ETHNOBOTANICAL STUDY IN TOLEDO-PR DISTRICT: AN APPROACH ABOUT USED SPECIES VARIABILITY WITH THE SAME POPULAR NAME

The ethnopharmacological knowledge acquired throughout human evolution culminated in the development of high therapeutic relevance drugs. In ethnobotanical survey conducted in the city of Toledo in 2007, it was found that 79% of the population often use plants for medicinal purposes, mostly in the tea form, originated from own crops. They claimed that the consumption of these plants improved their health conditions. The plants mainly cited were: balm, chamomile and marcela. This study aimed to verify if the population habits related to medicinal plants have changed, also  proceed a literature review to find out if the most used plants have their biological activity scientifically proven and looking for  reports of homonymous to the most cited medicinal plants. There was small change in the species used by the population. In the literature, it was found out that different species are used with the same popular name, in practice, it was detected the use of two different species, Piper amalago L. (UNOP 7972) and Piper mikanianum (Kunth) (UNOP 7971), for  the medicinal plant commonly known as pariparoba, although none of them is recognized for the Brazilian Pharmacopoeia

Observations of the planetary nebula RWT 152 with OSIRIS/GTC

10 pages, 6 figuresRWT 152 is one of the few known planetary nebulae with an sdO central star. We present subarcsecond red tunable filterHa imaging and intermediate-resolution, long-slit spectroscopy of RWT 152 obtained with OSIRIS/GTC (Optical System for Imaging and low-Intermediate-Resolution Integrated Spectroscopy/Gran Telescopio Canarias) with the goal of analysing its properties. The Ha image reveals a bipolar nebula with a bright equatorial region and multiple bubbles in the main lobes. A faint circular halo surrounds the main nebula. The nebular spectra reveal a very low excitation nebula with weak emission lines from H+, He+ and double-ionized metals, and absence of emission lines from neutral and single-ionized metals, except for an extremely faint [NII] lambda 6584 emission line. These spectra may be explained if RWT 152 is a density-bounded planetary nebula. Low nebular chemical abundances of S, O, Ar, N and Ne are obtained in RWT 152, which, together with the derived high peculiar velocity (similar to 92-131 km s(-1)), indicate that this object is a halo planetary nebula. The available data are consistent with RWT 152 evolving from a low-mass progenitor (similar to 1 M-circle dot) formed in a metal-poor environment.This paper has been partially supported by grant AYA 2011-24052 (AA, ES), AYA 2011-30228-C3-01 (LFM), ESP2014-55996-C2-2-R (AU) and AYA2014-57369-C3-3-P (LFM) of the Spanish MINECO (all them co-funded by FEDER funds). We also acknowledge support from grant IN-CITE09 312191PR (AU, LFM, AA) of Xunta de Galicia, partially funded by FEDER funds, from grant PRX15/00511 (AU) of the Spanish MECD, and from grant 12VI20 (AU, LFM, AA) of the University of Vigo. AA also acknowledges support from FONDECYT through postdoctoral grant 3160364. This research has made use of the SIMBAD data base, operated at the CDS, Strasbourg (France), Aladin, NASA's Astrophysics Data System Bibliographic Services and the Spanish Virtual Observatory supported from the Spanish MINECO through grant AYA2011-24052.Peer reviewe

Local structure adaptability through multi cations for oxygen redox accommodation in Li-Rich layered oxides

Stable lattice oxygen redox (l-OR) is the key enabler for achieving attainable high energy density in Li-rich layered oxide cathode materials for Li-ion batteries. However, the unique local structure response to oxygen redox in these materials, resulting in energy inefficiency and hysteresis, still remains elusive, preventing their potential applications. By combining the state-of-the-art neutron pair distribution function with crystal orbital overlap analysis, we directly observe the distinct local structure adaption originated from the potential O–O chemical bonds. The structure adaptability is optimized based on the nature of multi transition metals in our model compound Li1.2Ni0.13Mn0.54Co0.13O2, which accommodates the oxygen redox and at the same time preserves the global layered structure. These findings not only advance the understanding of l-OR, but also provide new perspectives in the rational design of high-energy-density cathode materials with reversible and stable l-OR

Integrated Functions of Pax3 and Pax7 in the Regulation of Proliferation, Cell Size and Myogenic Differentiation

