with pembrolizumab monotherapy in PD-L1 high (≥50%) lung adenocarcinoma

Background: Pembrolizumab is a standard of care as first line palliative therapy in PD-L1 overexpressing (≥50%) non-small cell lung cancer (NSCLC). This study aimed at the identification of KRAS and TP53-defined mutational subgroups in the PD-L1 high population to distinguish long-term responders from those with limited benefit. Methods: In this retrospective, observational study, patients from 4 certified lung cancer centers in Berlin, Germany, having received pembrolizumab monotherapy as first line palliative treatment for lung adenocarcinoma (LuAD) from 2017 to 2018, with PD-L1 expression status and targeted NGS data available, were evaluated. Results: A total of 119 patients were included. Rates for KRAS, TP53 and combined mutations were 52.1%, 47.1% and 21.9%, respectively, with no association given between KRAS and TP53 mutations (P=0.24). By trend, PD-L1 expression was higher in KRAS-positive patients (75% vs. 65%, P=0.13). Objective response rate (ORR), median progression-free survival (PFS) and overall survival (OS) in the KRASG12C group (n=32, 51.6%) were 63.3%, 19.8 months (mo.) and not estimable (NE), respectively. Results in KRASother and wild type patients were similar and by far lower (42.7%, P=0.06; 6.2 mo., P<0.001; 23.4 mo., P=0.08). TP53 mutations alone had no impact on response and survival. However, KRASG12C/TP53 co-mutations (n=12) defined a subset of long-term responders (ORR 100.0%, PFS 33.3 mo., OS NE). In contrast, patients with KRASother/TP53 mutations showed a dismal prognosis (ORR 27.3%, P=0.002; PFS 3.9 mo., P=0.001, OS 9.7 mo., P=0.02). Conclusions: A comprehensive assessment of KRAS subtypes and TP53 mutations allows a highly relevant prognostic differentiation of patients with metastatic, PD-L1 high LuAD treated upfront with pembrolizumab

New Microbicidal Functions of Tracheal Glands: Defective Anti-Infectious Response to Pseudomonas aeruginosa in Cystic Fibrosis

Tracheal glands (TG) may play a specific role in the pathogenesis of cystic fibrosis (CF), a disease due to mutations in the cftr gene and characterized by airway inflammation and Pseudomonas aeruginosa infection. We compared the gene expression of wild-type TG cells and TG cells with the cftr ΔF508 mutation (CF-TG cells) using microarrays covering the whole human genome. In the absence of infection, CF-TG cells constitutively exhibited an inflammatory signature, including genes that encode molecules such as IL-1α, IL-β, IL-32, TNFSF14, LIF, CXCL1 and PLAU. In response to P. aeruginosa, genes associated with IFN-γ response to infection (CXCL10, IL-24, IFNγR2) and other mediators of anti-infectious responses (CSF2, MMP1, MMP3, TLR2, S100 calcium-binding proteins A) were markedly up-regulated in wild-type TG cells. This microbicidal signature was silent in CF-TG cells. The deficiency of genes associated with IFN-γ response was accompanied by the defective membrane expression of IFNγR2 and altered response of CF-TG cells to exogenous IFN-γ. In addition, CF-TG cells were unable to secrete CXCL10, IL-24 and S100A8/S100A9 in response to P. aeruginosa. The differences between wild-type TG and CF-TG cells were due to the cftr mutation since gene expression was similar in wild-type TG cells and CF-TG cells transfected with a plasmid containing a functional cftr gene. Finally, we reported an altered sphingolipid metabolism in CF-TG cells, which may account for their inflammatory signature. This first comprehensive analysis of gene expression in TG cells proposes a protective role of wild-type TG against airborne pathogens and reveals an original program in which anti-infectious response was deficient in TG cells with a cftr mutation. This defective response may explain why host response does not contribute to protection against P. aeruginosa in CF