Development of Antibody-Coated Magnetite Nanoparticles for Biomarker Immobilization

Magnetic nanoparticles (MNPs) have great potential in biomedical applications because of their magnetic response offers the possibility to direct them to specific areas and target biological entities. Magnetic separation of biomolecules is one of the most important applications of MNPs because their versatility in detecting cancer biomarkers. However, the effectiveness of this method depends on many factors, including the type of functionalization onto MNPs. Therefore, in this study, magnetite nanoparticles have been developed in order to separate the 5′-nucleotidase enzyme (5eNT). The 5eNT is used as a bio-indicator for diagnosing diseases such as hepatic ischaemia, liver tumor, and hepatotoxic drugs damage. Magnetic nanoparticles were covered in a core/shell type with silica, aminosilane, and a double shell of silica-aminosilane. A ScFv (fragment antibody) and anti-CD73 antibody were attached to the coated nanoparticles in order to separate the enzyme. The magnetic separation of this enzyme with fragment antibody was found to be 28% higher than anti-CD73 antibody and the enzyme adsorption was improved with the double shell due to the increased length of the polymeric chain. Magnetite nanoparticles with a double shell (silica-aminosilane) were also found to be more sensitive than magnetite with a single shell in the detection of biomarkers

Avaliação físico-química de compósitos madeira-plástico aplicados ao design de produtos

Wood waste is commonly treated as waste, and there is a problem for its disposal. Currently, fibers are used to produce paper, building material, and bioplastics, although wood-plastic composite materials have previously been developed, there are no reports of one with a polycaprolactone matrix. During this research, polycaprolactone-poplar wood composite materials were developed that were characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, biodegradation, water uptake test, three-point bending mechanical properties, and temperature sweeping. Scanning electron microscopy images showed composite materials in a uniform matrix, spectroscopy showed the interaction of the carbonyl group of polycaprolactone and the stretch bond O-C-O with the OH groups of wood. Composite materials are resistant to hydrolytic degradation even under the effects of UV rays. According to the results, an appropriate material was obtained for product design, so a design proposal for it was eventually generated.A eliminação de resíduos de madeira, comumente tratados como desperdício, ocasiona problemas em seu processo de descarte. Atualmente, as fibras são utilizadas na produção de papel, material de construção e bioplásticos; embora materiais compostos de madeira-plástico tenham sido desenvolvidos anteriormente, não há relatos de algum com matriz de policaprolactona. Durante a presente pesquisa,foram desenvolvidos materiais compósitos de policaprolactona-farinha de madeira de choupo que foramcaracterizados por meio de microscopia eletrônica de varredura, espectroscopia de infravermelho por transformada de Fourier, biodegradação, teste de absorção de água, propriedades mecânicas de flexão em três pontos e varredura de temperatura. Imagens de microscopia eletrônica mostraram materiais compostos com uniformidade na matriz, a espectroscopia mostrou interação do grupo carbonil de policaprolactona e da ligação elástica O-C-O com os grupos de madeira OH. Os materiais compostos são resistentes à degradação hidrolítica mesmo sob os efeitos dos raios UV. De acordo com os resultados alcançados, foi obtido um material adequado para uso em projeto de produto, para o qual foi finalmente gerada uma proposta formal de design.  Los residuos de madera son tratados comúnmente como desecho y existe un problema para su disposición. Actualmente, las fibras son utilizadas para producir papel, material de construcción y bioplásticos; aunque han sido desarrollados previamente materiales compuestos madera-plástico, no hay reportes de uno con matriz de policaprolactona. Durante la investigación se desarrollaron materiales compuestos policaprolactona-harina de madera de álamo que fueron caracterizados por medio de microscopía electrónica de barrido, espectroscopía infrarrojo por transformada de Fourier, biodegradación, prueba de captación de agua, propiedades mecánicas de flexión a tres puntos y barrido de temperatura. Las imágenes de microscopía electrónica de barrido mostraron materiales compuestos con uniformidad en la matriz, la espectroscopía evidenció interacción del grupo carbonilo de la policaprolactona y el enlace de estiramiento O-C-O con los grupos OH de la madera. Los materiales compuestos son resistentes a la degradación hidrolítica aún bajo los efectos de los rayos UV. De acuerdo con los resultados, se obtuvo un material apropiado para su uso en diseño de productos, por lo que finalmente fue generada una propuesta de diseño del mismo

Chitosan/Poly(Dllactide-Co-Glycolide) Scaffolds for Tis- sue Engineering

Abstract Chitosan/poly(DL-lactide-co-glycolide) (Ch/DL PLG) composite scaffolds were fabricated by freeze-drying lyophilization, and were evaluated and compared for use as a bone regeneration scaffold through measurements of the compression mechanical properties of the porous scaffolds. Also, In vitro cell culture of Sprague-Dawley rat's osteoblasts were used to evaluate the phenotype expression of cells in the scaffolds, characterizing the cellular adhesion, proliferation and alkaline phosphatase activity. The gene expression of osteocalcin, sialoprotein, alkaline phosphatase, Type I collagen and TGFb1 were confirmed in the samples; moreover, it was confirmed, the mineralization by IR spectra and EDS analysis. Our results thus show that Ch/DL PLG scaffolds are suitable for biological applications