Pax3 and Pax7 are paired-box transcription factors with roles in developmental and adult regenerative myogenesis. Pax3 and Pax7 are expressed by postnatal satellite cells or their progeny but are down regulated during myogenic differentiation. We now show that constitutive expression of Pax3 or Pax7 in either satellite cells or C2C12 myoblasts results in an increased proliferative rate and decreased cell size. Conversely, expression of dominant-negative constructs leads to slowing of cell division, a dramatic increase in cell size and altered morphology. Similarly to the effects of Pax7, retroviral expression of Pax3 increases levels of Myf5 mRNA and MyoD protein, but does not result in sustained inhibition of myogenic differentiation. However, expression of Pax3 or Pax7 dominant-negative constructs inhibits expression of Myf5, MyoD and myogenin, and prevents differentiation from proceeding. In fibroblasts, expression of Pax3 or Pax7, or dominant-negative inhibition of these factors, reproduce the effects on cell size, morphology and proliferation seen in myoblasts. Our results show that in muscle progenitor cells, Pax3 and Pax7 function to maintain expression of myogenic regulatory factors, and promote population expansion, but are also required for myogenic differentiation to proceed

ALMA-IMF IX: Catalog and Physical Properties of 315 SiO Outflow Candidates in 15 Massive Protoclusters

We present a catalog of 315 protostellar outflow candidates detected in SiO J=5-4 in the ALMA-IMF Large Program, observed with ~2000 au spatial resolution, 0.339 km/s velocity resolution, and 2-12 mJy/beam (0.18-0.8 K) sensitivity. We find median outflow masses, momenta, and kinetic energies of ~0.3 M$_{\odot}$, 4 M$_{\odot}$ km/s, and 10$^{45}$ erg, respectively. Median outflow lifetimes are 6,000 years, yielding median mass, momentum, and energy rates of $\dot{M}$ = 10$^{-4.4}$ M$_{\odot}$ yr$^{-1}$, $\dot{P}$ = 10$^{-3.2}$ M$_{\odot}$ km/s yr$^{-1}$, and $\dot{E}$ = 1 L$_{\odot}$. We analyze these outflow properties in the aggregate in each field. We find correlations between field-aggregated SiO outflow properties and total mass in cores (~3$-$5$\sigma$), and no correlations above 3$\sigma$ with clump mass, clump luminosity, or clump luminosity-to-mass ratio. We perform a linear regression analysis and find that the correlation between field-aggregated outflow mass and total clump mass - which has been previously described in the literature - may actually be mediated by the relationship between outflow mass and total mass in cores. We also find that the most massive SiO outflow in each field is typically responsible for only 15-30% of the total outflow mass (60% upper limit). Our data agree well with the established mechanical force-bolometric luminosity relationship in the literature, and our data extend this relationship up to L $\geq$ 10$^6$ L$_{\odot}$ and $\dot{P}$ $\geq$ 1 M$_{\odot}$ km/s yr$^{-1}$. Our lack of correlation with clump L/M is inconsistent with models of protocluster formation in which all protostars start forming at the same time.Comment: 46 pages, 14 figures, 10 tables. This publication has an associated Zenodo entry, which can be found here: https://zenodo.org/records/835059

ALMA-IMF. VII. First release of the full spectral line cubes: Core kinematics traced by DCN J=(3-2)

ALMA-IMF is an Atacama Large Millimeter/submillimeter Array (ALMA) Large Program designed to measure the core mass function (CMF) of 15 protoclusters chosen to span their early evolutionary stages. It further aims to understand their kinematics, chemistry, and the impact of gas inflow, accretion, and dynamics on the CMF. We present here the first release of the ALMA-IMF line data cubes (DR1), produced from the combination of two ALMA 12m-array configurations. The data include 12 spectral windows, with eight at 1.3mm and four at 3mm. The broad spectral coverage of ALMA-IMF (~6.7 GHz bandwidth coverage per field) hosts a wealth of simple atomic, molecular, ionised, and complex organic molecular lines. We describe the line cube calibration done by ALMA and the subsequent calibration and imaging we performed. We discuss our choice of calibration parameters and optimisation of the cleaning parameters, and we demonstrate the utility and necessity of additional processing compared to the ALMA archive pipeline. As a demonstration of the scientific potential of these data, we present a first analysis of the DCN (3-2) line. We find that DCN traces a diversity of morphologies and complex velocity structures, which tend to be more filamentary and widespread in evolved regions and are more compact in the young and intermediate-stage protoclusters. Furthermore, we used the DCN (3-2) emission as a tracer of the gas associated with 595 continuum cores across the 15 protoclusters, providing the first estimates of the core systemic velocities and linewidths within the sample. We find that DCN (3-2) is detected towards a higher percentage of cores in evolved regions than the young and intermediate-stage protoclusters and is likely a more complete tracer of the core population in more evolved protoclusters. The full ALMA 12m-array cubes for the ALMA-IMF Large Program are provided with this DR1 release.Comment: 75 pages (21 main body; 54 appendix), 37 figures. The ALMA-IMF DR1 line release is hosted at https://dataverse.harvard.edu/dataverse/alma-im

Structural and Functional Evaluation of C. elegans Filamins FLN-1 and FLN-2

Filamins are long, flexible, multi-domain proteins composed of an N-terminal actin-binding domain (ABD) followed by multiple immunoglobulin-like repeats (IgFLN). They function to organize and maintain the actin cytoskeleton, to provide scaffolds for signaling components, and to act as mechanical force sensors. In this study, we used transcript sequencing and homology modeling to characterize the gene and protein structures of the C. elegans filamin orthologs fln-1 and fln-2. Our results reveal that C. elegans FLN-1 is well conserved at the sequence level to vertebrate filamins, particularly in the ABD and several key IgFLN repeats. Both FLN-1 and the more divergent FLN-2 colocalize with actin in vivo. FLN-2 is poorly conserved, with at least 23 IgFLN repeats interrupted by large regions that appear to be nematode-specific. Our results indicate that many of the key features of vertebrate filamins are preserved in C. elegans FLN-1 and FLN-2, and suggest the nematode may be a very useful model system for further study of filamin function

Dlk1 Is Necessary for Proper Skeletal Muscle Development and Regeneration

Delta-like 1homolog (Dlk1) is an imprinted gene encoding a transmembrane protein whose increased expression has been associated with muscle hypertrophy in animal models. However, the mechanisms by which Dlk1 regulates skeletal muscle plasticity remain unknown. Here we combine conditional gene knockout and over-expression analyses to investigate the role of Dlk1 in mouse muscle development, regeneration and myogenic stem cells (satellite cells). Genetic ablation of Dlk1 in the myogenic lineage resulted in reduced body weight and skeletal muscle mass due to reductions in myofiber numbers and myosin heavy chain IIB gene expression. In addition, muscle-specific Dlk1 ablation led to postnatal growth retardation and impaired muscle regeneration, associated with augmented myogenic inhibitory signaling mediated by NF-κB and inflammatory cytokines. To examine the role of Dlk1 in satellite cells, we analyzed the proliferation, self-renewal and differentiation of satellite cells cultured on their native host myofibers. We showed that ablation of Dlk1 inhibits the expression of the myogenic regulatory transcription factor MyoD, and facilitated the self-renewal of activated satellite cells. Conversely, Dlk1 over-expression inhibited the proliferation and enhanced differentiation of cultured myoblasts. As Dlk1 is expressed at low levels in satellite cells but its expression rapidly increases upon myogenic differentiation in vitro and in regenerating muscles in vivo, our results suggest a model in which Dlk1 expressed by nascent or regenerating myofibers non-cell autonomously promotes the differentiation of their neighbor satellite cells and therefore leads to muscle hypertrophy

The JCMT Plane Survey: First complete data release - emission maps and compact source catalogue

We present the first data release of the James Clerk Maxwell Telescope (JCMT) Plane Survey (JPS), the JPS Public Release 1 (JPSPR1). JPS is an 850-µm continuum survey of six fields in the northern inner Galactic Plane in a longitude range of ℓ = 7°–63°, made with the Sub-millimetre Common-User Bolometer Array 2 (SCUBA-2). This first data release consists of emission maps of the six JPS regions with an average pixel-to-pixel noise of 7.19 mJy beam−1, when smoothed over the beam, and a compact-source catalogue containing 7,813 sources. The 95 per cent completeness limits of the catalogue are estimated at 0.04 Jy beam−1 and 0.3 Jy for the peak and integrated flux densities, respectively. The emission contained in the compact-source catalogue is 42 ± 5 per cent of the total and, apart from the large-scale (greater than 8 arcmin) emission, there is excellent correspondence with features in the 500-µm Herschel maps. We find that, with two-dimensional matching, 98 ± 2 per cent of sources within the fields centred at ℓ = 20°, 30°, 40° and 50° are associated with molecular clouds, with 91 ± 3 per cent of the ℓ = 30° and 40° sources associated with dense molecular clumps. Matching the JPS catalogue to Herschel 70-µm sources, we find that 38 ± 1 per cent of sources show evidence of ongoing star formation. The images and catalogue will be a valuable resource for studies of star formation in the Galaxy and the role of environment and spiral arms in the star formation process